Analysis of the morphology and distribution of argentaffin, argyrophil and insulin-immunoreactive endocrine cells in the small intestine of the adult opossum Didelphis aurita (Wied-Neuwied, 1826)

Basile, D.R.S.; Novaes, Rômulo Dias; Marques, D.C.S.; Fialho, Maria C.Q.; Neves, Clóvis Andrade; Fonseca, Cláudio César

doi:10.1016/j.tice.2012.04.008

Cited by 8 publications

(3 citation statements)

References 20 publications

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“…Left ventricle samples were immersed in a fixative solution (2.5% glutaraldehyde, 0.2% picric acid, 3% sucrose and 5 m m calcium chloride (CaCl 2 ) in 0.1 m sodium cacodylate buffer (pH 7.2)) for 24 h at 4°C for ultrastructural analysis [14]. The samples were postfixed in 1% osmium tetroxide for 2 h and embedding in LR White resin.…”

Section: Methodsmentioning

confidence: 99%

Modulation of oxidative and inflammatory cardiac response by nonselective 1- and 2-cyclooxygenase inhibitor and benznidazole in mice

Santos

Novaes

Bastos

et al. 2015

Journal of Pharmacy and Pharmacology

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BZ triggers an oxidative and nitrosative route, which is associated with increased prostaglandin synthesis and marked damages to the lipids and proteins of the cardiac tissue. IB treatment attenuated reactive stresses triggered by BZ, which was an independent effects of this drug on the endogenous antioxidant enzymes. Individually, but not together, BZ and IB reduced the cardiac inflammatory status, indicating a beneficial and complex drug interaction.

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Section: Methodsmentioning

confidence: 99%

Modulation of oxidative and inflammatory cardiac response by nonselective 1- and 2-cyclooxygenase inhibitor and benznidazole in mice

Santos

Novaes

Bastos

et al. 2015

Journal of Pharmacy and Pharmacology

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show abstract

“…The samples were post-fixed in 1% osmium tetroxide, dehydrated in acetone and embedded in Epon 812 resin. Ultrathin sections were stained with 2% uranyl acetate and 0•2% lead citrate in 1 M sodium hydroxide; and observed with a Zeiss EM 109 transmission electron microscope (Basile et al 2012). Third-order stereology was used to estimate the extent of ultrastructural remodelling in cardiomyocytes.…”

Section: Ultrastructural Analysis and Third-order Stereologymentioning

confidence: 99%

Nonsteroidal anti-inflammatory is more effective than anti-oxidant therapy in counteracting oxidative/nitrosative stress and heart disease inT. cruzi-infected mice

et al. 2017

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We compared the relevance of ibuprofen, vitamins C and E to control oxidative/nitrosative stress and heart disease in mice infected by Trypanosoma cruzi. Swiss mice were randomized into five groups: control, uninfected; infected without treatment; and infected treated with vitamins C, E or ibuprofen. Animals were inoculated with 2000 trypomastigote forms of T. cruzi. After 20 days, infected mice presented reduced vitamin C and E tissue levels, high cytokines (interferon gamma, tumour necrosis factor-α, interleukin 10 and chemokine ligand 2), prostaglandin F2α (PGF2α ) and nitric oxide (NO) cardiac production, intense myocarditis and reactive tissue damage, which was directly correlated with the intensity of the inflammatory infiltrate and the degree of pathological cardiac remodelling. Vitamins C and E supplementation were irrelevant to counteract reactive tissue damage and myocarditis in infected animals. Conversely, ibuprofen reduced tissue levels of cytokines, PGF2α and NO, as well as lipid and protein oxidation, antioxidant enzyme activity and the cardiac damage, without interfering with heart parasitism. Our results do not support the applicability of vitamin C and E supplementation in the management of acute Chagas cardiomyopathy. By controlling the inflammatory infiltrate, anti-inflammatory-based therapy proved to be a more rational strategy than a direct antioxidant therapy in attenuating oxidative/nitrosative stress and cardiac damage.

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“…As células do referido sistema foram denominadas de células claras, argentafins e argirófilas. Tal denominação se deu pela afinidade das mesmas pelos corantes (1) . Atualmente, as células endócrinas do sistema gastroenteropancreático (GEP) são identificadas por técnicas de imunohistoquímica mais especifica (que usam anticorpos para a identificação de antígenos), o que permite a identificação dos mensageiros químicos que secretam (2) .…”

Section: Introductionunclassified

IMUNOLOCALIZAÇÃO DE ENTEROGLUCAGON EM CÉLULAS ENDÓCRINAS PRESENTES NO ESTÔMAGO DO MUÇUÃ <italic>Kinosternon scorpioides</italic> (REPTILIA, CHELONIA, KINOSTERNIDAE)

et al. 2015

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Resumo:O muçuã ou jurará (Kinosternon scorpioides) é um quelônio de pequeno porte de água doce, nativo das varzéas da região amazônica. Essa espécie é importante fonte de alimentação e renda e vem sendo estudada por apresentar vulnerabilidade em consequência da caça indiscriminada, queimadas e desmatamento e, por esta razão, tem sido fonte de pesquisa para o fornecimento de dados para a preservação da espécie. Ainda não é totalmente compreendida a atividade imunohistoquímica das células endócrinas presentes no estômago do muçuã. Sendo assim, este trabalho tem por objetivo identificar a presença do hormônio enteroglucagon e classificar as células endócrinas do estômago de muçuã. Os fragmentos de estômago foram submetidos à técnica de Hematoxilina-Eosina e da Estreptovidina peroxidase para coloração e detecção do antígeno, respectivamente. Foram encontradas células imunorreativas ao enteroglucagon em uma das três porções gástricas (cárdica, fúndica e pilórica); contudo, nas duas primeiras regiões, a imunoreatividade foi mais evidente do que na última. As células endócrinas do estômago de muçuã foram classificadas em argirófilas e argentafins e encontradas entre as células que compõem as glândulas gástricas, sendo classificadas como do "tipo aberta" e do "tipo fechada". Não houve diferença bioquímica e imunohistoquímica entre o enteroglucagon do K. scorpioides com as de outras espécies animais. Palavras-chave: células endócrinas; imunohistoquímica; Kinosternon; tartaruga.

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Analysis of the morphology and distribution of argentaffin, argyrophil and insulin-immunoreactive endocrine cells in the small intestine of the adult opossum Didelphis aurita (Wied-Neuwied, 1826)

Cited by 8 publications

References 20 publications

Modulation of oxidative and inflammatory cardiac response by nonselective 1- and 2-cyclooxygenase inhibitor and benznidazole in mice

Modulation of oxidative and inflammatory cardiac response by nonselective 1- and 2-cyclooxygenase inhibitor and benznidazole in mice

Nonsteroidal anti-inflammatory is more effective than anti-oxidant therapy in counteracting oxidative/nitrosative stress and heart disease inT. cruzi-infected mice

IMUNOLOCALIZAÇÃO DE ENTEROGLUCAGON EM CÉLULAS ENDÓCRINAS PRESENTES NO ESTÔMAGO DO MUÇUÃ <italic>Kinosternon scorpioides</italic> (REPTILIA, CHELONIA, KINOSTERNIDAE)

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