Analysis of the Total Biflavonoids Extract from Selaginella doederleinii by HPLC-QTOF-MS and Its In Vitro and In Vivo Anticancer Effects

Abstract

Selaginella doederleinii Hieron has been traditionally used as a folk antitumor herbal medicine in China. In this paper, the phytochemical components of the total biflavonoids extract from S. doederleinii were studied by using high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF MS/MS) in negative ion mode, and their in vitro and in vivo anticancer effects were evaluated. Four types of biflavonoids from S. doederleinii, including IC3′–IIC8′′, IC3′–IIC6′′, IC3′–IIC3′′′, and C–O linked biflavonoids were examined originally using QTOF MS/MS. The fragmentation behavior of IC3′–IIC3′′′ linked biflavonoids was reported for the first time. A total of twenty biflavonoids were identified or tentatively characterized and eight biflavonoids were found from S. doederleinii for the first time. Furthermore, the 3-(4,5-Dimethyl-2-thizolyl)-2,5-diphenyltertazolium bromide (MTT) assay and xenograft model of mouse lewis lung cancer(LLC) in male C57BL/6 mice revealed favorable anticancer properties of the total biflavonoids extracts from S. doederleinii. The results of this work could provide useful knowledge for the identification of biflavonoids in herbal samples and further insights into the chemopreventive function of this plant.

Kandungan Senyawa Kimia Dan Bioaktivitas Dari Selaginella Doederleinii Hieron

“…Dalam review ini, belum ditemukan studi aktivitas antioksidan secara in vivo sehingga perlu dilakukan studi aktivitas antioksidan secara in vivo untuk melengkapi database efek farmakologi dari Selaginella doederleinii Hieron. Sembilan senyawa biflavone (amentoflavone, robustaflavone, 4′-methoxy robustaflavone, podocarpusflavone, hinokiflavone, ginkgetin, dan heveaflavone) menunjukkan aktivitas antioksidan yang baik menggunakan metode DPPH (Yao et al 2017). Ekstrak etil asetat dari Selaginella doederleinii Hieron juga berpotensi sebagai sumber bioaktif alami yang mudah didapat dari alam.…”

Antitumor activity in colorectal cancer induced by hinokiflavone

“…a, molecular formula C30H18O10) with high stability is extracted from Selaginella tamariscina , Juniperus phoenicea , Rhus succedanea , and several other plants and is commonly used in oriental medicines . Hinokiflavone is a biflavonoid active constituent of flavonoids, a class of plant secondary metabolites, which have been reported to have multiple biological properties, including antioxidative, anti‐inflammatory, antimicrobial, and antitumor . Theodoratou et al .…”

“…[11][12][13] Hinokiflavone is a biflavonoid active constituent of flavonoids, a class of plant secondary metabolites, which have been reported to have multiple biological properties, including antioxidative, anti-inflammatory, antimicrobial, and antitumor. 12,[14][15][16][17] Theodoratou et al found strong and linear inverse associations between flavonoids and CRC risk by using data from a national prospective case-control study in Scotland. 18 Kang et al found that ochnaflavone, a natural biflavonoid, could induce cell cycle arrest and apoptosis in colon cancer cells.…”

Antioxidant activity of ethanolic extract of three Selaginella species from Java Island, Indonesia

“…Ethanolic extracts of S. involvens, S. intermedia, S. inaequalifolia, and S. tenera have been reported to possess antioxidants activity (Sivaraman et al 2013). Yao et al (2017) reported that S. doederleinii contains eight biflavonoid types which enhanced the antitumor immune response in the mouse lung cancer model. Syaefudin et al (2016) reported that nanoparticle of S. doederleinii leaves extract could inhibit A549 cancer cell growth.…”