Analytical method for ubiquinone-9 and ubiquinone-10 in rat tissues by liquid chromatography/turbo ion spray tandem mass spectrometry with 1-alkylamine as an additive to the mobile phase

A rapid and sensitive LC-MS/MS method for determination of coenzyme Q10 in tobacco (Nicotiana tabacum L.) leaves

“…In particular, it is very effective in the analysis of CoQ 10 in complex samples because of its low detection limit and high sensitivity. Teshima et al established a sensitive and simple quantification method for ubiquinone-9 (CoQ 9 ) and ubiquinone-10 (CoQ 10 ) in rat thigh muscle and heart using LC-MS/MS [15]. Hansen et al developed two new sensitive and selective LC-MS methods for the quantification of the total coenzyme Q 10 concentration in human blood serum and both methods were shown to be more selective and to have comparable or better sensitivity than the HPLC-ECD method [16].…”

Analysis of coenzyme Q10 in lymphocytes by HPLC–MS/MS

“…Dwell time for each transition was 200 ms and the run-time was 16 min. Analytical conditions of the HPLC-MS/MS were those described by Teshima and Kondo [9], with some modifications. Briefly, the mobile phase was composed of 50% methanol with methylamine 5 mM, 45% 2-propanol and 5% water acidified with formic acid (0.5 mL/L), at a flow rate of 0.2 mL/min and isocratic conditions.…”

“…Immediate diagnosis leads to better prevention and correction of the clinical symptoms. Current methods for the determination of CoQ 10 content are based on reversed phase high performance liquid chromatography (HPLC) with ultraviolet detection [4,5], electrochemical detection [6][7][8] or tandem mass spectrometry detection (MS/MS) [9,10]. Currently, it is under discussion which is the best biological tissue to detect CoQ 10 defects.…”

Determination by UPLC/MS-MS of Coenzyme Q10 (CoQ10) in Plasma of Healthy Volunteers before and after Oral Intake of Food Supplements Containing CoQ10

“…For these reasons in the last few years, the number of CoQ10 dietary supplements has increased rapidly. Several analytical methods have been developed for the determination of CoQ10 content, based on spectrophotometric, colorimetric [27], electrochemical [28,29] and electrophoretic techniques [30], but the method of choice is reversed phase high performance liquid chromatography (LC) with ultraviolet detection [31,32], electrochemical detection [33][34][35] or tandem mass spectrometry detection (MS/MS) [36,37]. The more recent reports deals with the development of miniaturized LC/UV systems [38] and the demonstration of the effect of CoQ10 in the reduction of the oxidative stress and of the inflammatory markers [39,40].…”