A rapid and sensitive LC-MS/MS method for determination of coenzyme Q10 in tobacco (Nicotiana tabacum L.) leaves

Abstract

A rapid and sensitive liquid chromatography-tandem mass spectrometry method with multiple reaction monitoring has been proposed for the analysis of coenzyme Q10 in (CoQ10) tobacco leaves. The method used electrospray ionization with detection in positive ion mode. Sample pretreatment involved ultrasonic extraction of fresh tobacco leaves with anhydrous ethanol for 15 min and followed by extraction of the supernatant with hexane. The separation of CoQ10 was performed on a Symmetry Shield RP18 column with a mixture of acetonitrile and isopropanol (8:7, v/v) containing 0.5% formic acid as mobile phase. Quantification of CoQ10 was performed by the standard addition method. The limit of detection and limit of quantitation of CoQ10 were, respectively, 1.2 ng/mL (S/N = 3) and 4.0 ng/mL (S/N = 10). The relative standard deviations of peak area were 0.91% and 1.21% for intra-day and inter-day, respectively. The recoveries of CoQ10 ranged from 98.2 to 99.3% and the corresponding RSDs were less than 2.4%. Analysis took 5 min, making the method suitable for rapid determination of CoQ10 in tobacco leaves. The proposed method has been successfully applied to the analysis of CoQ10 in the leaves from eight varieties of tobacco.

The hypoxia‐induced dehydrogenase HorA is required for coenzyme Q10 biosynthesis, azole sensitivity and virulence of Aspergillus fumigatus

“…For HPLC analysis, an ACCUCORE RP-MS 2.6 mm 150 3 4.6 mm column (Thermo Fisher Scientific, Dreieich, Germany) was applied. The mobile phase consisted of an isocratic gradient of acetonitrile and isopropanol (8:7, v/v) containing 0.5% (v/v) formic acid (Zu et al, 2006). The run was 5 min in length and quantification was completed using the mass spectrometer in positive mode.…”

COENZYME Q10 TREATMENTS INFLUENCE THE LIFESPAN AND KEY BIOCHEMICAL RESISTANCE SYSTEMS IN THE HONEYBEE, Apis mellifera

“…CoQ10, a lipid‐soluble endogenous provitamin found naturally in the mitochondria, is found in many organisms (Jiang et al., ; Zu et al., ). For several years, the study of CoQ10 in foodstuffs and animal tissue has attracted special attention because of its crucial roles in many biochemical pathways and important health functions.…”

Биофармацевтический анализ коэнзима Q₁₀ (убидекаренона)

“…Ïðè àíàëèçå CoQ 10 â ðàçëè÷íûõ áèîëîãè÷åñêèõ îáúåêòàõ äàííûå ïðåèìóùåñòâà ñóùåñòâåííû. Ðàáîòà [39] ïîñâÿùåíà îïðåäåëåíèþ CoQ 10 ìåòîäîì ÂÝAEÕ ñ èñïîëüçîâàíèåì ðàçëè÷íûõ äåòåêòîðîâ: ýëåêòðîõèìè÷åñêîãî è ìàññ-ñïåêòðîìåòðè÷åñêîãî, îñíàùåííîãî èîííîé ëîâóøêîé.  êà÷åñòâå èñòî÷íèêà èîíèçèðóþùåãî èçëó÷åíèÿ èñïîëüçîâàëè ýëåêòðîñïðåé è õèìè÷åñêóþ èîíèçàöèþ ïðè àòìîñôåðíîì äàâëåíèè.…”