Analysis of underivatized oleic acid and related impurities by capillary gas chromatography

Brooks, Stephen H.; Spreen, Russell C.; Zuk, Phyllis E.; Osborn, James A.

doi:10.1002/jhrc.1240130417

Cited by 2 publications

(1 citation statement)

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“…This method facilitated the assay of saturated fatty acids in medical laboratories. Brooks et al [20] described a method for direct quantitation of oleic acid and related fatty acids in pharmaceutical aerosol products. In this method, a GC–FID equipped with DB-FFAP (acidified polyethylene glycol) fused silica column was used for obtaining an impurity profile of oleic acid.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a GC–FID method for quantitative analysis of oleic acid and related fatty acids

Zhang

Wang

Liu

2015

Journal of Pharmaceutical Analysis

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Oleic acid is a common pharmaceutical excipient that has been widely used in various dosage forms. Gas chromatography (GC) has often been used as the quantitation method for fatty acids normally requiring a derivatization step. The aim of this study was to develop a simple, robust, and derivatization-free GC method that is suitable for routine analysis of all the major components in oleic acid USP-NF (United States Pharmacopeia-National Formulary) material. A gas chromatography–flame ionization detection (GC–FID) method was developed for direct quantitative analysis of oleic acid and related fatty acids in oleic acid USP-NF material. Fifteen fatty acids were separated using a DB-FFAP (nitroterephthalic acid modified polyethylene glycol) capillary GC column (30 m×0.32 mm i.d.) with a total run time of 20 min. The method was validated in terms of specificity, linearity, precision, accuracy, sensitivity, and robustness. The method can be routinely used for the purpose of oleic acid USP-NF material analysis.

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Section: Introductionmentioning

confidence: 99%