Analytical Methods for Determination of Reactive Oxygen Species

Pranczk, Joanna; Jacewicz, Dagmara; Wyrzykowski, Dariusz; Chmurzyński, Lech

doi:10.2174/1573412910666140530221254

Cited by 24 publications

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“…The reaction of VO(IDA)phen with O 2 · – in the presence of nitro blue tetrazolium (NBT) was monitored spectrophotometrically to examine the ability of this complex to scavenge superoxide radicals 42. During the reaction, monoformazan was formed.…”

Section: Resultsmentioning

confidence: 99%

Crystal Structure, Antioxidant Properties and Characteristics in Aqueous Solutions of the Oxidovanadium(IV) Complex [VO(IDA)phen]·2H₂O

et al. 2015

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A new oxidovanadium(IV) complex was prepared by the reaction of vanadyl acetylacetonate [VO(acac)2], iminodiacetic acid (H2IDA) and 1,10‐phenanthroline (phen) in aqueous solutions. The crystal structure of [VO(IDA)phen]·2H2O was determined by X‐ray diffraction crystallographic analysis. The stability of the title compound in aqueous solution was investigated by using potentiometric titration methods. Moreover, the kinetics of substitution reactions of the VO(IDA) binary complex with 1,10‐phenanthroline were investigated in aqueous solutions. These reactions were monitored spectrophotometrically at 249 nm by using stopped‐flow methods. Kinetic measurements were performed in the 293–308 K temperature range, at different concentrations (0.1–0.5 mM) of the binary complex and at a constant concentration of phen (0.05 mmol/L). Moreover, the antioxidant properties of this complex against superoxide radicals were studied by nitrobluetetrazolium (NBT) and cyclic voltammetry (CV) and the antioxidant properties were also investigated against two organic radicals: the 1,1‐diphenyl‐2‐picrylhydrazyl radical (DPPH·) and the 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) radical (ABTS·+) by using spectrophotometric methods. The results of the antioxidant tests show that VO(IDA)phen scavenges superoxide free radicals as well as organic radicals (ABTS·+ and DPPH·).

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Section: Resultsmentioning

confidence: 99%

Crystal Structure, Antioxidant Properties and Characteristics in Aqueous Solutions of the Oxidovanadium(IV) Complex [VO(IDA)phen]·2H₂O

et al. 2015

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“…The ability of VO(IDA)bipy to scavenge superoxide radicals were examined by the NBT test [2,27,38]. The best fitting for the curves based on the results of the NBT experiment was obtained for the equation y = y 0 + AÁe (x/t) .…”

Section: Antioxidant Properties Of Vo(ida)bipy and Vo(ida) Against Sumentioning

confidence: 99%

“…An excess of ROS is a serious threat to the proper functioning of the body and leads to the oxidative stress [1,2]. The role of superoxide dismutase is to remove the excess of superoxide anion.…”

Section: Introductionmentioning

confidence: 99%

Structural, physico-chemical and antioxidant characteristics of 2,2′-bipyridyl(iminodiacetato)oxidovanadium(IV) dihydrate

et al. 2015

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“…However, the intracellular mitochondria also produce oxidative substances through the mitochondrial respiratory chain, namely reactive oxygen species (ROS), including hydrogen peroxide (H 2 O 2 ), oxygen radicals (⋅O 2 − ), and hydroxyl radicals (⋅OH). 25,26 The ROS levels are relatively low and can be promptly cleared by numerous antioxidant enzymes in normal cells. However, they are significantly higher in tumor cells and induce cytotoxicity after exceeding a certain threshold, followed by inhibiting extracellular excretion of drugs through the inhibition of cell mitochondrial function and ATP synthesis, ultimately inducing apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Redox-responsive F127-folate/F127-disulfide bond-D-α-tocopheryl polyethylene glycol 1000 succinate/P123 mixed micelles loaded with paclitaxel for the reversal of multidrug resistance in tumors

Lin¹,

Zhao²,

Liu³

et al. 2018

IJN

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Introduction The development of nanodrug carriers utilizing tumor microenvironment has become a hotspot in reversing multidrug resistance (MDR). Materials and methods This study synthesized a redox-sensitive copolymer, Pluronic F127-disulfide bond- d -α-tocopheryl polyethylene glycol 1000 succinate (FSST), through the connection of the reduction-sensitive disulfide bond between F127 and d -α-tocopheryl polyethylene glycol 1000 succinate. This polymer could induce the elevation of reactive oxygen species (ROS) levels, ultimately resulting in cytotoxicity. Moreover, the redox-responsive mixed micelles, F127-folate (FA)/FSST/P123 (FFSSTP), based on FSST, Pluronic F127-FA, and Pluronic P123, were prepared to load paclitaxel (PTX). Results The in vitro release study demonstrated that FFSSTP/PTX accelerated the PTX release through the breakage of disulfide bond in reductive environment. In cellular experiment, FFSSTP/PTX induced significant apoptosis in PTX-resistant MCF-7/PTX cells through inhibiting adenosine triphosphate (ATP)-binding cassette proteins from pumping out PTX by interfering with the mitochondrial function and ATP synthesis. Furthermore, FFSSTP/PTX induced apoptosis through elevating the intracellular levels of ROS. Conclusion FFSSTP could become a potential carrier for the treatment of MDR tumors.

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Analytical Methods for Determination of Reactive Oxygen Species

Cited by 24 publications

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Crystal Structure, Antioxidant Properties and Characteristics in Aqueous Solutions of the Oxidovanadium(IV) Complex [VO(IDA)phen]·2H₂O

Crystal Structure, Antioxidant Properties and Characteristics in Aqueous Solutions of the Oxidovanadium(IV) Complex [VO(IDA)phen]·2H₂O

Structural, physico-chemical and antioxidant characteristics of 2,2′-bipyridyl(iminodiacetato)oxidovanadium(IV) dihydrate

Redox-responsive F127-folate/F127-disulfide bond-D-α-tocopheryl polyethylene glycol 1000 succinate/P123 mixed micelles loaded with paclitaxel for the reversal of multidrug resistance in tumors

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Analytical Methods for Determination of Reactive Oxygen Species

Cited by 24 publications

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Crystal Structure, Antioxidant Properties and Characteristics in Aqueous Solutions of the Oxidovanadium(IV) Complex [VO(IDA)phen]·2H2O

Crystal Structure, Antioxidant Properties and Characteristics in Aqueous Solutions of the Oxidovanadium(IV) Complex [VO(IDA)phen]·2H2O

Structural, physico-chemical and antioxidant characteristics of 2,2′-bipyridyl(iminodiacetato)oxidovanadium(IV) dihydrate

Redox-responsive F127-folate/F127-disulfide bond-D-&alpha;-tocopheryl polyethylene glycol 1000 succinate/P123 mixed micelles loaded with paclitaxel for the reversal of multidrug resistance in tumors

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Crystal Structure, Antioxidant Properties and Characteristics in Aqueous Solutions of the Oxidovanadium(IV) Complex [VO(IDA)phen]·2H₂O

Crystal Structure, Antioxidant Properties and Characteristics in Aqueous Solutions of the Oxidovanadium(IV) Complex [VO(IDA)phen]·2H₂O

Redox-responsive F127-folate/F127-disulfide bond-D-α-tocopheryl polyethylene glycol 1000 succinate/P123 mixed micelles loaded with paclitaxel for the reversal of multidrug resistance in tumors