Analysis of two-stage continuous operation of Escherichia coli containing bacteriophage λ vector

Park, Seong Hoon; Park, Tai Hyun

doi:10.1007/s004499900194

Cited by 7 publications

(5 citation statements)

References 8 publications

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“…In attempting to avoid the disadvantages of the batch process, different strategies have been developed for continuous production of phages. Studies have been conducted in chemostats [ 32 - 35 ] and in two-stage continuous processes in which the host is grown in a first stage and fed to a second stage where the phage is being produced [ 32 , 34 , 36 , 37 ]. These processes allow high volumetric throughputs with smaller fermenter footprints.…”

Section: Introductionmentioning

confidence: 99%

“…One of the main issues is the difficulty in maintaining a stable, consistent, continuous system for the production of phages. Such a situation is only possible if a fine balance among the rate of phage production, the flow rate (and dilution rate) and the rate of host proliferation is maintained [ 32 , 34 ]. These systems are also highly dependent on the threshold population density for the maintenance of the infection [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

“…This is often observed in chemostats - in fact, chemostats are often used to study the co-evolution of host/phage systems [ 39 - 45 ]. The two-stage continuous systems limit to a certain extent, but do not eliminate, co-evolution [ 32 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

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Two-stage, self-cycling process for the production of bacteriophages

Sauvageau

Cooper

2010

Microb Cell Fact

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BackgroundA two-stage, self-cycling process for the production of bacteriophages was developed. The first stage, containing only the uninfected host bacterium, was operated under self-cycling fermentation (SCF) conditions. This automated method, using the derivative of the carbon dioxide evolution rate (CER) as the control parameter, led to the synchronization of the host bacterium. The second stage, containing both the host and the phage, was operated using self-cycling infection (SCI) with CER and CER-derived data as the control parameters. When each infection cycle was terminated, phages were harvested and a new infection cycle was initiated by adding host cells from the SCF (first stage). This was augmented with fresh medium and the small amount of phages left from the previous cycle initiated the next infection cycle. Both stages were operated independently, except for this short period of time when the SCF harvest was added to the SCI to initiate the next cycle.ResultsIt was demonstrated that this mode of operation resulted in stable infection cycles if the growth of the host cells in the SCF was synchronized. The final phage titers obtained were reproducible among cycles and were as good as those obtained in batch productions performed under the same conditions (medium, temperature, initial multiplicity of infection, etc.). Moreover, phages obtained in different cycles showed no important difference in infectivity. Finally, it was shown that cell synchronization of the host cells in the first stage (SCF) not only maintained the volumetric productivity (phages per volume) but also led to higher specific productivity (phage per cell per hour) in the second stage (SCI).ConclusionsProduction of bacteriophage T4 in the semi-continuous, automated SCF/SCI system was efficient and reproducible from cycle to cycle. Synchronization of the host in the first stage prior to infection led to improvements in the specific productivity of phages in the second stage while maintaining the volumetric productivity. These results demonstrate the significant potential of this approach for both upstream and downstream process optimization.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Two-stage, self-cycling process for the production of bacteriophages

Sauvageau

Cooper

2010

Microb Cell Fact

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“…Krysiak-Baltyn et al (2018) developed a computational model to simulate the dynamics of phage population growth and production in a two-stage, self-cycling process. The kinetics of the two-stage continuous production of bacteriophage was modelled by Park and Park (2000).…”

Section: Batch Systemmentioning

confidence: 99%

Chemical and Process Engineering

2022

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The practical applications of bacteriophages are associated with the problems related to the intensification, optimization of process production of this biomaterial and the search for new methods of production. The production of bacteriophages requires a fine balance between the dynamic growth of the bacteriophage and the host. The electromagnetic field (EMF) is a promising biotechnological method for the process production of bacteriophages. This study evaluates the use of various types of EMF to enhance the process. It was found that the process production of bacteriophages is divided into two stages. In the first stage, the influence of various types of EMF on the proliferation process of bacteria (host) was analyzed. Secondly, the process production of bacteriophage was implemented for the optimal infection conditions under the action of the various types of EMF. Moreover, the study demonstrated that the most effective bacteriophage production was the process with the application of the rotating magnetic field (RMF), pulsed magnetic field (PMF) and the static magnetic field (SMF) with negative polarity.

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“…Attempts have been made to overcome the disadvantages of the batch process with continuous phage production. Studies involving chemostats [ 25 , 27 ] have been conducted, as well as two-stage continuous processes or cellstat [ 25 , 28 , 29 , 30 ], which consists of culturing bacteria, separately, in the first stage to feed to a second stage when phages are produced. Although chemostat allows the cultivation of microorganisms at a physiological steady state [ 31 ], the bacterial culture may be less genetically stable, as mutations can occur [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Production of Bacteriophages by Listeria Cells Entrapped in Organic Polymers

Roy

Philippe

Loessner

et al. 2018

Viruses

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Applications for bacteriophages as antimicrobial agents are increasing. The industrial use of these bacterial viruses requires the production of large amounts of suitable strictly lytic phages, particularly for food and agricultural applications. This work describes a new approach for phage production. Phages H387 (Siphoviridae) and A511 (Myoviridae) were propagated separately using Listeria ivanovii host cells immobilised in alginate beads. The same batch of alginate beads could be used for four successive and efficient phage productions. This technique enables the production of large volumes of high-titer phage lysates in continuous or semi-continuous (fed-batch) cultures.

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Analysis of two-stage continuous operation of Escherichia coli containing bacteriophage λ vector

Cited by 7 publications

References 8 publications

Two-stage, self-cycling process for the production of bacteriophages

Two-stage, self-cycling process for the production of bacteriophages

Chemical and Process Engineering

Production of Bacteriophages by Listeria Cells Entrapped in Organic Polymers

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