Analysis of trace levels of sulfonamides in fish tissue using micro-scale pipette tip-matrix solid-phase dispersion and fast liquid chromatography tandem mass spectrometry

Shen, Qing; Jin, Renyao; Xue, Jing; Lu, Yanbin; Dai, Zhiyuan

doi:10.1016/j.foodchem.2015.08.050

Cited by 57 publications

(32 citation statements)

References 28 publications

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“…The 96‐well p‐SPE method was validated in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), and intra‐ and inter‐day precision and accuracy, recovery, and stability. The detailed procedure was referred to our previous publication . In brief, a total of six concentration levels ranging from LOQ to 1000 ng/mLwas analyzed for calculating the matrix‐matched standard curves.…”

Section: Methodsmentioning

confidence: 99%

High‐throughput 96‐well solid‐phase extraction for preparation of tetracycline followed by liquid chromatography and mass spectrometry analysis

Kang

Wang

et al. 2018

Electrophoresis

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Tetracyclines abuse has frequently occurred in aquaculture against bacteria, rickettsiae, spirochetes, and mycoplasmas. In this study, a high‐throughput sample preparation method was developed using 96‐well plate solid‐phase extraction (p‐SPE) and the extract was analyzed by ultra‐performance liquid chromatography tandem mass spectrometry (UPLC‐MS/MS). The experimental conditions were optimized such that the pH is 4, the eluting solvent is methanol (2 mL), and the sorbent is hydrophilic‐lipophilic balance (HLB) microsphere. The whole protocol was validated, and it showed that the tetracyclines were linear with correlation coefficients ≥ 0.9990, precision and accuracy (RSD%) in 3.9–6.1%, and mean recoveries of 88.6–103.6%. To exhibit the potential of 96‐well p‐SPE as a routine tool for inspection and quarantine, fresh aquatic samples were tested, and among which positive samples were observed. This method was demonstrated to be promising for the purification and enrichment of tetracyclines with reduced time and labor, and indeed practically and particularly suitable for widespread tetracyclines analysis.

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Section: Methodsmentioning

confidence: 99%

High‐throughput 96‐well solid‐phase extraction for preparation of tetracycline followed by liquid chromatography and mass spectrometry analysis

Kang

Wang

et al. 2018

Electrophoresis

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“…Sulfonamides are a series of amphoteric molecules that have a strong polarity and thermal instability [137,138]; thus, HPLC is typically utilized for the separation of different kinds of sulfonamides with its excellent separation capacity. Detectors coupled with HPLC include UV [71,72], FL [73], DAD [33,74,75], and MS [52,55,139,140]. Among these, the UV detector was most widely used for its inexpensiveness and utility, and the wavelengths of sulfonamides are from 270 to 280 nm or at 255 nm [106].…”

Section: Instrumental Analysismentioning

confidence: 99%

“…As a confirmatory detector, MS can provide information about the molecular structures and quantitative data on the analytes with high sensitivity and selectivity. Furthermore, for the purpose of enhancing sensitivity and accuracy, MS/MS [50,51,53,55] has been utilized for the analyses of sulfonamides. With the advantages and disadvantages mentioned above, MS/MS is outstanding compared with the other detectors.…”

Section: Instrumental Analysismentioning

confidence: 99%

Trends in sensitive detection and rapid removal of sulfonamides: A review

Xie

Huang

Zheng

et al. 2020

J of Separation Science

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Sulfonamides in environmental water, food, and feed are a major concern for both aquatic ecosystems and public health, because they may lead to the health risk of drug resistance. Thus, numerous sensitive detection and rapid removal methodologies have been established. This review summarizes the sample preparation techniques and instrumental methods used for sensitive detection of sulfonamides.Additionally, adsorption and photocatalysis for the rapid removal of sulfonamides are also discussed. This review provides a comprehensive perspective on future sulfonamide analyses that have good performance, and on the basic methods for the rapid removal of sulfonamides. K E Y W O R D Srapid removal, sample preparation techniques, sensitive detection, sulfonamides 1634

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“…Recently, there are several analytical methods based on spectrophotometric or electrochemical detection , most of which for the determination of MG and LMG in a number of aquatic species tissues are based on MS detection, because this detection method provides greater sensitivity, specificity and with better values of the figures of merit. LC‐MS/MS is the most preferred method for determination and confirmation of drug residues . A fast LC–TOF/MS method was employed by Villar‐Pulido et al.…”

Section: Introductionmentioning

confidence: 99%

Development of a PRiME cartridge purification method for rapid determination of malachite green and leucomalachite green in Chinese softshell turtle

et al. 2019

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A high‐throughput PRiME (process, robustness, improvements, matrix effects, ease of use) sample purification procedure was developed to simplify the multiple steps of traditional SPE in extracting the malachite green and leucomalachite green in Chinese softshell turtle (Pelodiscus sinensis). The sample loading volume, extracting solvent type, and pH value of the employed PRiME hydrophilic‐lipophilic balance cartridge for sample purification were optimized to be 3 mL, acetonitrile, and pH 5, respectively. In comparison with traditional SPE, the PRiME process is cost‐effective, solvent‐saving, and simple to operate, which only consists of a passing through step without traditional sorbent conditioning and impurity washing. Afterward, eluate was analyzed by ultra‐performance liquid chromatography‐tandem mass spectrometry, and the proposed method was validated for linearity (R2 > 0.9992), intraday precision (2.44–3.22%), interday precision (3.28–6.58%), sensitivity (LOD ≤ 0.18 μg/kg and, LOQ ≤ 0.60 μg/kg), and recovery (88.7–94.1%, RSD < 6.79%). The results indicated that the PRiME technique can simplify the sample preparation procedure by avoiding the tedious steps, such as conditioning, washing, etc. It would be of significant interest for environmental and food safety applications in the market of Chinese softshell turtle and related products.

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Analysis of trace levels of sulfonamides in fish tissue using micro-scale pipette tip-matrix solid-phase dispersion and fast liquid chromatography tandem mass spectrometry

Cited by 57 publications

References 28 publications

High‐throughput 96‐well solid‐phase extraction for preparation of tetracycline followed by liquid chromatography and mass spectrometry analysis

High‐throughput 96‐well solid‐phase extraction for preparation of tetracycline followed by liquid chromatography and mass spectrometry analysis

Trends in sensitive detection and rapid removal of sulfonamides: A review

Development of a PRiME cartridge purification method for rapid determination of malachite green and leucomalachite green in Chinese softshell turtle

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