Vitellogenin (Vg) is a precursor of the major yolk protein, an essential nutrient for the embryonic development of oviparous animals including insects. Here, the gene(CceVg [Corcyra cephalonica Vg] ) encoding the Vg (CceVg of moth, C. cephalonica, was cloned and sequenced. The gene sequence was 6,721-bp long and contained 5five introns and six exons that together formed a 5,382-bp open reading frame. The deduced protein (CceVg) consisted of 1,793 amino acid residues, including a 16-amino-acid signal peptide. The putative molecular weight of the primary Vg protein was 202.46 kDa. The CceVg contained all conserved domains and motifs that were commonly found in most insect Vgs except the presence of a polyserine tract at the C-terminal region, which had not been reported in other lepidopteran Vgs. The expression pattern showed that CceVg was first transcribed at a very low level in the early larval stage but disappeared in later stage larva. In female, the CceVg mRNA was detected in early pupal stage and throughout adult stage. Interestingly, the CceVg mRNA was detected only in mated males at low levels, not in the virgin ones. Injection of CceVg double-stranded RNA into early-emergent females caused severely abnormal ovaries.

Tissue engineering aims to utilise biologic mediators to facilitate tissue regeneration. Several recombinant proteins have potential to mediate induction of bone production, however, the high production cost of mammalian cell expression impedes patient access to such treatments. The aim of this study is to produce recombinant human osteopontin (hOPN) in plants for inducing dental bone regeneration. The expression host was Nicotiana benthamiana using a geminiviral vector for transient expression. OPN expression was confirmed by Western blot and ELISA, and OPN was purified using Ni affinity chromatography. Structural analysis indicated that plant-produced hOPN had a structure similar to commercial HEK cell-produced hOPN. Biological function of the plant-produced hOPN was also examined. Human periodontal ligament stem cells were seeded on an OPN-coated surface. The results indicated that cells could grow normally on plant-produced hOPN as compared to commercial HEK cell-produced hOPN determined by MTT assay. Interestingly, increased expression of osteogenic differentiation-related genes, including OSX, DMP1, and Wnt3a, was observed by realtime PCR. These results show the potential of plant-produced OPN to induce osteogenic differentiation of stem cells from periodontal ligament in vitro, and suggest a therapeutic strategy for bone regeneration in the future.

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Pigs, the principal sources of meat for humans, have been crucial to cultures throughout Asia, especially in China and SE Asia, since prehistoric times. Several archaeological studies have used pig remains to elucidate the origin, culture, social evolution, and migration patterns of Asiatic people. However, ancient DNA of these remains in central SE Asia, and in Thailand in particular, has not been investigated to test the historical theories resulting from these archaeological studies. Here, we investigate ancient DNA of pig remains excavated from Pong Takhop archaeological site, central Thailand aged at least 3000 BP. The phylogenetic tree we obtained suggests that ancient Thai pigs were descended from ancient Chinese pigs. The tree topology further suggests that these ancient pigs had multiple origins, which were probably generated by multiple waves of migration of ancient Chinese pigs from 4000-3000 BP. Most of these ancient Thai pigs left their lineages as modern Thai pigs observed in northern Thailand. The contrasting cluster of pure modern Thai pigs suggested that these pigs might be descended from non-Chinese ancestors, possibly the native SE Asian ancestors.