Influence of Polar Solutions on the Extraction of Phenolic Compounds from Capulín Fruits (Prunus serotina)

Hernández-Rodríguez, Guillermina; Espinosa-Solares, Teodoro; Hernández-Eugenio, Guadalupe; Villa-García, Matilde; Reyes-Trejo, Benito; Guerra-Ramı́rez, Diana

doi:10.29356/jmcs.v60i2.76

Cited by 7 publications

(9 citation statements)

References 12 publications

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“…Herein, we used a mixed-method approach [19] integrating both qualitative and quantitative methods for sample collection, chemical analysis, correlation analysis, and result interpretation. Optimum ultrasonic extraction conditions efficiently obtained via Taguchi experiment design [20] was adopted for antioxidant extraction first. The preliminary chemical assay results were further confirmed by high-performance liquid chromatograph−photodiode array detector (HPLC-PAD) instrumental analysis and correlation analysis to find out the major active compounds and components causative to the AA.…”

Section: Introductionmentioning

confidence: 99%

Correlation Study of Antioxidant Activity with Phenolic and Flavonoid Compounds in 12 Indonesian Indigenous Herbs

2021

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The antioxidant activity (AA), total phenolic content (TPC), and total flavonoid content (TFC) of selected Indonesian Zingiberaceae herbs were determined. An optimization extraction procedure was conducted by using Taguchi L16 orthogonal array. Four chemical assays were applied, including 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay, H2O2 scavenging activity assay, Folin–Ciocalteau (F–C) assay, and NaNO2-AlCl3-NaOH assay, which revealed remarkable differences in AA, TPC, and TFC. The result indicated the diversity of AA composition among the herbs, and C. longa exhibited the highest AA. HPLC-PAD analysis revealed that curcumin was present in five high antioxidant herbs, and the highest amount was in C. longa. Pearson correlation analysis indicated that the identified TPC and TFC were significant contributors to AA, and curcumin was likely the main contributing antioxidant compound. Our approach concluded that C. longa is the greatest source of natural antioxidants among 12 Indonesian indigenous Zingiberaceae herbs. The use of a mixed-method approach to augment the findings of solitary methods might facilitate future researchers to uncover deeper and hidden meanings.

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Section: Introductionmentioning

confidence: 99%

Correlation Study of Antioxidant Activity with Phenolic and Flavonoid Compounds in 12 Indonesian Indigenous Herbs

2021

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“…According to Hassimotto [16] the main phenolic compounds present in the pulp of A. muricate are the catechins, highly soluble in polar solvents such as methanol. Hernández-Rodríguez [23] evaluated the effect of different solvents and extraction time on the extraction yield of total phenolic compounds in fruits of Prunus serotina Erhr, reporting however that there is not significant statistical difference using acetone-water (7:3 v/v) and methanol-water (4:1 v/v) while the optimal extraction time was 30 min. Several authors have reported the use of selective extractions for flavonoids, Henry-García [10] optimized the extraction process of flavonoids present in leaves of A. muricana, reporting an extraction efficiency of 87% using 96% ethanol as solvent.…”

Section: Discussionmentioning

confidence: 99%

Total Phenols, Flavonoids and Antioxidant Activity in Annona muricata and Annona purpurea Callus Culture

Ov¹,

o-Dom韓guez²,

Luj醤-Hidalgo³

et al. 2019

Phyton

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Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog (MS) medium supplemented with different concentrations of 1-naphthylacetic acid (NAA), 6-benzyladenine (BA) and 2,4dichlorophenoxyacetic acid (2,4-D) utilized hypocotyls with explant. The highest percentage of callus formation was the treatment supplemented with 3 mg L-1 NAA for A. muricata (100%) while for A. purpurea in lower percentage (75%). BA stimulated the formation of shoots in all the evaluated concentrations, being the concentration of 2 mg L-1 the one that induced the greater formation of shoots for A. muricata (23 shoots/explant) and A. purpurea (28 shoots/explant). The content of total phenols, flavonoids and antioxidant activity was measured in the callus obtained from both species. The results showed that a higher content of total phenols was quantified in callus of A. purpurea (27.8 mg g-1 dw) compared to A. muricata (23.2 mg g-1 dw). The highest content of total flavonoids was observed in the callus of A. purpurea (8.0 μg g-1 dw). Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydracil radical assay. The concentration required for 50% inhibition (IC50) of the 2,2-diphenyl-1-picrylhydracil radicals were 4.22 μg mL-1 in methanolic extracts of callus of A. muricata, while in extracts of callus of A. purpurea was 2.86 μg mL-1 , in both cases was greater than that found for leaves. Callus culture of the species studied in this work represents an alternative for the production of natural antioxidants.

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“…Las muestras de fresa y cilantro previamente desinfectadas fueron extraídas de acuerdo con el método de (Hernández-Rodríguez et al, 2016). Primero se mezclaron con metanol al 80% en una relación 1/10 (p/v), ajustando el pH a 3 con HCl al 10%.…”

Section: Preparación De Extractosunclassified

Eficacia de métodos de desinfección y los efectos sobre las propiedades nutracéuticas en cilantro y fresa

Salgado-Escobar

Hernández-Rodríguez

Suárez-López

et al. 2020

Remexca

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Las condiciones de cultivo y el manejo poscosecha del cilantro y las fresas, propician su contaminación microbiana. Existen diversos procedimientos para desinfectar estos productos, sin embargo, es necesario establecer ventajas y desventajas de su aplicación. Los objetivos de este trabajo fueron comparar la eficacia de algunos tratamientos de desinfección para reducir la carga bacteriana en cilantro y fresa y evaluar las propiedades antioxidantes de dichos alimentos, antes y después de la desinfección. Los tratamientos de desinfección comparados fueron: ozono generado en un aparato electrodoméstico a un flujo de 6.25x10-5 mol min-1 y ozono obtenido en laboratorio mediante una reacción electrolítica a un flujo de 7.75x10-7 mol min-1. Asimismo, se utilizaron desinfectantes comerciales a base de dióxido de cloro, plata coloidal y dos productos obtenidos de extractos cítricos, uno aplicado después de un lavado con detergente (extracto estandarizado de semillas cítricas y glicerina) y el otro utilizado directamente (extracto de semillas cítricas a base de ácidos láctico y ascórbico). Posterior a los tratamientos de desinfección se llevó a cabo el conteo de unidad formadora de colonia y se determinaron los pigmentos característicos de los productos desinfectados, el contenido fenólico total y la capacidad antioxidante por el ensayo FRAP. Los resultados demostraron que el extracto estandarizado de semillas cítricas y glicerina resultó ser más efectivo (p< 0.05) para desinfectar tanto fresas como cilantro, al disminuir en 97 y 99.9% las unidades formadoras de colonias, respectivamente; sin embargo, redujo la concentración de antocianinas en fresas y la capacidad antioxidante en el cilantro.

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Influence of Polar Solutions on the Extraction of Phenolic Compounds from Capulín Fruits (Prunus serotina)

Cited by 7 publications

References 12 publications

Correlation Study of Antioxidant Activity with Phenolic and Flavonoid Compounds in 12 Indonesian Indigenous Herbs

Correlation Study of Antioxidant Activity with Phenolic and Flavonoid Compounds in 12 Indonesian Indigenous Herbs

Total Phenols, Flavonoids and Antioxidant Activity in Annona muricata and Annona purpurea Callus Culture

Eficacia de métodos de desinfección y los efectos sobre las propiedades nutracéuticas en cilantro y fresa

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