Analysis of trace metals in single droplet of urine by laser ablation inductively coupled plasma mass spectrometry

A novel calibration strategy based on internal standard–spiked gelatine for quantitative bio-imaging by LA-ICP-MS: application to renal localization and quantification of uranium

“…In this work, in-house solid matrix-matched standards were prepared from uranium-spiked kidney homogenate. Nevertheless, further calibration strategies are found in the literature including the solution-based calibration [27], fabrication xerogel solid calibration standards which are more suitable for the calibration of glasses and silicate matrices [28], the synthesis of metal-spiked polymer films [29], the use of dried droplets or dried matrix spots (DMS) deposited on different substrates [30][31][32][33][34], and the spiking and sectioning of gelatine droplets, whose use has increased noticeably in recent years for the quantitative analysis of biological tissues [35][36][37][38][39][40][41]. For internal standardization, three different approaches are mainly described in the literature for the quantitative elemental imaging of biological samples: (i) the simultaneous nebulization of a standard solution [42], (ii) the use of an element naturally occurring in the sample [43][44][45], and (iii) the addition of an internal standard-spiked layer between the sample and support or on the sample [46][47][48][49][50][51].…”

Atomic spectrometry update. Clinical and biological materials, foods and beverages

“…Given that there is rarely a problem collecting sufficient volume of urine for analysis, the work of Kumtabtim et al 101 may appear somewhat bizarre. Single droplets of urine, dried onto a suitable support, were analysed by LA-ICP-MS.…”

Quantitative elemental analysis of polymers through laser ablation – inductively coupled plasma by using a dried droplet calibration approach, DDCA

“…Therefore, unlike the analytes, carbon is 5 transported to the plasma in both gaseous and particulate phases thus giving rise to a differentiated signal behaviour. 11,22,23 Kumtabtim et al, 24 applied external calibration for urine analysis. Sample droplets were deposited on solid substrates such as paper, glass slide and Teflon sheet.…”