Analysis of the Role and Regulation Mechanism of hsa-miR-147b in Lung Squamous Cell Carcinoma Based on The Cancer Genome Atlas Database

Ke, Di; Guo, Qiang; Fan, Teng-Yang; Xiao, Xue

doi:10.1089/cbr.2020.4187

Cited by 13 publications

(12 citation statements)

References 26 publications

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“…This result has also been observed in a regulatory network analysis [35]. Among all of the residual effects, LRAT, PLEKHA6, ACOT7, KLK6, PLEKHB1, FGFRL1, and FPR2 are associated with prognosis of LUSC patients from existing studies [36][37][38][39][40][41]. We adopt a random sampling approach and apply log-rank test for assessment in order to evaluate prediction.…”

Section: Lung Squamous Cell Carcinoma (Lusc) Datasupporting

confidence: 53%

Integrating Multi–Omics Data for Gene-Environment Interactions

Fan

et al. 2021

BioTech

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Gene-environment (G×E) interaction is critical for understanding the genetic basis of complex disease beyond genetic and environment main effects. In addition to existing tools for interaction studies, penalized variable selection emerges as a promising alternative for dissecting G×E interactions. Despite the success, variable selection is limited in terms of accounting for multidimensional measurements. Published variable selection methods cannot accommodate structured sparsity in the framework of integrating multiomics data for disease outcomes. In this paper, we have developed a novel variable selection method in order to integrate multi-omics measurements in G×E interaction studies. Extensive studies have already revealed that analyzing omics data across multi-platforms is not only sensible biologically, but also resulting in improved identification and prediction performance. Our integrative model can efficiently pinpoint important regulators of gene expressions through sparse dimensionality reduction, and link the disease outcomes to multiple effects in the integrative G×E studies through accommodating a sparse bi-level structure. The simulation studies show the integrative model leads to better identification of G×E interactions and regulators than alternative methods. In two G×E lung cancer studies with high dimensional multi-omics data, the integrative model leads to an improved prediction and findings with important biological implications.

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Section: Lung Squamous Cell Carcinoma (Lusc) Datasupporting

confidence: 53%

Integrating Multi–Omics Data for Gene-Environment Interactions

Fan

et al. 2021

BioTech

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“…Thus, circRNAs have become a hot topic in carcinoma research. Recently, although more and more reports have suggested that some circRNAs are involved in the progression of multiple cancers and chemoresistance [ 32 , 33 ], the functions of the majority of circRNAs remain unclear. Herein, we investigated the roles and regulatory mechanism of circ_0006168 in Taxol resistance of ESCC.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA circ_0006168 enhances Taxol resistance in esophageal squamous cell carcinoma by regulating miR-194-5p/JMJD1C axis

Wang

et al. 2021

Cancer Cell Int

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Background Chemoresistance is one of the major obstacles for cancer therapy in the clinic. Circular RNAs (circRNAs) are involved in the pathogenesis of esophageal squamous cell carcinoma (ESCC) and chemoresistance. This study aimed to explore the role and molecular mechanism of circ_0006168 in Taxol resistance of ESCC. Methods The expression levels of circ_0006168, microRNA-194-5p (miR-194-5p) and jumonji domain containing 1C (JMJD1C) were measured by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. The half-inhibition concentration (IC50) value of Taxol was evaluated by Cell Counting Kit-8 (CCK-8) assay. Cell proliferation was evaluated by CCK-8 and colony formation assays. Cell migration and invasion were detected by transwell assay. Cell apoptosis was determined by flow cytometry. The interaction between miR-194-5p and circ_0006168 or JMJD1C was predicted by bioinformatics analysis (Circinteractome and TargetScan) and verified by dual-luciferase reporter and RNA Immunoprecipitation (RIP) and RNA pull-down assays. The mice xenograft model was established to investigate the roles of circ_0006168 in vivo. Results Circ_0006168 and JMJD1C were upregulated and miR-194-5p was downregulated in ESCC tissues, ESCC cells, and Taxol-resistant cells. Functionally, knockdown of circ_0006168 or JMJD1C increased Taxol sensitivity of ESCC in vitro via inhibiting cell proliferation, migration and invasion, and promoting apoptosis. Moreover, circ_0006168 could directly bind to miR-194-5p and JMJD1C was verified as a direct target of miR-194-5p. Mechanically, circ_0006168 was a sponge of miR-194-5p to regulate JMJD1C expression in ESCC cells. Furthermore, JMJD1C overexpression reversed the promotive effect of circ_0006168 knockdown on Taxol sensitivity. Besides, circ_0006168 silence suppressed tumor growth in vivo. Conclusion Circ_0006168 facilitated Taxol resistance in ESCC by regulating miR-194-5p/JMJD1C axis, providing a promising therapeutic target for ESCC chemotherapy.

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“…Numerous studies have shown that miRNA can regulate gene expression by playing a key role in carcinogenesis or tumor suppression 36 . For example, miR-296-5p and miR-28-3p showed elevated and decreased expression in the blood of GC patients, respectively, and both high miR-296-5p expression and low miR-28-3p expression predicted poor GC patient survival 37 . To our knowledge, the expression levels of miRNA-30c-5p were decreased in the different types of cancers, and the miR-30c-5p might be as a tumor suppressor gene by inhibiting proliferation, migration, invasion, and metastasis of cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Effects and prognostic values of miR-30c-5p target genes in gastric cancer via a comprehensive analysis using bioinformatics

Liu

Zhang

et al. 2021

Sci Rep

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Gastric cancer (GC) is a common cancer and the leading cause of cancer-related death worldwide. To improve the diagnosis and treatment of GC, it is necessary to identify new biomarkers by investigating the cellular and molecular mechanisms. In this study, miR-30c-5p expression was significantly down-regulated in GC tissues by comprehensive analysis using multiple databases. The target genes of miR-30c-5p with up-regulated expression level in GC were identified, including ADAM12 (a disintegrin and metalloproteinase12), EDNRA (the Endothelin receptor type A), STC1 (stanniocalcin 1), and CPNE8 (the calcium-dependent protein, copine 8). The expression level of ADAM12 was significantly related to depth of invasion (p = 0.036) in GC patients. The expression level of EDNRA was significantly related to grade (P = 0.003), depth of invasion (P = 0.019), and lymphatic metastasis (P = 0.001). The expression level of CPNE8 was significantly related to grade (P = 0.043) and TNM stage (P = 0.027).Gene set enrichment analysis showed that they might participate in GC progression through cancer-related pathways. CIBERSORT algorithm analysis showed that their expressions were related to a variety of tumor-infiltrating immune cells. The higher expression of those target genes might be the independent risk factor for poor survival of GC patients, and they might be potential prognostic markers in GC patients.

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Analysis of the Role and Regulation Mechanism of hsa-miR-147b in Lung Squamous Cell Carcinoma Based on The Cancer Genome Atlas Database

Cited by 13 publications

References 26 publications

Integrating Multi–Omics Data for Gene-Environment Interactions

Integrating Multi–Omics Data for Gene-Environment Interactions

Circular RNA circ_0006168 enhances Taxol resistance in esophageal squamous cell carcinoma by regulating miR-194-5p/JMJD1C axis

Effects and prognostic values of miR-30c-5p target genes in gastric cancer via a comprehensive analysis using bioinformatics

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