Analytical Method Validation of Icp-Aes for Analysis of Cadmium, Chromium, Cuprum, Mangan and Nickel in Milk

Abstract

Objective: The aim of this research was to validate inductively coupled plasma-atomic emission spectroscopy (ICP-AES) for quantitative analysis of cadmium (Cd), chromium (Cr), cuprum (Cu), mangan (Mn) and nickel (Ni) in milk products.
 Methods: The heavy metals in milk were determined using ICP-AES at optimized wavelength. The method was validated by assessing several validation parameters which included linearity and range, accuracy, precision and sensitivity expressed by the limit of detection and limit of quantification. The validated method was then used for the analysis of milks commercially available.
 Results: ICP-AES for determination of Cd, Cr, Cu, Mn, and Ni was linear over a certain concentration range with a coefficient correlation value of>0.997. The limit of quantification values of Cd, Cr, Cu, Mn, and Ni were 0.0047; 0.0050; 0.0066; 0.0061; and 0.0169 µg/ml, respectively. The precision of analytical method exhibited relative standard deviation (RSD) values of 3.18%; 4.17%; 3.05%; 2.93%; and 4.47% during repeatability test and 5.28%; 5.06%; 3.67%; 3.67%; and 11.17% during intermediate precision of Cd, Cr, Cu, Mn, and Ni respectively. The recoveries of these metals assessed using standard addition method were 92.25; 90.88; 102.87; 94.50; and 86.85%, respectively.
 Conclusion: ICP-AES offered a reliable and fast method for the determination of heavy metals in milk products. The developed method could be proposed as an official method for determination of heavy metals in milk products.

Recent Advances in Detection and Removal of Heavy Metals from Contaminated Water

“…The ICP-AES was employed on milk products, and multiple heavy metals like cadmium, chromium, copper, manganese, and nickel were quantitatively determined with high precision [35]. The method is effective due to its precision, accuracy, high sensitivity, and remarkable selectivity but the spectral interferences are still a severe risk to sample analysis.…”

Validation of the method for determining toxic elements, micro- and macroelements in biological samples using atomic emission inductively coupled plasma (ICP OES)

“…Оптичний спектр з можливістю аксіального та радіального перегляду дозволяє одночасно визначати велику кількість елементів в діапазоні від 165 до 800 нм (Ipeaiyeda & Ayoade, 2017). Тому для якісного, адекватного встановлення точного діагнозу (отруєння токсичними елементами, мікро-, макроелементози) важливо мати точний кількісний метод визначення елементів в індикаторних біосубстратах тварин (Burke et al, 2016;Alexander & Rohman, 2019).…”