Analysis of the polyphosphate-accumulating microflora in phosphorus-eliminating, anaerobic-aerobic activated sludge systems by using diaminopropane as a biomarker for rapid estimation of Acinetobacter spp

Auling, Georg; Pilz, Frank; Busse, Hans‐Jürgen; Karrasch, Simone; Streichan, Marlies; Schön, G.

doi:10.1128/aem.57.12.3585-3592.1991

Cited by 78 publications

(25 citation statements)

References 32 publications

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“…1). Physical separation of the gene pair from the rest of the NIS biosynthetic cluster may explain why 1,3-diaminopropane is accumulated to a relatively high level in A. baumannii, a species which seems to synthesize no polyamine other than 1,3-diaminopropane (Auling et al, 1991;Hamana and Matsuzaki, 1992). Transposon insertion mutants of the DABAAT-and DABA DC-encoding genes of A. baumannii no longer exhibit surfaceassociated motility and do not make 1,3-diaminopropane (Skiebe et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Evolution of a novel lysine decarboxylase in siderophore biosynthesis

Burrell

Hanfrey

Kinch

et al. 2012

Molecular Microbiology

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SummaryStructural backbones of iron-scavenging siderophore molecules include polyamines 1,3-diaminopropane and 1,5-diaminopentane (cadaverine). For the cadaverine-based desferroxiamine E siderophore in Streptomyces coelicolor, the corresponding biosynthetic gene cluster contains an ORF encoded by desA that was suspected of producing the cadaverine (decarboxylated lysine) backbone. However, desA encodes an L-2,4-diaminobutyrate decarboxylase (DABA DC) homologue and not any known form of lysine decarboxylase (LDC). The only known function of DABA DC is, together with L-2,4-aminobutyrate aminotransferase (DABA AT), to synthesize 1,3-diaminopropane. We show here that S. coelicolor desA encodes a novel LDC and we hypothesized that DABA DC homologues present in siderophore biosynthetic clusters in the absence of DABA AT ORFs would be novel LDCs. We confirmed this by correctly predicting the LDC activity of a DABA DC homologue from a Yersinia pestis siderophore biosynthetic pathway. The corollary was confirmed for a DABA DC homologue, adjacent to a DABA AT ORF in a siderophore pathway in the cyanobacterium Anabaena variabilis, which was shown to be a bona fide DABA DC. These findings enable prediction of whether a siderophore pathway will utilize 1,3-diaminopropane or cadaverine, and suggest that the majority of bacteria use DABA AT and DABA DC for siderophore, rather than norspermidine/ polyamine biosynthesis.

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Section: Discussionmentioning

confidence: 99%

Evolution of a novel lysine decarboxylase in siderophore biosynthesis

Burrell

Hanfrey

Kinch

et al. 2012

Molecular Microbiology

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“…Species identification commonly involved subjecting plate colonies to numerous physiological and biochemical tests or using Analytical Profile Index (API) methods. Techniques such as fluorescent antibody (Lotter and Murphy, 1985), polyamine profiling (Auling et al, 1991), 496diamidino-2-phenylindole (DAPI) staining (Streichan et al, 1990), fatty acid analyses of whole-cell hydrolysates (Wagner et al, 1994), and quinone profiling (Hiraishi et al, 1998) were used to estimate abundance of organisms classified as PAOs in sludge samples. Recently, PAOs commonly have been quantified via gene probing coupled with DAPI staining (Crocetti et al, 2000;Hesselmann et al, 1999).…”

Section: Polyphosphate-accumulating Organismsmentioning

confidence: 99%

Research Advances and Challenges in the Microbiology of Enhanced Biological Phosphorus Removal—A Critical Review

Gebremariam

Beutel

Christian

et al. 2011

Water Environment Research

105

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Enhanced biological phosphorus removal (EBPR) is a well-established technology for removing phosphorus from wastewater. However, the process remains operationally unstable in many systems, primarily because there is a lack of understanding regarding the microbiology of EBPR. This paper presents a review of advances made in the study of EBPR microbiology and focuses on the identification, enrichment, classification, morphology, and metabolic capacity of polyphosphate-and glycogen-accumulating organisms. The paper also highlights knowledge gaps and research challenges in the field of EBPR microbiology. Based on the review, the following recommendations regarding the future direction of EBPR microbial research were developed: (1) shifting from a reductionist approach to a more holistic system-based approach, (2) using a combination of culture-dependent and cultureindependent techniques in characterizing microbial composition, (3) integrating ecological principles into system design to enhance stability, and (4) reexamining current theoretical explanations of why and how EBPR occurs. Water Environ. Res., 83, 195 (2011).

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“…However, there is a considerable discrepancy between the numbers of acinetobacters isolated from activated sludge by conventional plate count methods and estimations inferred from direct examinations by chemotaxonomic and immunochemical techniques of wastewater prior to cultivation. On the basis of conventional cultivation methods, acinetobacters represent a major part of the bacteria present in EBPR systems (e.g., 10,12,17), whereas chemotaxonomic and immunochemical data indicate that they may be present only in rather low numbers (4,11,19).…”

mentioning

confidence: 99%

Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge

Wagner

Erhart

Manz

et al. 1994

Appl Environ Microbiol

502

208

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Enhanced biological phosphate removal in an anaerobic-aerobic activated sludge system has generally been ascribed to members of the genus Acinetobacter. A genus-specific 16S rRNA-targeted oligonucleotide probe was developed to investigate the role ofAcinetobacter spp. in situ. Nonisotopic dot blot hybridization to 66 reference strains, including the seven described Acinetobacter spp., demonstrated the expected probe specificity. Fluorescent derivatives were used for in situ monitoring of Acinetobacter spp. in the anaerobic and aerobic compartments of a sewage treatment plant with enhanced biological phosphate removal. Microbial community structures were further analyzed with oligonucleotide probes specific for the alpha, beta, or gamma subclasses of the class Proteobacteria, for the Cytophaga-Flavobacterium cluster, for gram-positive bacteria with a high G+C DNA content, and for all bacteria. Total cell counts were determined by 4',6-diamidino-2-phenylindole staining. In both the anaerobic and the aerobic basins, the activated sludge samples were dominated by members of the class Proteobacteria belonging to the beta subclass and by gram-positive bacteria with a high G+C DNA content. Acinetobacter spp. constituted less than 10% of all bacteria. For both basins, the microbial community structures determined with molecular techniques were compared with the compositions of the heterotrophic saprophytic microbiota determined with agar plating techniques. Isolates on nutrient-rich medium were classified by whole-cell hybridization with rRNA-targeted probes and fatty acid analysis. Cultivation on nutrient-rich medium favored the growth of members of the gamma subclass of Proteobacteria and selected against the growth of members of the beta subclass of Proteobacteria and gram-positive bacteria with a high G+C DNA content; 33% of the cultured bacteria from the anaerobic basin and 32% from the aeration basin were identified as Acinetobacter spp. The addition of small amounts of iron salts for chemical phosphate precipitation had no influence on the constitution of the microbial consortia. Enrichment of the return sludge with 20 mg of acetic acid per liter for 3 days significantly increased the relative abundance of gram-positive bacteria with a high G+C DNA content but had no effect on the numbers of Acinetobacter spp. The dominance of gram-positive bacteria with a high G+C DNA content and the presence of polyphosphate inclusions in these bacteria indicate that they may play a major role in biological phosphate removal.

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Analysis of the polyphosphate-accumulating microflora in phosphorus-eliminating, anaerobic-aerobic activated sludge systems by using diaminopropane as a biomarker for rapid estimation of Acinetobacter spp

Cited by 78 publications

References 32 publications

Evolution of a novel lysine decarboxylase in siderophore biosynthesis

Evolution of a novel lysine decarboxylase in siderophore biosynthesis

Research Advances and Challenges in the Microbiology of Enhanced Biological Phosphorus Removal—A Critical Review

Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge

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