Analysis on the interaction between post-spliced introns and corresponding protein coding sequences in ribosomal protein genes

Zhao, Xiaoqing; Li, Hong; Bao, Tonglaga

doi:10.1016/j.jtbi.2013.03.002

Cited by 9 publications

(4 citation statements)

References 39 publications

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“…Previous studies have shown that exons directly encode proteins and that introns influence RNA synthesis during transcription ( Mattick and Gagen, 2001 ). Moreover, the postspliced introns interact with the corresponding coding sequences and play an important role in regulating mRNA transport and gene expression ( Le Hir et al., 2003 ; Zhao et al., 2013 ; Bo et al., 2019 ). Therefore, the evolution and functional role of FLS introns remain to be studied in the future.…”

Section: Discussionmentioning

confidence: 99%

GbFLSa overexpression negatively regulates proanthocyanin biosynthesis

Guo

Wu²,

Wang³

et al. 2023

Front. Plant Sci.

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Flavonoids are important secondary metabolites with extensive pharmacological functions. Ginkgo biloba L. (ginkgo) has attracted extensive attention because of its high flavonoid medicinal value. However, little is understood about ginkgo flavonol biosynthesis. Herein, we cloned the full-length gingko GbFLSa gene (1314 bp), which encodes a 363 amino acid protein that has a typical 2-oxoglutarate (2OG)-Fe(II) oxygenase region. Recombinant GbFLSa protein with a molecular mass of 41 kDa was expressed in Escherichia coli BL21(DE3). The protein was localized to the cytoplasm. Moreover, proanthocyanins, including catechin, epicatechin, epigallocatechin and gallocatechin, were significantly less abundant in transgenic poplar than in nontransgenic (CK) plants. In addition, dihydroflavonol 4-reductase, anthocyanidin synthase and leucoanthocyanidin reductase expression levels were significantly lower than those of their CK counterparts. GbFLSa thus encodes a functional protein that might negatively regulate proanthocyanin biosynthesis. This study helps elucidate the role of GbFLSa in plant metabolism and the potential molecular mechanism of flavonoid biosynthesis.

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Section: Discussionmentioning

confidence: 99%

GbFLSa overexpression negatively regulates proanthocyanin biosynthesis

Guo

Wu²,

Wang³

et al. 2023

Front. Plant Sci.

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“…Previous studies have shown that exons directly encode proteins and that introns affect RNA synthesis during transcription [ 39 , 40 ]. In addition, an interaction occurs between introns and the corresponding coding sequences after shearing, which plays an important role in the regulation of mRNA transport and gene expression [ 41 , 42 , 43 ]. Hence, the evolutionary and functional roles of the GbF3′5′H1 intron remain to be studied.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of GbF3′5′H1 Provides a Potential to Improve the Content of Epicatechin and Gallocatechin

Wang

Yue

et al. 2020

Molecules

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The flavonoids in Ginkgo biloba L. (ginkgo) have important medicinal uses due to their antioxidant, antitumor, and blood circulation-promoting effects. However, the genetic mechanisms underlying flavonoid biosynthesis in ginkgo remain elusive. Flavonoid 3′, 5′-hydroxylase (F3′5′H) is an important enzyme in flavonoid synthesis. We detected a novel differentially expressed GbF3′5′H1 gene homologous to the F3′5′H enzyme involved in the flavonoid synthesis pathway through transcriptome sequencing. In this study, we characterized this gene, performed an expression analysis, and heterologously overexpressed GbF3′5′H1 in Populus. Our results showed that GbF3′5′H1 is abundant in the leaf and highly expressed during April. We also found four metabolites closely related to flavonoid biosynthesis. Importantly, the contents of 4′,5-dihydroxy-7-glucosyloxyflavanone, epicatechin, and gallocatechin were significantly higher in transgenic plants than in nontransgenic plants. Our findings revealed that the GbF3′5′H1 gene functions in the biosynthesis of flavonoid-related metabolites, suggesting that GbF3′5′H1 represents a prime candidate for future studies (e.g., gene-editing) aiming to optimize ginkgo flavonoid production, especially that of flavan-3-ols.

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“…As a ribosomal protein, RPL39-L can influence the synthesis of a subset of proteins (Petrov et al, 2004;Zhao et al, 2013). The altered translational profile may enhance the overall fitness of the cells and increase the tolerance of the cells and the organisms against harmful chemicals such as amycin (Lippert et al, 2014;Maeda et al, 2014;Hong et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Influence of Ribosomal Protein L39-L in the Drug Resistance Mechanisms of Lacrimal Gland Adenoid Cystic Carcinoma Cells

Ding²,

Wang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: Cancer constitutes a key pressure on public health regardless of the economy state in different countries. As a kind of highly malignant epithelial tumor, lacrimal gland adenoid cystic carcinoma can occur in any part of the body, such as salivary gland, submandibular gland, trachea, lung, breast, skin and lacrimal gland. Chemotherapy is one of the key treatment techniques, but drug resistance, especially MDR, seriously blunts its effects. As an element of the 60S large ribosomal subunit, the ribosomal protein L39-L gene appears to be documented specifically in the human testis and many human cancer samples of different origins.

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Analysis on the interaction between post-spliced introns and corresponding protein coding sequences in ribosomal protein genes

Cited by 9 publications

References 39 publications

GbFLSa overexpression negatively regulates proanthocyanin biosynthesis

GbFLSa overexpression negatively regulates proanthocyanin biosynthesis

Overexpression of GbF3′5′H1 Provides a Potential to Improve the Content of Epicatechin and Gallocatechin

Influence of Ribosomal Protein L39-L in the Drug Resistance Mechanisms of Lacrimal Gland Adenoid Cystic Carcinoma Cells

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