Analysis on residues of estrogens, gestagens and androgens in kidney fat and meat with gas chromatography–tandem mass spectrometry

Impens, Sandra; Wasch, Katia De; Cornelis, M; Brabander, H.F. De

doi:10.1016/s0021-9673(02)00313-8

Cited by 71 publications

(43 citation statements)

References 13 publications

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“…Because MRLs (maximal residue limits) or MRPLs (minimum required performance limits) the progestogens residue in animal food were as low as 1 to 10 μg/kg (Impens et al, 2003;Giannetti et al, 2005), complex and cost-consuming sample clean-up methods have to be adapted for animal fat and meat specimen. Some sample pretreatment and clean-up methods for progestogens in fat and meat have been established including combination of matrix solid phase dispersion and solid phase extraction (Rosen et al, 1994), accelerated solvent extraction and supercritical fl uid extraction for kidney fat (Stolker et al, 2002;Hooijerink et al, 2003) and most of them are solvent extraction and solid phase extraction method (Hageleit et al, 2001;Impens et al, 2002;Giannetti et al, 2005) for kidney fat or meat. Feed containing below 10 μg/kg progestogens is usually ineffective for bringing drug residue in animal tissues, because most of progestogens will be metabolized.…”

Section: Results and Disscusionmentioning

confidence: 99%

A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) method and validation for progestogen multi-residues in feed

Peng

Chen²,

Chen

et al. 2008

J. Anim. Feed Sci.

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Some progestogens as anabolic steroids can be illegally used for increasing the effi ciency of animal food production. The aim of the study was to develop convenient screening method against progestogens in feed. A sensitive enzyme-linked immunosorbent assay (ELISA) method was developed and optimized. The ELISA method is class-specifi c for four relative progestogens and the IC 50 of the method was 3.0 μg/l for MPA. A simple feed pre-treatment method was developed for analysis progestogen residues in feed. The limits of detection (LOD) were 2.0 μg/kg for MPA, 5.0 μg/kg for CMA, 4.6 μg/kg for MEGA and 3.9 μg/kg for HPA in the feed. The recovery was between 72.1 and 95.4% and the coeffi cients of variation (CV) were 7.2 to 15.8%. The results have showed the simple sample pre-treatment method was suitable for progestogen multi-residues rapid analysis in feed.

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Section: Results and Disscusionmentioning

confidence: 99%

A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) method and validation for progestogen multi-residues in feed

Peng

Chen²,

Chen

et al. 2008

J. Anim. Feed Sci.

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“…Trenbolone compound is difficult to analyse by gas chromatography. The difficulty in obtaining derivative stems comes from the structure of a molecule containing three double bonds, and the thermal instability in the gas chromatograph (11,21). Four most commonly used derivatisation reagents and approaches were tested for the evaluation of the best derivatisation method of trenbolone before GC-MS analysis.…”

Section: Discussionmentioning

confidence: 99%

Screening and confirmatory GC-MS methods for the detection of trenbolone in bovine urine

Woźniak

Matraszek-Żuchowska

Semeniuk

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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Sensitive and selective methods for the screening (GC-MS) and confirmatory analysis (GC-MS/MS) of 17α-and 17β-trenbolone in bovine urine were developed. In the first stage of the analysis, the enzymatic hydrolysis of trenbolone metabolites with glucuronidase AS-HP in acetate buffer (pH 5.2) solution was carried out. Free compounds were extracted from urine with diethyl ether. For the purification of the extract solid phase, extraction with C18 and NH2 columns was applied.

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“…MeOH is the most widely used solvent for extraction of steroids from tissue samples. Impens et al described a procedure for isolating 26 EGAs from muscle and kidney fat with GC-MS/MS detection [192]. Samples were extracted with a mixture of NaAc :…”

Section: Synthetic Steroids and Resorcyclic Acid Lactonesmentioning

confidence: 99%

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Kinsella

O’Mahony

Malone

et al. 2009

Journal of Chromatography A

180

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A comprehensive review is presented on the current trends in sample preparation for isolation of veterinary drugs and growth promotors from foods. The objective of the review is to firstly give an overview of the sample preparation techniques that are applied in field. The review will focus on new techniques and technologies, which improve efficiency and coverage of residues. The underlying theme to the paper is the developments that have been made in multi-residue methods and particularly multi-class methods for residues of licensed animal health products, which have been developed in the last couple of years. The role of multi-class methods is discussed and how they can be accommodated in future residue surveillance.

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Analysis on residues of estrogens, gestagens and androgens in kidney fat and meat with gas chromatography–tandem mass spectrometry

Cited by 71 publications

References 13 publications

A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) method and validation for progestogen multi-residues in feed

A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) method and validation for progestogen multi-residues in feed

Screening and confirmatory GC-MS methods for the detection of trenbolone in bovine urine

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

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