Analytical features of particle counting sensor based on plasmon assisted microscopy of nano objects

Application of surface plasmon resonance imaging technique for the detection of single spherical biological submicrometer particles

“…Thus, SPR-based sensor appears to be especially useful in research areas, when precise quantifications of intact biological particles are crucial [8]. In addition, it is noteworthy that despite the fact that low concentration samples were not used in the current research work, the detection limit of the SPR-based sensor may be significantly better compared to the current study as discussed in the previous work [6].…”

“…Only recent groundbreaking studies performed in independent working groups helped in realizing the potential of SPR-based sensors in studies of biological submicrometer and nanoparticles [4][5][6][7]. Zybin and colleagues used SPR-based ''microscopy'' (also called ''SPR nanoscopy'') to detect the binding of individual polystyrene particles of different sizes (minimal used size was 40 nm diameter) to the functionalized sensor surface [6].…”

“…Zybin and colleagues used SPR-based ''microscopy'' (also called ''SPR nanoscopy'') to detect the binding of individual polystyrene particles of different sizes (minimal used size was 40 nm diameter) to the functionalized sensor surface [6]. The same working group detected the binding of single human immunodeficiency virus (HIV)-VLPs (around 100-140 nm diameter) to the antibody-coated sensor surface [6]. Wang and colleagues demonstrated the ability of SPR-based sensors to detect single influenza A viral particles in solutions [5].…”

“…Thus, with this method instant information about the existence of target biological submicrometer particles in analyzed samples can be obtained. Besides this characteristic, the SPRi method has another crucial advantage: in comparison with culture assays or other direct methods: the SPR-based assay can be performed within minutes and only in the case of very low concentrations of viruses requires hours for viral detection [6]. However, direct methods often require long turnaround time (days) and intensive labor procedures.…”

Surface Plasmon Resonance Imaging Microscopy of Liposomes and Liposome-Encapsulated Gold Nanoparticles

“…This can be achieved by using an objective with a high numerical aperture (NA) that possess multiple advantages in comparison to the normal SPR prism-coupled SPR microscopy that may suffer from distortion at the long working distances (see e.g. [4][5][6] ). The high NA objective outputs collimated light that is set to an incident angle when the light beam is focused to the offset of the objective's back focal plane (BFP).…”