Analytical Method Validation of Quercetin in Changnyeong Onion Extract as a Functional Ingredient for Functional Health Food

전선영,; 정은정,; 백정화,; 차용준,

doi:10.3746/jkfn.2011.40.4.565

Cited by 8 publications

(4 citation statements)

References 8 publications

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“…Forest plant resources, including fragrance substances from plants, animals, and microorganisms, are valuable for enhancing taste, color, and aroma. Phytochemicals produced by plants during growth, mainly phenolic compounds, play a crucial role as major contributors to antioxidant activity, as emphasized in numerous studies [1,8]. Women undergoing menopause are prescribed hormone replacement therapy to relieve these symptoms; however, concerns about its side effects have led many women to choose non-hormonal therapies [26,28].…”

Section: Discussionmentioning

confidence: 99%

“…Some of these phytochemicals exhibit potent antioxidant activities [6]. Numerous studies have emphasized the role of phenolic compounds in plants as significant contributors to antioxidant activity [7][8][9]. Among the phytochemicals with potent antioxidant effects, caffeic acid (1), p-coumaric acid (2), and quercetin (3) are present in various plants.…”

Section: Introductionmentioning

confidence: 99%

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Estrogen-like Cell Proliferation Abilities of Korea Forest Plant Resources on MCF-7 Cells and Analysis of Active Compounds

Cho,

Tran,

Ann

et al. 2024

Forests

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This study conducted a comprehensive analysis of functional substances and explored their biological activities using colorimetric and chromatographic techniques to identify high-value materials from 14 species of forest plants native to Korea. Comparative analysis between plant species included calculating the total polyphenol and flavonoid contents and qualitative and quantitative analysis of the phytochemical compounds caffeic acid (1), p-coumaric acid (2), and quercetin (3) using HPLC, and antioxidant activity tests (DPPH and ABTS+) and menopausal symptom relief tests (E-screen assay) to investigate their biological activities. The results highlighted Cercidiphyllum japonicum (FR 4), Aruncus dioicus (FR 6), and Pseudocydonia sinensis (FR 8) as excellent forest plant resource materials among the 14 forest plant resources. Notably, FR 4 exhibited significant activity in the E-screen assay. On the basis of these findings, we propose the use of FR 4 as a valuable material. Further analysis identified the phytochemical compounds maltol (4), chlorogenic acid (5), ellagic acid (6), and quercitrin (7) in FR 4 and analyzed their biological activities. Compounds 2–6 found in FR 4 were confirmed to possess strong antioxidant activity, and an E-screen assay revealed an excellent cell proliferation rate for quercitrin (7). This suggests that quercitrin (7) in FR 4 may be a significant indicator of the alleviation of menopausal symptoms.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Estrogen-like Cell Proliferation Abilities of Korea Forest Plant Resources on MCF-7 Cells and Analysis of Active Compounds

Cho,

Tran,

Ann

et al. 2024

Forests

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“…In particular, standardization and specification of raw materials are important in the development and production of healthy foods using medicinal herbs, and quality control methods using indicator components' content to scientifically prove the functionality and safety of raw materials (Kim et al, 2012). Such quality control methods are the most common way to minimize differences in indicator components present in raw materials to reach in final products, thus maintaining the desired components during the manufacturing process (Jeon et al, 2011). Indicator components are selected for standardization of raw materials, taking into consideration the availability, specificity, representativeness, safety, ease of analysis technology, and reliability (Ahn et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Establishment of simultaneous analysis validation for Perilla frutescens var. acuta extract

JEON,

CHOI,

Yim

2023

Food Sci. Technol (Campinas)

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In this study, the UV spectra maximum absorption wavelengths and molecular weights of major compounds 1–3 were identified in Perilla frutescens var. acuta extract using high-performance liquid chromatography (HPLC)-DAD and HPLC-MS/MS. Luteolin-7-O-diglucuronide (1), scutellarin (2), and rosmarinic acid (3) were identified, and simultaneous analysis validation was performed for quantification using HPLC-DAD. All linearities showed R2 values of 0.9993 or higher, and the limits of detection and quantification were measured in the ranges of 4.4802–5.4133 and 13.5764–16.4038 µg/mL, respectively. The relative standard deviation (RSD) values for accuracy ranged from 0.09 to 0.89%, and the recovery rates were in the range of 99.36–101.96%. The precision RSD values within the laboratory were in the range of 0.05–1.04%, and the RSD values for repeatability evaluation were below the guideline criteria of 2%. The content change of the three marker compounds within 24 h was 0.36–0.69%, confirming the chemical stability of the extract within 24 h, which was below the guideline criteria of 2.0% established by the Ministry of Food and Drug Safety guidelines. The content analysis of the extract according to ethanol concentration showed that luteolin-7-O-diglucuronide had the highest content of 302.22 µg/mL in the 0% ethanol extract, while scutellarin and rosmarinic acid showed the highest contents of 103.61 and 118.77 µg/mL, respectively, in the 60% ethanol extract.

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“…In addition, there may be differences in the content of the major constituents in the process of heating and extracting them. Thus, it is essential to check the content of the surface ingredients and ensure that the content is validated and an analysis method considered to be accurate is applied to make the analysis more feasible and reliable [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Validation of a Quantification Method for Curcumin Derivatives and Their Hepatoprotective Effects on Nonalcoholic Fatty Liver Disease

Lee

et al. 2022

CIMB

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Curcumin (CM), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) are major curcumin derivatives found in the rhizome of turmeric (Curcuma longa L.), and have yielded impressive properties to halt various diseases. In the present study, we carried out a method validation for curcumin derivatives and analyzed the contents simultaneously using HPLC with UV detection. For validation, HPLC was used to estimate linearity, range, specificity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). Results showed a high linearity of the calibration curve, with a coefficient of correlation (R2) for CM, DMC, and BDMC of 0.9999, 0.9999, and 0.9997, respectively. The LOD values for CM, DMC, and BDMC were 1.16, 1.03, and 2.53 ng/μL and LOQ values were 3.50, 3.11, and 7.67 ng/μL, respectively. Moreover, to evaluate the ability of curcumin derivatives to reduce liver lipogenesis and compare curcumin derivatives’ therapeutic effects, a HepG2 cell model was established to analyze their hepatoprotective properties. Regarding the in vivo study, we investigated the effect of DMC, CM, and BDMC on nonalcoholic fatty liver disease (NAFLD) caused by a methionine choline deficient (MCD)-diet in the C57BL/6J mice model. From the in vitro and in vivo results, curcumin derivatives alleviated MCD-diet-induced lipid accumulation as well as high triglyceride (TG) and total cholesterol (TC) levels, and the protein and gene expression of the transcription factors related to liver adipogenesis were suppressed. Furthermore, in MCD-diet mice, curcumin derivatives suppressed the upregulation of toll-like receptors (TLRs) and the production of pro-inflammatory cytokines. In conclusion, our findings indicated that all of the three curcuminoids exerted a hepatoprotective effect in the HepG2 cell model and the MCD-diet-induced NAFLD model, suggesting a potential for curcuminoids derived from turmeric as novel therapeutic agents for NAFLD.

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Analytical Method Validation of Quercetin in Changnyeong Onion Extract as a Functional Ingredient for Functional Health Food

Cited by 8 publications

References 8 publications

Estrogen-like Cell Proliferation Abilities of Korea Forest Plant Resources on MCF-7 Cells and Analysis of Active Compounds

Estrogen-like Cell Proliferation Abilities of Korea Forest Plant Resources on MCF-7 Cells and Analysis of Active Compounds

Establishment of simultaneous analysis validation for Perilla frutescens var. acuta extract

Validation of a Quantification Method for Curcumin Derivatives and Their Hepatoprotective Effects on Nonalcoholic Fatty Liver Disease

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