Analytical Comparison between Microhematocrit and Automated Methods for Packed Cell Volume (PCV)

Audu, Stephen; Ubwa, Simon Terver; Igbum, Ogbene Gillian; Hati, Stephen S.; Alex, NwannadiI.

doi:10.15226/2639-7986/2/1/00105

Cited by 5 publications

(2 citation statements)

References 6 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The blood samples preserved in EDTA were mixed using a blood mixer with the capillary tube being sealed with plasticine and centrifuged in a microhematocrit centrifuge at 1,000 rpm for 5 min at 4 °C. The packed cell volume (PCV) was read using an HC 702 hematocrit scale as described by Audu et al (2017) . The blood samples in the plain tubes were left to coagulate and then centrifuged at 3,500 rpm for 15 min to obtain clear sera.…”

Section: Data Collectionmentioning

confidence: 99%

Effect of feed supplementation of Dacryodes edulis parts’ powder as prebiotic on the growth traits, ceca microbiota and blood parameters of local chickens

Tangomo¹,

Keambou

Gicheha

et al. 2020

Translational Animal Science

View full text Add to dashboard Cite

This study aimed at testing the efficacy and safety of Dacryodes edulis plant parts in diets fed to chicken. The plant has potential for use as a natural prebiotic to substitute the conventionally used antibiotic growth promoters in poultry production. Phytochemical analyses of the plant leaves, stem, and bark combination (stembark) and seed powders from the D. edulis were carried out. The powder from the three D. edulis plant parts were used as supplement in formulating six experimental diets tested in this study. The diets were TL0Ed (0.5% leaves powder), TL1Ed (1.0% leaves powder), TB0Ed (0.5% stembark powder), TB1Ed (1.0% stembark powder), TS0Ed (0.5% seeds powder), and TS1Ed (1.0% seeds powder). Besides, a positive (T+ positive control; 0.5-g oxytetracycline as recommended by the manufacturer) and a negative (T- negative control; having no commercial antibiotic and no plant supplement) diets were prepared for comparison purposes. The diets were fed to a total of 288 dual-purpose chicken for a period of 14 weeks. The chicken growth and body composition characteristics, blood chemistry, and microbiota count were collected and used as indicators of the plant parts efficacy and safety. The analysis of the D. edulis plant parts significantly differed (P ≤ 0.05) in their phytochemical contents. The initial body weight and feed conversion efficiency ratios were not significantly different (P ≥ 0.05) between and among treatment groups. However, significant differences (P ≤ 0.05) were detected in the feed intake and body weight gain at eighth week. Live weight at eighth week was significantly different (P ≤ 0.05) with its values ranging between 503.32 and 614.93 g for treatments TL1Ed and TNeg-, respectively. The dietary treatment of D. edulis leaves, stembark, and seed powder at the two inclusion levels significantly (P ≤ 0.05) decreased the colonies forming unit of Escherichia coli and Salmonella sp. as compared with negative control treatment in the eighth week phase. The level of glucose, total cholesterol, triglycerides, aspartate aminotransferase, alanine amino transferase, alkaline phosphatase, and the packed cell volume did not differ significantly (P ≥ 0.05) between and among dietary D. edulis treatments. The findings from this research provide crucial information on the efficacy and safety of D. edulis plant parts. This is an important step in testing the potential of the plant in use as a prebiotic in chicken feeds production.

show abstract

Section: Data Collectionmentioning

confidence: 99%

Effect of feed supplementation of Dacryodes edulis parts’ powder as prebiotic on the growth traits, ceca microbiota and blood parameters of local chickens

Tangomo¹,

Keambou

Gicheha

et al. 2020

Translational Animal Science

View full text Add to dashboard Cite

show abstract

“…The alternative approach consists of a manual Hct determination, which involves a specialized micro-capillary rotor (such as a CritSpin TM Microhematocrit Centrifuge) that is also quite expensive. In a clinical setting, the quality of the health care provided to patients is dependent on the accuracy of clinical results; therefore, affordable alternatives for low-income clinics are beneficial for not just the medical personnel but also the patients receiving treatment (Audu et al, 2017). Efficient, cost-effective hematologic instrumentation is essential to minimize errors and ensure reliable results.…”

Section: Introductionmentioning

confidence: 99%

Affordable Method for Hematocrit Determination in Murine Models

Branfield,

Washington,

Manfredi

2024

Current Protocols

View full text Add to dashboard Cite

Hematocrit (Hct) is a powerful tool often used in a clinical setting for the diagnosis of blood conditions such as anemia. It is also used in the research field as a hematological parameter in both human and mouse models. Measuring Hct, however, involves the use of expensive standardized equipment (such as a CritSpin™ Microhematocrit Centrifuge). Here, we describe a novel, simple, and affordable method to determine the Hct in untreated wild‐type (WT) mice and phenylhydrazine (PHZ)‐induced anemic mice with reasonable accuracy, using a benchtop centrifuge commonly available in laboratories. Hct of murine samples processed with a benchtop centrifuge, when compared to the standardized method CritSpin™, showed comparable results. This approach for determining Hct of murine can prove useful to research laboratories that cannot afford specialized equipment for Hct studies. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Affordable Method for Hematocrit Determination in Murine ModelsBasic Protocol 2: Murine Sample ValidationSupport Protocol: Phenylhydrazine‐induced anemia in wild‐type (WT) mice

show abstract

Influence of the physiological stage of Blackbelly sheep on immunological behaviour against gastrointestinal nematodes

González-Gardúño

Gives

Arellano

et al. 2018

Experimental Parasitology

View full text Add to dashboard Cite

Analytical Comparison between Microhematocrit and Automated Methods for Packed Cell Volume (PCV)

Abstract: Warfarin related nephropathy: a case report from a tertiary hospital of north India and review of literature

Cited by 5 publications

References 6 publications

Effect of feed supplementation of Dacryodes edulis parts’ powder as prebiotic on the growth traits, ceca microbiota and blood parameters of local chickens

Effect of feed supplementation of Dacryodes edulis parts’ powder as prebiotic on the growth traits, ceca microbiota and blood parameters of local chickens

Affordable Method for Hematocrit Determination in Murine Models

Influence of the physiological stage of Blackbelly sheep on immunological behaviour against gastrointestinal nematodes

Contact Info

Product

Resources

About