1988
DOI: 10.1128/jb.170.10.4501-4508.1988
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Analysis of the Pseudomonas solanacearum polygalacturonase encoded by pglA and its involvement in phytopathogenicity

Abstract: A major endopolygalacturonase excreted by Pseudomonas solanacearum was purified to >95 % homogeneity and shown to have an isoelectric point of 9.0 and a subunit molecular mass of 52 kilodaltons (kDa). The gene encoding this enzyme (pglA) was isolated from a genomic library of P. solanacearum DNA based on its expression in Escherichia coli and shown to be contained on a 1.8-kilobase DNA fragment. The identity of the pglA gene product and the 52-kDa polygalacturonase was demonstrated by immunoadsorption and isoe… Show more

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Cited by 87 publications
(73 citation statements)
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References 41 publications
(22 reference statements)
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“…After incubation at 30°C for 24 h, 1 mL of 0.5% soft agar containing 1% hexadecyltrimethylammonium bromide was overlaid on the culture medium to visualize pectate lyase activity. 28) Pectate lyase activity was assessed by measuring the length which pulled the diameter of the colony from that of clear zone formed by pectate lyase activity. Pectate lyase assay was repeated five times.…”
Section: Extraction and Identification Of Ahlsmentioning
confidence: 99%
“…After incubation at 30°C for 24 h, 1 mL of 0.5% soft agar containing 1% hexadecyltrimethylammonium bromide was overlaid on the culture medium to visualize pectate lyase activity. 28) Pectate lyase activity was assessed by measuring the length which pulled the diameter of the colony from that of clear zone formed by pectate lyase activity. Pectate lyase assay was repeated five times.…”
Section: Extraction and Identification Of Ahlsmentioning
confidence: 99%
“…In this study, PG was purified from P. solanacearum AR containing pDR340, a broad host range plasmid construct overexpressing the pglA gene from a lac promoter (Schell et al, 1988). To simplify purification P. solanacearum strain AR was used as host because it harbors a phcA mutation that simultaneously increases pglA expression and blocks expression of many other extracellular proteins and viscous extracellular polysaccharides (Schell et al, 1994;Huang, Denny, & Schell, 1993).…”
Section: Introductionmentioning
confidence: 99%
“…Pseudomonas solanacearum is a soil-borne plant pathogen that causes wilting diseases in many crop plants worldwide (Hayward, 1991). Schell, Roberts & Denny (1988) and Denny, Carney & Schell (1990) were able to demonstrate that a major secreted PG encoded by the pglA gene plays a significant role in disease, but is not absolutely necessary for pathogenesis. P. solanacearum produces at least two other PG's besides PglA, both are apparently exopolygalacturonases (Schell, Denny & Huang, 1994).…”
mentioning
confidence: 99%
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