Effects and prognostic values of miR-30c-5p target genes in gastric cancer via a comprehensive analysis using bioinformatics

Abstract

Gastric cancer (GC) is a common cancer and the leading cause of cancer-related death worldwide. To improve the diagnosis and treatment of GC, it is necessary to identify new biomarkers by investigating the cellular and molecular mechanisms. In this study, miR-30c-5p expression was significantly down-regulated in GC tissues by comprehensive analysis using multiple databases. The target genes of miR-30c-5p with up-regulated expression level in GC were identified, including ADAM12 (a disintegrin and metalloproteinase12), EDNRA (the Endothelin receptor type A), STC1 (stanniocalcin 1), and CPNE8 (the calcium-dependent protein, copine 8). The expression level of ADAM12 was significantly related to depth of invasion (p = 0.036) in GC patients. The expression level of EDNRA was significantly related to grade (P = 0.003), depth of invasion (P = 0.019), and lymphatic metastasis (P = 0.001). The expression level of CPNE8 was significantly related to grade (P = 0.043) and TNM stage (P = 0.027).Gene set enrichment analysis showed that they might participate in GC progression through cancer-related pathways. CIBERSORT algorithm analysis showed that their expressions were related to a variety of tumor-infiltrating immune cells. The higher expression of those target genes might be the independent risk factor for poor survival of GC patients, and they might be potential prognostic markers in GC patients.

Artificial neural network‐based gene screening and immune cell infiltration analysis of osteosarcoma feature

“…To delve deeper into the level and differences in immune cell infiltration between the OS and NC groups, we utilized R software packages e1071 (David Meyer [aut, cre], et al, https://cran.r-project.org/web/ packages/e1071/) and preprocessCore (Ben Bolstad [aut], et al, https://github.com/bmbolstad/preprocessCore) to analyze the immune cell infiltration in the sample groups, thereby determining their infiltration levels. 45 Differential analysis was also conducted on the immune cell infiltration levels between the two groups. 46 The results were then visualized using a histogram and a violin plot, 47 with a p value less than 0.05 considered statistically significant.…”

Combined scRNA-Seq and bulk RNA-Seq analysis suggests that ITGB2 has potential as a prognostic factor for the immune microenvironment of gastric cancer tumours and as an indicator of tumour microenvironment remodelling.

“…However, the outcomes for advanced patients remain unsatisfactory. Therefore, the identification of new biomarkers and effective therapeutic targets for gastric cancer through the study of cellular and molecular mechanisms is needed [3,4].…”

Investigações sobre os mecanismos moleculares da angiogênese com base em dados de expressão gênica num modelo murino, e sua aplicação no prognóstico do câncer de mama

“…Da mesma família do miR-30e, o miR-30c-5p também é associado ao papel de supressor tumoral. Seu nível de expressão é um fator prognóstico para câncer de estômago, onde a baixa expressão desse miRNA é associada a um prognóstico ruim para a doença (HU et al, 2021). Em células endoteliais do fígado, foi observado que o miR-30c-5p inibe a angiogênese ao se ligar ao gene DLL4, reduzindo os efeitos da fibrose hepática (GU et al, 2021).…”