Analysis of urine for 11-nor-.DELTA.9-tetrahydrocannabinol-9-carboxylic acid using Sep-Pak cartridges for sample cleanup

A simple method for plasma cannabinoid separation and quantification

“…The initial solid phase extraction, very selective for cannabinoids (Nakamura et al, 1985), although frequently used for urine samples, had not been applied previously to plasma. The second is a very selective extraction for dansyl derivatives and the final extract is almost free from interfering compounds.…”

Preparation and certification of standard reference material 1507: 11-nor-.DELTA.9-tetrahydrocannabinol-9-carboxylic acid in freeze-dried urine

“…Two aliquots (10 mL each) were removed from each bottle for analysis. Each 10-mL aliquot was placed in a 50-mL centrifuge tube and processed to isolate the THC-9-COOH from urine by using a modification of a published procedure (1). To each 10-mL urine sample, 200 ng of the isotopically labeled internal standard THC-d3-9-COOH in 100 µL of ethanol was added immediately after the sample was removed from the bottle.…”

“…Aluminum is such an element. Despite its being the third most abundant element (1) in the earth's crust (8.2% by weight), dissolved aluminium concentrations in seawater are reported to fall between 0.2 (2) and 120 nM (3) in the trace element range. These low concentrations are a result of the low solubility of aluminosilicate phases during continental weathering (4) and the short residence time of the dissolved form in the ocean.…”

“…Urine samples collected for testing purposes are currently screened by immunoassays, and positive responses are confirmed by a more specific and legally admissible technique such as gas chromatography/ mass spectrometry (GC/MS). Analytical methods for testing for drugs of abuse, such as marijuana, have appeared in the literature in recent years (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). Independent of the method used, reliable control samples such as Standard Reference Materials (SRM's) can be useful tools for improving the quality and ensuring the accuracy of measurements in analytical laboratories.…”

Forensic science

“…A comparison of five methods for the analysis of the major metabolites of A9-THC in urine has been reported (74) and a comparison of six cannabinoid metabolite assays has also been published (75). Solid-supported reagents have also been used in the determination of cannabinoids in plasma (76) and urine (77). Detection of A9-THC in saliva using methods employing RIA (78) and GC/ECD (79) has also been reported.…”