Analysis of the products from enzymatic scouring of cotton

Abstract

This article discusses the analysis of the hydrolysis products from one-step scouring of cotton using pectinase and two-step scouring of cotton using lipase then cellulase, protease then cellulase, or lipase/protease then cellulase, to improve water absorbency of cotton. UV spectrophotometric analysis indicated that the pectinase scouring process produced approximately 18-fold higher amounts of reducing sugars and galacturonic acid than any of the two-step scouring processes. The production rate of reducing sugars and galacturonic acid from most of the scouring processes showed a decrease with an increase in time. HPLC analysis revealed that the lipase/protease/cellulase scouring processes produced approximately 5-fold higher amounts of 17 amino acids than the pectinase scouring process. GC analysis for 18 fatty acids (C(8)-C(24)) revealed that three major fatty acids, palmitic acid, stearic acid, and behenic acid, were found on both the scoured and the unscoured fabrics. Scoured fabrics were tested for content of proteins, extractable components, waxes, and anionic components including pectins, and some differences among the fabric scoured with different enzyme combinations were found.

Characterization of Thermobifida fusca Cutinase-Carbohydrate-Binding Module Fusion Proteins and Their Potential Application in Bioscouring

“…Traditionally, this process is performed by hot hydrolysis in alkaline medium, which not only consumes large quantities of water and energy but also causes severe pollution and fiber damage (20,21,33). Therefore, environment-friendly scouring methods based on biocatalysts have been actively sought (2,30,36).Cutinase is a multifunctional esterase capable of degrading the cutin component of the cuticle. Earlier reports showed that the fungal cutinase from Fusarium solani pisi has potential use for cotton cuticle degradation and exhibits a good synergistic effect with pectinase, an enzyme utilized to degrade pectin, in the scouring of cotton fiber (1,7,8,14).…”

“…Traditionally, this process is performed by hot hydrolysis in alkaline medium, which not only consumes large quantities of water and energy but also causes severe pollution and fiber damage (20,21,33). Therefore, environment-friendly scouring methods based on biocatalysts have been actively sought (2,30,36).…”

Investigation of the Optimum Pretreatment Conditions for the Knitted Fabric Derived from PLA/Cotton Blend

“…The advantage of enzymatic scouring is that the processing temperature can be reduced because most enzymatic scouring is normally performed at lower temperature and pH compared with alkali scouring. Furthermore, enzymes do not deteriorate cellulose structure, hence, the fiber strength is not affected [8].…”

Properties of cotton fabrics treated by protease and its multienzyme combinations

“…9,10 The implementation of proteases as scouring agents is well documented, but their efficiency is controversial. [11][12][13] The treatment of textiles with a wide range of proteases was not effective in improving their water absorbency. 9,14,15 However, a significant increase in the water retention capacity was reported for fabrics treated with a serine-type protease from Bacillus subtilis, which was suggestive of the effective removal of proteins from the lumen.…”