Analysis of transcripts of heat shock protein genes in silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Chandrakanth, Nalavadi; Ponnuvel, Kangayam M.; Moorthy, S. M.; Sasibhushan, Sirigineedi; Sivaprasad, V.

doi:10.14411/eje.2015.094

Cited by 13 publications

(13 citation statements)

References 55 publications

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“…The fat body and Malpighian tubules were the most sensitive tissues to heat stress (Fig. 6b), which is also the case in silkworm (Chandrakanth et al 2015). The fat body is involved in many metabolic roles.…”

Section: Discussionmentioning

confidence: 66%

Identification and expression analysis of multiple small heat shock protein genes in spruce budworm, Choristoneura fumiferana (L.)

Quan

Duan

Ladd

et al. 2018

Cell Stress and Chaperones

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Fifteen small heat shock protein (sHSP) genes were identified from spruce budworm, Choristoneura fumiferana (L.), an important native forest pest in North America. The transcript levels of each CfHSP were measured under non-stress conditions in all life stages from egg to adult and in five different larval tissues. CfHSP transcript levels showed variation during development, with highest levels in adults and lowest in eggs. Most CfHSP transcripts are highly expressed in larval fat body and Malpighian tubules; two CfHSPs display extremely high expression in the head and epidermis. Upon heat stress, nine CfHSP genes are significantly upregulated, increasing by 50- to 2500-fold depending on developmental stage and tissue type. Upon starvation, eight CfHSPs are upregulated or downregulated, whereas six others retain constant expression. These results suggest that CfHSPs have important and multiple roles in spruce budworm development and in response to heat stress and starvation.

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Section: Discussionmentioning

confidence: 66%

Identification and expression analysis of multiple small heat shock protein genes in spruce budworm, Choristoneura fumiferana (L.)

Quan

Duan

Ladd

et al. 2018

Cell Stress and Chaperones

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“…The results showed that when THTT silkworms were reared at normal temperature for 48 h, the gene expression levels in the midgut were decreased compared to the corresponding controls, except for Bmtal , Bmspz and BmPGRP-LE genes, indicating that the gene expression levels varied in response to high temperature 39, 40 . Bmspz and BmPGRP-LE / LB are related to the Toll and IMD signaling pathways, respectively 28 .…”

Section: Discussionmentioning

confidence: 96%

“…To investigate the effect and mechanism of high temperature treatment on intestinal bacterial population, the expression level changes of genes related to the immune pathway, apoptosis and cell cycle were determined by qRT-PCR after THTT. The results showed that when THTT silkworms were reared at normal temperature for 48 h, the gene expression levels in the midgut were decreased compared to the corresponding controls, except for Bmtal , Bmspz and BmPGRP-LE genes, indicating that the gene expression levels varied in response to high temperature 39 , 40 . Bmspz and BmPGRP-LE / LB are related to the Toll and IMD signaling pathways, respectively 28 .…”

Section: Discussionmentioning

confidence: 96%

Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori

Sun

Kumar

Cao

et al. 2017

Sci Rep

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The silkworm Bombyx mori is a poikilotherm and is therefore sensitive to various climatic conditions. The influence of temperature on the intestinal flora and the relationship between the intestinal flora and gene expression in the silkworm remain unknown. In the present study, changes of the intestinal flora at 48, 96 and 144 h following transient high temperature treatment (THTT) of 37 °C for 8 h were investigated. According to principal component analysis, the abundances of Enterococcus and Staphylococcus showed a negative correlation with other dominant genera. After THTT, the gene expression levels of spatzle-1 and dicer-2 were increased and decreased, respectively, which suggested that the Toll and RNAi pathways were activated and suppressed, respectively. The species-gene expression matrix confirmed that the spatzle-1 and dicer-2 gene expression levels were negatively and positively correlated, respectively, with the abundance of Enterococcus and Staphylococcus in the control. The abundance of Variovorax post-THTT was positively correlated with the spatzle-1 gene expression level, whereas the community richness of Enterococcus was negatively correlated with the spatzle-1 gene expression level and positively correlated with the dicer-2. The results of the present investigation provide new evidence for understanding the relationships among THTT, intestinal flora and host gene expression.

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“…Comparative data from other taxa have shown an association between environmental temperature and either higher constitutive expression of Hsp genes (Bedulina et al, 2010;Li et al, 2014;Mizrahi et al, 2016;Shatilina et al, 2011) or a stronger induced response (Chandrakanth et al, 2015;Dong and Williams, 2011;Haguenauer et al, 2013;Li et al, 2014;Schoville et al, 2012;Shatilina et al, 2011;Tedeschi et al, 2016;Yu et al, 2012). The comprehensive sampling in this study of populations experiencing a wide range of Tmax values should have provided ample power to detect a cline in gene expression if it were present, but Tmax was not related to either constitutive or induced expression in any of the three Hsp genes (Fig.…”

Section: Discussionmentioning

confidence: 99%

Modulation of the heat shock response is associated with acclimation to novel temperatures but not adaptation to climatic variation in the ants Aphaenogaster picea and A. rudis

Cahan

Nguyen

Stanton-Geddes

et al. 2017

Comparative Biochemistry and Physiology Part A: Molecular &

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Ecological diversification into thermally divergent habitats can push species toward their physiological limits, requiring them to accommodate temperature extremes through plastic or evolutionary changes that increase persistence under the local thermal regime. One way to withstand thermal stress is to increase production of heat shock proteins, either by maintaining higher baseline abundance within cells or by increasing the magnitude of induction in response to heat stress. We evaluated whether environmental variation was associated with expression of three heat shock protein genes in two closely-related species of woodland ant, Aphaenogaster picea and A. rudis. We compared adult workers from colonies collected from 25 sites across their geographic ranges. Colonies were maintained at two different laboratory temperatures, and tested for the independent effects of environment, phylogeny, and acclimation temperature on baseline and heat-induced gene expression. The annual maximum temperature at each collection site (Tmax) was not a significant predictor of either baseline expression or magnitude of induction of any of the heat shock protein genes tested. A phylogenetic effect was detected only for basal expression of Hsp40, which was lower in the most southern populations of A. rudis and higher in a mid-range population of possible hybrid ancestry. In contrast, a higher acclimation temperature significantly increased baseline expression of Hsc70-4, and increased induction of Hsp40 and Hsp83. Thus, physiological acclimation to temperature variation appears to involve modulation of the heat shock response, whereas other mechanisms are likely to be responsible for evolutionary shifts in thermal performance associated with large-scale climate gradients.

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Analysis of transcripts of heat shock protein genes in silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Cited by 13 publications

References 55 publications

Identification and expression analysis of multiple small heat shock protein genes in spruce budworm, Choristoneura fumiferana (L.)

Identification and expression analysis of multiple small heat shock protein genes in spruce budworm, Choristoneura fumiferana (L.)

Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori

Modulation of the heat shock response is associated with acclimation to novel temperatures but not adaptation to climatic variation in the ants Aphaenogaster picea and A. rudis

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