Analysis of urinary nucleosides as potential cancer markers determined using LC–MS technique

Struck-Lewicka, Wiktoria; Kaliszan, Roman; Markuszewski, Michał J.

doi:10.1016/j.jpba.2014.04.022

Cited by 31 publications

(21 citation statements)

References 71 publications

(132 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rate of oxidative DNA damage, RNA turnover and methyltransferase activity increases more in cancer cells than in normal cells. Thus, the urinary content of the nucleoside metabolomes of cancer cells also increases (Struck‐Lewicka et al, ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development of a gas chromatography–mass spectrometry method for breast cancer diagnosis based on nucleoside metabolomes 1‐methyl adenosine, 1‐methylguanosine and 8‐hydroxy‐2′‐deoxyguanosine

Omran

Rashed²,

Darwish

et al. 2019

Biomedical Chromatography

View full text Add to dashboard Cite

Metabolomes are small molecule metabolites (<1000 Da) produced by cellular processes. Metabolomes are close counterparts to the genome, transcriptome and proteome. The aim of this study was to develop a method to detect and quantify candidate nucleoside metabolomes 1‐methyl adenosine (1‐MA), 1‐methylguanosine (1‐MG) and 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) in the urine of patients with breast cancer using gas chromatography–mass spectrometry (GC–MS). The method was applied to urine specimens from patients with breast cancer (n = 56) and benign breast tumors (n = 22), as well as from healthy females (n = 20). The relative standard deviations of precision and repeatability analysis were <10%, and recoveries ranged from 88.5 to 105.6%. Limits of detection were 0.014, 0.012, and 0.018 mg/L for 1‐MA, 1‐MG and 8‐OHdG, respectively. The lower limits of quantitation were 0.056, 0.048 and 0.072 mg/L, respectively. There were significant differences in concentrations of candidate metabolomes between patients with cancer and the healthy individuals, especially for those in the early stages of the disease (p < 0.001). No significant differences were observed between the benign and healthy groups. In conclusion, a reliable GC–MS method for the detection and quantification of 1‐MA, 1‐MG, and 8‐OHdG metabolomes in urine has been developed.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The high concentrations of 1‐MA and 1‐MG in breast cancer samples may be due to RNA turnover and increased methyltransferase activity, both caused by cancer. The higher concentration of 8‐OHdG may be due to oxidative DNA damage, which also occurs in cancer (Struck‐Lewicka et al, ).…”

Section: Discussionmentioning

confidence: 99%

Development of a gas chromatography–mass spectrometry method for breast cancer diagnosis based on nucleoside metabolomes 1‐methyl adenosine, 1‐methylguanosine and 8‐hydroxy‐2′‐deoxyguanosine

Omran

Rashed²,

Darwish

et al. 2019

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…AMPK is a key regulator of metabolism and can negatively regulate tumor proliferation, which functions as a cellular energy sensor primarily regulated by AMP [42]. Another nucleotide, pseudouridine, was reported to be a putative marker in various types of cancer and heart failure [43]. Additionally, several cofactors and vitamins including nicotinamide riboside, NaMN, and nicotinate ribonucleoside were accumulated in MB-treated cells.…”

Section: Discussionmentioning

confidence: 99%

Macranthoidin B Modulates Key Metabolic Pathways to Enhance ROS Generation and Induce Cytotoxicity and Apoptosis in Colorectal Cancer

Fan

Rao

Zhang³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Induction of oxidative stress and reactive oxygen species (ROS) mediated-apoptosis have been utilized as effective strategies in anticancer therapy. Macranthoidin B (MB) is a potent inducer of ROS-mediated apoptosis in cancer, but its mechanism of action is poorly understood. Method: Superoxide production with MB exposure in colorectal cancer (CRC) cells was measured using lucigenin chemiluminescence and real-time PCR. MB’s inhibitory effect on proliferation and viability of CRC cells was determined by proliferation assays. MB’s effect on apoptosis of CRC cells was determined by Western blotting and annexin V-FITC/PI staining. MB’s effect on the growth of CRC xenografts in mice was assessed. An established metabolomics profiling platform combining ultra-performance liquid chromatography-tandem mass spectrometry (LC-MS) with gas chromatography-mass spectrometry (GC-MS) was performed to determine MB’s effect on total metabolite variation in CRC cells. Results: We found that MB increases ROS generation via modulating key metabolic pathways. Using metabolomics profiling platform combining LC-MS with GC-MS, a total of 236 metabolites were identified in HCT-116 cells in which 31 metabolites were determined to be significantly regulated (p ≤ 0.05) after MB exposure. A number of key metabolites revealed by metabolomics analysis include glucose, fructose, citrate, arginine, phenylalanine, and S-adenosylhomocysteine (SAH), suggesting specific modulation of metabolism on carbohydrates, amino acids and peptides, lipids, nucleotide, cofactors and vitamins in HCT-116 CRC cells with MB treatment highly associated with apoptosis triggered by enhanced ROS and activated caspase-3. Conclusion: Our results demonstrate that MB represses CRC cell proliferation by inducing ROS-mediated apoptosis.

show abstract

“…Numerous studies indicate that urinary nucleosides are good candidates as potential cancer biomarkers, especially as compared with carcinoembrionic antigen, carbohydrate antigen 199, carbohydrate antigen 125 or α-fetoprotein, which were reported to have insufficient sensitivity to detect cancer [56,57]. Moreover, determination of numerous nucleoside pattern would be highly desirable in the formation of diseasespecific profile [47].…”

Section: A (μM/mm) N2mg (μM/mm) Case (%) Control (%) or (95% Ci) P-vamentioning

confidence: 99%

Statistical-Based Approach in Potential Diagnostic Application of Urinary Nucleosides in Urogenital Tract Cancer

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

Analysis of urinary nucleosides as potential cancer markers determined using LC–MS technique

Cited by 31 publications

References 71 publications

Development of a gas chromatography–mass spectrometry method for breast cancer diagnosis based on nucleoside metabolomes 1‐methyl adenosine, 1‐methylguanosine and 8‐hydroxy‐2′‐deoxyguanosine

Development of a gas chromatography–mass spectrometry method for breast cancer diagnosis based on nucleoside metabolomes 1‐methyl adenosine, 1‐methylguanosine and 8‐hydroxy‐2′‐deoxyguanosine

Macranthoidin B Modulates Key Metabolic Pathways to Enhance ROS Generation and Induce Cytotoxicity and Apoptosis in Colorectal Cancer

Statistical-Based Approach in Potential Diagnostic Application of Urinary Nucleosides in Urogenital Tract Cancer

Contact Info

Product

Resources

About