Analysis of Thin Hydroxyapatite Layers Formed on Anodic Oxide Titanium after Hydrothermal Treatment in Rat Bone Marrow Cell Culture

Nakasato, Yoshihiro; Takebe, Jun

doi:10.2186/prp.4.32

Cited by 12 publications

(25 citation statements)

References 22 publications

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“…A HA/cpTi surface is compatible with bone tissue,16 and has no adverse effect on immune cells 17. Furthermore, the thin HA layer formed on HA/cpTi increases in stability during the process of bone matrix mineralization 18. These data suggest that the chemical or physical state of the HA/cpTi surface influences the phenotypic expression of cells involved in bone formation.…”

Section: Introductionmentioning

confidence: 90%

“…The morphology of the HA crystals was observed by scanning electron microscopy (SEM) (S‐2300; Hitachi, Tokyo, Japan), which showed that approximately 60% of the surface of the AOFCP was covered with HA crystals 10–12. The precipitated HA crystals were a typical single hexagonal columnar shape and had very high crystallinity 10, 11, 13, 18. This film had a lamellar structure; a thin HA layer of 1 μm was formed on an AOFCP of 4.5 μm, making a total film thickness of 5.5 μm 11.…”

Section: Methodsmentioning

confidence: 99%

“…[10][11][12] The precipitated HA crystals were a typical single hexagonal columnar shape and had very high crystallinity. 10,11,13,18 This film had a lamellar structure; a thin HA layer of 1 mm was formed on an AOFCP of 4.5 mm, making a total film thickness of 5.5 mm. 11 Prior to seeding cultures, the S/cpTi and HA/cpTi disks were exposed to ultraviolet light in a sterile tissue culture hood for 72 h.…”

Section: Sample Preparationmentioning

confidence: 99%

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Anodic oxidation and hydrothermal treatment of commercially pure titanium surfaces increases expression of bone morphogenetic protein‐2 in the adherent macrophage cell line J774A.1

Takebe

Ito

Champagne

et al. 2007

J Biomedical Materials Res

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The surface property of commercially pure titanium (cpTi) was improved by forming a thin hydroxyapatite (HA) layer by anodic oxidation and hydrothermal treatment (HA/cpTi). We hypothesize that the adhesion of macrophages to HA/cpTi surfaces is important to the process of osseointegration. This study investigates the effect of adhesion of macrophages to HA/cpTi surfaces on the expression of bone morphogenetic protein-2 (BMP-2). The murine macrophage cell line J774A.1 was cultured on HA/cpTi and polished cpTi (S/cpTi). Macrophage cell adhesion was examined by SEM, 0-72 h following plating onto HA/cpTi and S/cpTi. BMP-2 gene expression was examined by RT-PCR analysis. The level of BMP-2 secreted into the supernatant was measured using an ELISA assay. The extent of macrophage adhesion increased with time on both the HA/cpTi and S/cpTi surfaces, with a" higher degree of spreading observed on HA/cpTi than onS/cpTi surfaces after 24 or 72 h. The ratio of BMP-2 mRNA was higher on HA/cpTi than on S/cpTi after 24 h (0.348 vs. 0, p < 0.05) and 72 h (0.584 vs. 0.189, p < 0.05). After 24 h, secretion of BMP-2 was detected in cultures grown on HA/cpTi, but not on S/cpTi. After 72 h, secretion of BMP-2 was detected in cultures grown on S/cpTi, but the levels were higher in cultures grown on HA/cpTi. These findings show that macrophages have the capacity to adhere to HA/cpTi endosseous implants and provide a source of osteoinductive cytokines that may play a key role in the process of osseointegration.

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Section: Introductionmentioning

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

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Anodic oxidation and hydrothermal treatment of commercially pure titanium surfaces increases expression of bone morphogenetic protein‐2 in the adherent macrophage cell line J774A.1

Takebe

Ito

Champagne

et al. 2007

J Biomedical Materials Res

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“…One important aspect of the HA coating is its thickness. Thin HA coatings have been found to remain stable during bone matrix mineralization [13], while thicker HA coatings might crack and/or delaminate, with a risk of particulate release [14]. Thin HA coatings have also been shown to promote the same osteogenic response as thicker coatings [8].…”

Section: Introductionmentioning

confidence: 99%

Inflammatory cell response to ultra-thin amorphous and crystalline hydroxyapatite surfaces

Rydén

Omar

Johansson

et al. 2016

J Mater Sci: Mater Med

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It has been suggested that surface modification with a thin hydroxyapatite (HA) coating enhances the osseointegration of titanium implants. However, there is insufficient information about the biological processes involved in the HA-induced response. This study aimed to investigate the inflammatory cell response to titanium implants with either amorphous or crystalline thin HA. Human mononuclear cells were cultured on titanium discs with a machined surface or with a thin, 0.1 μm, amorphous or crystalline HA coating. Cells were cultured for 24 and 96 h, with and without lipopolysaccharide (LPS) stimulation. The surfaces were characterized with respect to chemistry, phase composition, wettability and topography. Biological analyses included the percentage of implant-adherent cells and the secretion of pro-inflammatory cytokine (TNF-α) and growth factors (BMP-2 and TGF-β1). Crystalline HA revealed a smooth surface, whereas the amorphous HA displayed a porous structure, at nano-scale, and a hydrophobic surface. Higher TNF-α secretion and a higher ratio of adherent cells were demonstrated for the amorphous HA compared with the crystalline HA. TGF-β1 secretion was detected in all groups, but without any difference. No BMP-2 secretion was detected in any of the groups. The addition of LPS resulted in a significant increase in TNF-α in all groups, whereas TGF-β1 was not affected. Taken together, the results show that thin HA coatings with similar micro-roughness but a different phase composition, nano-scale roughness and wettability are associated with different monocyte responses. In the absence of strong inflammatory stimuli, crystalline hydroxyapatite elicits a lower inflammatory response compared with amorphous hydroxyapatite.

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“…Over the past sixty years, titanium has been investigated for biomedical applications based on its good biocompatibility (1,2), stability (2)(3)(4), and mechanical properties (2,3). In fact, titanium and titanium alloys osseointegrate with the surrounding bone without forming a fibrous intermediate layer between bone and the implant (5). Fibrous intermediates can delay healing and result in micromotion, ultimately leading to implant failure.…”

Section: Introductionmentioning

confidence: 99%

Advances in calcium phosphate coatings - anodic spark deposition: a review

2014

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High voltage anodization of titanium in the presence of an electrolytic medium containing calcium and phosphate ions has shown improved osteointegration and biocompatibility compared to untreated titanium. Processing parameters influence the unique porous microstructure developed during anodization. These parameters tailor the specific properties of the surface to achieve improved osseointegration of an implant. In addition, subsequent treatment following anodization further alters the microstructure. Numerous studies have examined the influence of these properties on the cellular response and the mechanical properties in terms of coating adhesion and pull-out strength in bone; however, there are conflicting reports on the cellular responses. This review examines those processing parameters and the related influence on cellular responses and mechanical properties. In addition, this review provides a summary of published reports regarding the work related to the advancement of calcium phosphate coatings achieved through high voltage anodization.

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Analysis of Thin Hydroxyapatite Layers Formed on Anodic Oxide Titanium after Hydrothermal Treatment in Rat Bone Marrow Cell Culture

Cited by 12 publications

References 22 publications

Anodic oxidation and hydrothermal treatment of commercially pure titanium surfaces increases expression of bone morphogenetic protein‐2 in the adherent macrophage cell line J774A.1

Anodic oxidation and hydrothermal treatment of commercially pure titanium surfaces increases expression of bone morphogenetic protein‐2 in the adherent macrophage cell line J774A.1

Inflammatory cell response to ultra-thin amorphous and crystalline hydroxyapatite surfaces

Advances in calcium phosphate coatings - anodic spark deposition: a review

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