Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori

Sun, Zhenli; Kumar, Dhiraj; Cao, Guangwen; Zhu, Liyuan; Liu, Bo; Zhu, Min; Liang, Zi; Kuang, Sulan; Chen, Fei; Feng, Yongjie; Hu, Xiaolong; Xue, Renyu; Gong, Chengliang

doi:10.1038/s41598-017-03565-4

Cited by 26 publications

(18 citation statements)

References 47 publications

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“…Furthermore, we investigated the specific taxa in duodenal, jejunal, and ileal microbiota that were significantly associated with high temperature, which allowed us to determine whether high ambient temperature could change the intestinal microbial community and functions. The present study indicated that abundance of Enterococcus decreased after the ducks were exposed to high temperature, suggesting that the abundance of Enterococcus in the gut contents was significantly affected and sensitive to high temperature, which is consistent with previous results in silkworms ( Sun et al., 2017 ). Enterococcus is characterized by its prebiotic properties in the intestine, which has been reported to have beneficial effects on enhancing the intestinal barrier function ( Pedicord et al., 2016 , Huang et al., 2019 ).…”

Section: Discussionsupporting

confidence: 93%

High-temperature exposure alters the community structure and functional features of the intestinal microbiota in Shaoxing ducks (Anas platyrhynchos)

Tian

Chen

et al. 2020

Poultry Science

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The gut microbiome is a complex ecosystem that contributes to host nutrition and health. However, our current knowledge of the relationship between ambient temperature and gut microbiota of poultry is still limited. The objective of the present study was to characterize the intestinal microbiota of ducks exposed to high ambient temperature. Sixty 60-day-old Shaoxing ducks were allocated to control and heat-treated groups. The ducks in the control group were kept at 25°C, and the ducks in the heat treatment group were raised at 30–40°C, which simulated the temperature change of day and night in summer. After 15 D, the intestinal contents of the duodenum, jejunum, and ileum were obtained from 6 ducks of each group. Genomic DNA was extracted and amplified based on the V4–V5 hypervariable region of 16S rRNA. The results showed that Firmicutes was the dominant bacterial phylum with the highest abundance in the contents of the small intestine of ducks, and the relative abundance of the phylum Firmicutes in all 3 intestinal segments was increased by high temperature. At the genus level, Lactobacillus was found to be the most dominant bacterial genus across 3 gut segments, and its abundance was increased in ducks under heat treatment. Compared with the corresponding intestine segment of control ducks, a total of 36 genera in the duodenum, 19 genera in the jejunum, and 6 genera in the ileum of heat-treated ducks were found to be significantly different in the abundance (linear discriminant analysis score >3.0, P < 0.05). Functional prediction of gut microbiota revealed that high temperature caused changes in the abundance of metabolism and transcription-related pathways. It is noteworthy that most of the altered pathways are related to metabolism. In conclusion, high temperature induced remarkable taxonomic changes in the gut microbiome of ducks, which might be related to the negative effects of high temperature in ducks. Our present study provided an important theoretical ground for high-temperature intervention.

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Section: Discussionsupporting

confidence: 93%

High-temperature exposure alters the community structure and functional features of the intestinal microbiota in Shaoxing ducks (Anas platyrhynchos)

Tian

Chen

et al. 2020

Poultry Science

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Section: Discussionmentioning

confidence: 56%

“…Dominant gut bacteria found in this study, such as Enterobacter, Acinetobacter, Methylobacterium, Wolbachia and Streptococcus, are common in other lepidopteran insects. [64][65][66] There were, however, also potentially pathogenic species, especially associated with A. major and E. similis. Recent advances in studying insect-microbe interactions have shown that entomopathogens play an important role in insect pest biocontrol.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota metabolic potential correlates with body size between mulberry‐feeding lepidopteran pest species

Chen

Xie

Zhang

et al. 2019

Pest Management Science

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BACKGROUND Many insect pests rely on microbial symbionts to obtain nutrients or for defence, thereby allowing them to exploit novel food sources and degrade environmental xenobiotics, including pesticides. Although Lepidoptera is one of the most diverse insect taxa and includes important agricultural pests, lepidopteran microbiotas, particularly functional traits, have not been studied widely. Here, we provide a comprehensive characterization of the gut microbiota across multiple mulberry‐feeding lepidopteran species, resolving both community structure and metabolic potential. RESULTS Our results indicate abundant bacteria inside the gut of larval Lepidoptera. However, even though they were fed the same diet, the structures of the bacterial communities differed in four major mulberry pest species, suggesting host‐specific effects on microbial associations. Community‐level metabolic reconstructions further showed that although taxonomic composition varied greatly, carbohydrate and amino acid metabolism and membrane transporter were key functional capabilities of the gut bacteria in all samples, which may play basic roles in the larval gut. In addition, principal coordinate analysis (PCoA) of gut bacterial‐predicted gene ontologies revealed specialized features of the microbiota associated with these mulberry pests, which were divided into two distinct clusters (macrolepidopterans and microlepidopterans). This pattern became even more prominent when further Lepidoptera species were involved. CONCLUSIONS A suite of gut microbiota metabolic functions significantly correlated with larval size; the metabolism of terpenoids and polyketides, xenobiotics biodegradation and metabolism were specifically enriched in large species, while small larvae had enhanced nucleotide metabolism. Our report paves the way for uncovering the correlation between host phenotype and microbial symbiosis in this notorious insect pest group. © 2019 Society of Chemical Industry

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“…In yeast, increased total CL levels were found under temperature stress (Luévano-Martínez et al, 2015 ). A recent study observed increased total levels of CL and increased linoleic acid (18:2) content in CL, in skeletal muscle in response to overload stimuli (Fajardo et al, 2017 ).…”

Section: Is Essential For Mitochondrial Functionsmentioning

confidence: 99%

Role of Cardiolipin in Mitochondrial Signaling Pathways

Dudek

2017

Front. Cell Dev. Biol.

325

284

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The phospholipid cardiolipin (CL) is an essential constituent of mitochondrial membranes and plays a role in many mitochondrial processes, including respiration and energy conversion. Pathological changes in CL amount or species composition can have deleterious consequences for mitochondrial function and trigger the production of reactive oxygen species. Signaling networks monitor mitochondrial function and trigger an adequate cellular response. Here, we summarize the role of CL in cellular signaling pathways and focus on tissues with high-energy demand, like the heart. CL itself was recently identified as a precursor for the formation of lipid mediators. We highlight the concept of CL as a signaling platform. CL is exposed to the outer mitochondrial membrane upon mitochondrial stress and CL domains serve as a binding site in many cellular signaling events. During mitophagy, CL interacts with essential players of mitophagy like Beclin 1 and recruits the autophagic machinery by its interaction with LC3. Apoptotic signaling pathways require CL as a binding platform to recruit apoptotic factors such as tBid, Bax, caspase-8. CL required for the activation of the inflammasome and plays a role in inflammatory signaling. As changes in CL species composition has been observed in many diseases, the signaling pathways described here may play a general role in pathology.

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Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori

Cited by 26 publications

References 47 publications

High-temperature exposure alters the community structure and functional features of the intestinal microbiota in Shaoxing ducks (Anas platyrhynchos)

High-temperature exposure alters the community structure and functional features of the intestinal microbiota in Shaoxing ducks (Anas platyrhynchos)

Gut microbiota metabolic potential correlates with body size between mulberry‐feeding lepidopteran pest species

Role of Cardiolipin in Mitochondrial Signaling Pathways

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