Analysis of the α-amylase gene sequence and the enzyme activity of Indian rock oyster Saccostrea forskali

Thongsaiklaing, Thanaset; Sehawong, Wimonsiri; Kubera, Anchanee; Ngernsiri, Lertluk

doi:10.1007/s12562-014-0708-z

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…The partial fragment 2 (M. amazonicum alpha-amylase CGI3420101) (Figure 5B) included four cysteine residues typical of the C-terminal region of the alpha-amylase gene found in other species. A glycoside digestive enzyme has already been described in M. amazonicum (Santos et al, 2014) and in other decapod crustaceans: L. vannamei (Van Wormhoudt & Sellos, 1996;Wei, Zhang, Yu, Li, & Xiang, 2014) and Saccostrea forskali (Thongsaiklaing et al, 2014). This enzyme is involved in the hydrolysis of the α-1.4 glycosidic bonds in starch and related compounds (Van der Maarel, Van der Veen, Uitdehaag, Leemhuis, & Dijkhuizen, 2002).…”

Section: Resultsmentioning

confidence: 99%

Prospection of putative genes for digestive enzymes based on functional genome of the hepatopancreas of Amazon river prawn

Rocha

Maciel

Valenti

et al. 2022

Acta Sci. Anim. Sci.

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Over recent years, Macrobrachium amazonicum has become a popular species for shrimp farming due to their fast growth, high survival rates, and marketability. Several studies have focused on the development of new technology for the culture of this species, but many aspects of their nutrition and physiology remain unknown. Thus, the goal of the present study was to obtain transcripts of putative genes encoding digestive enzymes, based on a library of the cDNA from the hepatopancreas of M. amazonicum, sequenced in the Ion TorrentTM platform. We identified fragments of nine genes related to digestive enzymes, acting over proteins, carbohydrates and lipids. Endo and exoproteases were also recorded in the hepatopancreas, indicating adaptation to the digestion of protein-rich foods. Nonetheless, the enzymes involved in the carbohydrate metabolism formed the largest functional group in M. amazonicum, including enzymes related to the digestion of starch, chitin, and cellulose. These findings indicate that the species has a genetic apparatus of a well-adapted omnivorous animal. This information may provide important insights for the selection of ingredients for the formulation of a more appropriate diet to the enzymatic repertoire of M. amazonicum.

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Section: Resultsmentioning

confidence: 99%

Prospection of putative genes for digestive enzymes based on functional genome of the hepatopancreas of Amazon river prawn

Rocha

Maciel

Valenti

et al. 2022

Acta Sci. Anim. Sci.

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“…The enzyme-related protein precursor mRNA is regulated to stimulate transcription and synthesis after ingestion, so that the enzyme precursors are released to promote digestion, absorption, and growth (Yúfera et al, 2018). For example, amylase (AMY) gene is proved to be involved in the growth of razor clam Sinonovacula constricta and Pacific oyster Crassostrea gigas, while cellulase (CEL) can improve the digestibility of food and synthesize glucose to provide energy for the body (Meenu et al, 2014;Thongsaiklaing et al, 2014;Rong et al, 2015;Liu et al, 2017). Moreover, lipase (LIP) gene expression is correlated with feeding status, whereas pepsin (PEP) has important digestive functions in both vertebrates and invertebrates (Liang et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

The Discovery of Circadian Rhythm of Feeding Time on Digestive Enzymes Activity and Their Gene Expression in Sinonovacula constricta Within a Light/Dark Cycle

et al. 2021

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The circadian rhythm has a great impact on the growth, metabolism and development of animals, but little is known about the circadian rhythm of marine bivalves. Understanding of the feeding rhythm is of great significance to increase the yield of razor clam Sinonovacula constricta, an economically important bivalve mollusk. The aim of this experiment was to study the effects of circadian rhythm of feeding time on digestive enzymes activities and their gene expression in S. constricta within a light (ZT8-ZT20)/dark (ZT20-ZT8) cycle. The present results showed that circadian rhythm of feeding rate (FR) was highly associated with digestive enzyme activities and relative expression of their genes. The highest values of FR were basically observed in the night from ZT0-ZT2 and ZT6-ZT8, which were significantly higher than those values in the daytime from ZT12-ZT14 and ZT18-ZT20 (P < 0.05). The digestive enzymes activities displayed the highest values at ZT2 and ZT8, and the lowest at ZT14 and ZT20. Among them, cellulase and pepsin were found to have significantly different activities (P < 0.05), rather than amylase and lipase. Notably, the relative expression of digestive enzyme genes shared the similar pattern with the activities of digestive enzymes. The highest values of relative gene expression of amylase (AMY), lipase (LIP), cellulase (CEL), and pepsin (PEP) were found at ZT2 and ZT8 in the night, while the lowest values were found at ZT14 during the day. It is therefore suggested that the biological clock may regulate the process from feeding to digestion. Furthermore, it might be better to feed at night to reduce cultivating cost and increase economic benefits in the farming industry of S. constricta.

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Genomic structure of the α-amylase gene in the pearl oyster Pinctada fucata and its expression in response to salinity and food concentration

Huang

Guo

et al. 2016

Gene

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Analysis of the α-amylase gene sequence and the enzyme activity of Indian rock oyster Saccostrea forskali

Cited by 3 publications

References 42 publications

Prospection of putative genes for digestive enzymes based on functional genome of the hepatopancreas of Amazon river prawn

Prospection of putative genes for digestive enzymes based on functional genome of the hepatopancreas of Amazon river prawn

The Discovery of Circadian Rhythm of Feeding Time on Digestive Enzymes Activity and Their Gene Expression in Sinonovacula constricta Within a Light/Dark Cycle

Genomic structure of the α-amylase gene in the pearl oyster Pinctada fucata and its expression in response to salinity and food concentration

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