Analysis of the Serum Peptidomics Profile for Cats With Sarcomeric Gene Mutation and Hypertrophic Cardiomyopathy

Abstract

Background: Hypertrophic cardiomyopathy (HCM) has a complex phenotype that is partly explained by genetic variants related to this disease. The serum peptidome profile is a promising approach to define clinically relevant biomarkers. This study aimed to classify peptide patterns in serum samples between cats with sarcomeric gene mutations and normal cats.Materials and Methods: In the total serum samples from 31 cats, several essential proteins were identified by peptidomics analysis. The 5,946 peptides were differentially expressed in cats with sarcomeric gene mutations compared with cats without mutations.Results: Our results demonstrated characteristic protein expression in control cats, Maine Coon cats, and Maine Coon cats with gene mutations. In cats with gene mutations, peptide expression profiling showed an association with three peptides, Cytochrome 3a132 (CYP3A132), forkhead box O1 (FOXO1), and ArfGAP, with GTPase domains, ankyrin repeats, and PH domain 2 (AGAP2).Discussion: The serum peptidome of cats with mutations might provide supporting evidence for the dysregulation of metabolic and structural proteins. Genetic and peptidomics investigations may help elucidate the phenotypic variability of HCM and treatment targets to reduce morbidity and mortality of HCM in cats.

Development of a Loop-Mediated Isothermal Amplification Assay Coupled With a Lateral Flow Dipstick Test for Detection of Myosin Binding Protein C3 A31P Mutation in Maine Coon Cats

“…According to our findings in the recruited study animals, the MYBPC3-A31P mutation rate was 14.55%, which is low. In concordance with our recent prior publication, the mutation rates of MYBPC3-A31P and A74T in Maine Coon cats accounted for 21.43% in Thailand ( 23 ). The prevalence of MYBPC3-A31P mutations worldwide (not included in Thailand) is around 34% ( 27 ).…”

“…The stored blood was aliquoted and kept at −20°C until DNA extraction. As described previously, the process of DNA extraction was conducted via Blood Genomic DNA Extraction Mini Kit (Favorgen, Taiwan) regarding manufacturer's recommendations ( 23 ).…”

Echocardiography and MALDI-TOF Identification of Myosin-Binding Protein C3 A74T Gene Mutations Involved Healthy and Mutated Bengal Cats

“…Advancement proteomic approaches currently become a novel instrument used in clinical diagnosis not only in humans but also in veterinary medicine due to reliable and rapid interpretation. In our earlier publication, the identification of peptides and proteins related to familial HCM was reported in Maine coon cats via matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry [ 19 ]. Analysis of peptides relied on unique peptide mass fingerprints (PMFs), and clusters of individual groups in HCM cats were identified using a three-dimensional principal component analysis (3D-PCA) scatterplot.…”