Analysis of the Volatile Components of Five Turkish Rhododendron Species by Headspace Solid-Phase Microextraction and GC-MS (HS-SPME-GC-MS)

Abstract

Volatile constituents of various solvent extracts (n-hexane, CH 2 Cl 2 , H 2 O) of 15 different organs (leaves, flowers, fruits) of five Rhododendron species (Ericaceae) growing in Turkey were trapped with headspace solid-phase microextraction (HS-SPME) technique and analyzed by GC-MS. A total of 200 compounds were detected and identified from organic extracts, while the water extracts contained only traces of few volatiles. The CH 2 Cl 2 extract of the R. luteum flowers was found to exhibit the most diverse composition: 34 compounds were identified, with benzyl alcohol (16.6%), limonene (14.6%) and p-cymene (8.4%) being the major compounds. The CH 2 Cl 2 -solubles of R. x sochadzeae leaves contained only phenyl ethyl alcohol. This study indicated appreciable intra-specific variations in volatile compositions within the genus. Different anatomical parts also showed altered volatile profiles. This is the first application of HS-SPME-GC-MS on the volatiles of Rhododendron species.

Studies on the floral anatomy and scent chemistry of titan arum(Amorphophallus titanum, Araceae)

“…The fiber was pushed through the film layer for exposure to the headspace of the extract for 15 min at 40 °C. The fiber was then inserted immediately into the injection port of the GC-MS for desorption of the adsorbed volatile compounds for analysis (Tasdemir et al, 2003).…”

Volatile composition of Anatolian propolis by headspace-solid-phase microextraction (HS-SPME), antimicrobial activity against food contaminants and antioxidant activity

“…Identification of the volatile components was carried out by comparison of their relative retention times with those of authentic samples or by comparison of their relative retention indices (RRI) to a series of n-alkanes (C9-20). Computer matching against commercial libraries (Adams Library, Wiley GC/MS Library, MassFinder 3 Library) and in-house "Baser Library of Essential Oil Constituents" built up of genuine compounds and components of known oils, as well as MS literature data was used for the identification (Tasdemir et al, 2003;Demirci et al, 2007;Polatoglu et al, 2012).…”

“…The adsorption is performed by immersing the pre-coated fibre in a liquid sample or exposing it to the headspace above a liquid or solid sample. In the last two decades, HS-SPME has found applications in environmental, biological, food and pharmaceutical analyses (Cserháti, 2010;Tasdemir et al, 2003). To the best of our knowledge, there is only one previous study on the volatiles of propolis using HS-SPME (Yildirim et al, 2004).…”

Rhododendrons and Grayanotoxins

“…Analytical methods for the detection of grayanotoxin I include thin -layer chromatography, 10 GS/MS, 29 and liquid chromatography/mass spectrometry. 30 The low vapor pressure and heat instability of grayanotoxins necessitates derivatization prior to analysis when using gas chromatography.…”