Analytical Method for the Validation of Three Polyphenols as a Marker Compound for the Standardization of Solidago virgaurea subsp. gigantea Extracts and Antiadipogenesis of Harvesting Time and Location

Abstract

Protocatechuic acid (PC), chlorogenic acid (CA), and kaempferol-3-O-rutinoside (K-O-R), isolated from the Solidago virgaurea subsp. gigantea (SV) extract, were quickly and efficiently separated using HPLC. Our chromatographic method was found to effectively separate PC, CA, and K-O-R at retention times of 5.36, 8.22, and 17.04 min, respectively. Linearity of PC, CA, and K-O-R was found to be in the range of 4.85–485.00, 47.5–1900.00, and 8.50–850.00 μg/ml. Recoveries ranged between 101.32 and 103.30%, 95.82 and 100.25%, and 96.18 and 99.37%, for PC, CA, and K-O-R, respectively. The antiadipogenesis activity of SV extracts collected from five different months and from seven different regions was evaluated using an Oil Red O staining assay in 3T3-L1 cells. Extract from SV collected in April from the Ulleung Island produced over 106.89% inhibition of adipogenesis without cytotoxicity at 50 μg/ml. This extract had a high amount of PC and K-O-R. The developed HPLC method was found to be fast, accurate, precise, and reproducible and could be applied to qualitative and quantitative analysis of three bioactive compounds in SV extracts. The SV extract collected in April from Ulleung Island can be used as a functional food ingredient preventing obesity.

Development of analysis method of andrographolide from Andrographis paniculata using UPLC-PDA

“…Validation of the proposed method was performed which includes Specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantitation (LOQ) (EMA, 2011;Hwang et al, 2017;Lee et al, 2021;LoBrutto and Patel, 2007;Syukri et al, 2015) 2.8. System suitability Six replicate injections of the sample sennoside B at a concentration of 125 μg/mL in the methanolic solution was simultaneously analysed.…”

“…In analytical chemistry, specificity pertains to the ability of a method to accurately quantify individual components in a complex mixture. In this study, specificity was evaluated by comparing the chromatograms and PDA spectral patterns of standard solutions, a blank solution consisting of MeOH, and the A. paniculata extract (Hwang et al, 2017;Lee et al, 2021).…”

“…The limits of detection (LODs) and quantification (LOQs) were determined as the minimum concentrations of standard solutions or the lowest spiked concentrations in fortified samples that generated a signal-to-noise ratio of 3 and 10, respectively. The detection and quantification limits were calculated using the following formula (Hwang et al, 2017;Syukri et al, 2015).…”

Development and validation of analysis method for sennoside B in Cassia angustifolia using UPLC-MRM/MS

“…The term specificity in analytical method refers to its ability to accurately measure individual components in a complex mixture. In this study, the specificity of the UPLC-MS/MS method was evaluated by comparing the chromatograms and multiple reaction monitoring (MRM) spectral patterns of standard solutions, blank solutions (methanol), and the C. angustifolia extract (Hwang et al, 2017;Lee et al, 2021).…”

“…were determined as the lowest concentrations of standard solutions or the lowest spiked concentrations in fortified samples that produce a signal-to-noise ratio of 3 and 10, respectively. The calculation of the LOD and LOQ was performed using the following formula (Hwang et al, 2017;Syukri et al, 2015).…”