Analysis of the Viability and Morphology of Gingival Cells on Materials Used in Novel Prosthetic Components: In Vitro Study

Cecato, Rafael Cury; Martinez, Elizabeth Ferreira; Benfatti, César Augusto Magalhães

doi:10.5005/jp-journals-10024-3271

Cited by 1 publication

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…50% increase compared to the titanium alloy). Cecato et al [73] showed an increased rate of growth of gingival cells (keratinocytes and fibroblasts) on steel compared to titanium or polyether ether ketone (PEEK).…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that laser-induced surface topographies, such as LIPSS, exhibit excellent antibacterial adhesion behavior compared to polished surfaces [74][75][76]. Surface micro/nanostructures reduce the contact area between materials and bacteria [73]. Furthermore, it is assumed that the surface oxidation that occurs during laser irradiation increases the surface energy of the materials, and thus additionally impairs the interactions between the bacterial membranes and the material surfaces [74,75].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Impact of Ultrashort Pulse Laser Structuring of Metals on In-Vitro Cell Adhesion of Keratinocytes

Staehlke,

Barth,

Muench

et al. 2024

JFB

View full text Add to dashboard Cite

Besides the need for biomaterial surface modification to improve cellular attachment, laser-structuring is favorable for designing a new surface topography for external bone fixator pins or implants. The principle of this study was to observe how bioinspired (deer antler) laser-induced nano–microstructures influenced the adhesion and growth of skin cells. The goal was to create pins that allow the skin to attach to the biomaterial surface in a bacteria-proof manner. Therefore, typical fixator metals, steel, and titanium alloy were structured using ultrashort laser pulses, which resulted in periodical nano- and microstructures. Surface characteristics were investigated using a laser scanning microscope and static water contact angle measurements. In vitro studies with human HaCaT keratinocytes focused on cell adhesion, morphology, actin formation, and growth within 7 days. The study showed that surface functionalization influenced cell attachment, spreading, and proliferation. Micro-dimple clusters on polished bulk metals (DC20) will not hinder viability. Still, they will not promote the initial adhesion and spreading of HaCaTs. In contrast, additional nanostructuring with laser-induced periodic surface structures (LIPSS) promotes cell behavior. DC20 + LIPSS induced enhanced cell attachment with well-spread cell morphology. Thus, the bioinspired structures exhibited a benefit in initial cell adhesion. Laser surface functionalization opens up new possibilities for structuring, and is relevant to developing bioactive implants in regenerative medicine.

show abstract

Section: Discussionmentioning

confidence: 99%