Analytical methods based on the light-scattering of plasmonic nanoparticles at the single particle level with dark-field microscopy imaging

Li, Tian; Wu, Xi; Liu, Feng; Li, Na

doi:10.1039/c6an02384c

Cited by 54 publications

(30 citation statements)

References 55 publications

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“…The resonance wavelength is highly sensitive to changes in the local dielectric environment and hence Δλ max shifts can be monitored to detect binding events along with structural changes in biomacromolecular conformation [ 27 ]. For arrays of nanoparticles on a solid support, these measurements can be conducted at the single-particle level by measuring scattering intensity in a dark field illumination setup [ 33 , 34 , 35 ] or, at the ensemble-average level, by measuring changes in extinction intensity (absorption + elastic scattering) in transmission or reflection modes [ 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Probing the Interaction of Dielectric Nanoparticles with Supported Lipid Membrane Coatings on Nanoplasmonic Arrays

Ferhan

Junren

Jackman

et al. 2017

Sensors

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The integration of supported lipid membranes with surface-based nanoplasmonic arrays provides a powerful sensing approach to investigate biointerfacial phenomena at membrane interfaces. While a growing number of lipid vesicles, protein, and nucleic acid systems have been explored with nanoplasmonic sensors, there has been only very limited investigation of the interactions between solution-phase nanomaterials and supported lipid membranes. Herein, we established a surface-based localized surface plasmon resonance (LSPR) sensing platform for probing the interaction of dielectric nanoparticles with supported lipid bilayer (SLB)-coated, plasmonic nanodisk arrays. A key emphasis was placed on controlling membrane functionality by tuning the membrane surface charge vis-à-vis lipid composition. The optical sensing properties of the bare and SLB-coated sensor surfaces were quantitatively compared, and provided an experimental approach to evaluate nanoparticle–membrane interactions across different SLB platforms. While the interaction of negatively-charged silica nanoparticles (SiNPs) with a zwitterionic SLB resulted in monotonic adsorption, a stronger interaction with a positively-charged SLB resulted in adsorption and lipid transfer from the SLB to the SiNP surface, in turn influencing the LSPR measurement responses based on the changing spatial proximity of transferred lipids relative to the sensor surface. Precoating SiNPs with bovine serum albumin (BSA) suppressed lipid transfer, resulting in monotonic adsorption onto both zwitterionic and positively-charged SLBs. Collectively, our findings contribute a quantitative understanding of how supported lipid membrane coatings influence the sensing performance of nanoplasmonic arrays, and demonstrate how the high surface sensitivity of nanoplasmonic sensors is well-suited for detecting the complex interactions between nanoparticles and lipid membranes.

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Section: Introductionmentioning

confidence: 99%

Probing the Interaction of Dielectric Nanoparticles with Supported Lipid Membrane Coatings on Nanoplasmonic Arrays

Ferhan

Junren

Jackman

et al. 2017

Sensors

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“…Due to the localized surface plasmon resonance (LSPR) effect 16 – 18 , individual noble metal nanocrystals scatter light strongly with a distinct color, allowing it to be clearly distinguished by a DFM 19 , 20 . Remarkably, AuNRs and AuNSs can be easily distinguished by a non-scanning DFM owing to the different color of light that they scatter 21 , 22 .…”

Section: Resultsmentioning

confidence: 99%

Microscopic Differentiation of Plasmonic Nanoparticles for the Ratiometric Read-out of Target DNA

Yang

et al. 2017

Sci Rep

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The development of highly sensitive and rapid methods for detecting DNA is of critical importance. Here, we describe a strategy for the digital detection of target DNA at the femto-molar level. Individual DNA molecules were encoded with a single gold nanorod (AuNR), separated and enriched by magnetic immune-separation. The coding gold nanorods were then de-hybridized and dispersed into a gold nanosphere (AuNS) solution at a certain concentration, and both gold nanoparticles were immobilized on glass slides for dark-field microscopic imaging. Using an in-house Matlab program, the concentration of the target DNA was calculated based on the ratio of the coding gold nanorods to gold nanospheres. By combining the coding of individual biomolecules with a single gold nanorod and the use of gold nanospheres as an internal standard, a method for the rapid and accurate digital detection of target DNA to the femto-molar level was developed.

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“…In particular, darkfield microscopy shows suitable performance when the refractive indices of the sample are very close to those of its surroundings, such as in small aquatic organisms and cells, which are hardly detectable with conventional brightfield microscopy. AuNPs scatter strongly on LSPR frequency so that treated specimens can be visualized as bright spots with the characteristic plasmonic color of dark field microscopy [ 127 ]. Moreover, owing to the high resistance to photobleaching, AuNP is widely applied as long-time observation imaging probe for biological samples with high resolution and sensitivity [ 128 ].…”

Section: Photoimagingmentioning

confidence: 99%

State of the Art Biocompatible Gold Nanoparticles for Cancer Theragnosis

et al. 2020

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Research on cancer theragnosis with gold nanoparticles (AuNPs) has rapidly increased, as AuNPs have many useful characteristics for various biomedical applications, such as biocompatibility, tunable optical properties, enhanced permeability and retention (EPR), localized surface plasmon resonance (LSPR), photothermal properties, and surface enhanced Raman scattering (SERS). AuNPs have been widely utilized in cancer theragnosis, including phototherapy and photoimaging, owing to their enhanced solubility, stability, biofunctionality, cancer targetability, and biocompatibility. In this review, specific characteristics and recent modifications of AuNPs over the past decade are discussed, as well as their application in cancer theragnostics and future perspectives. In the future, AuNP-based cancer theragnosis is expected to facilitate the development of innovative and novel strategies for cancer therapy.

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Analytical methods based on the light-scattering of plasmonic nanoparticles at the single particle level with dark-field microscopy imaging

Cited by 54 publications

References 55 publications

Probing the Interaction of Dielectric Nanoparticles with Supported Lipid Membrane Coatings on Nanoplasmonic Arrays

Probing the Interaction of Dielectric Nanoparticles with Supported Lipid Membrane Coatings on Nanoplasmonic Arrays

Microscopic Differentiation of Plasmonic Nanoparticles for the Ratiometric Read-out of Target DNA

State of the Art Biocompatible Gold Nanoparticles for Cancer Theragnosis

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