Analysis of the Molecular Mechanism of Acute Coronary Syndrome Based on circRNA‐miRNA Network Regulation

Abstract

Background. With the development of biological technology, biomarkers for the prevention and diagnosis of acute coronary syndrome (ACS) have become increasingly evident. However, the study of novel circular RNAs (circRNAs) in ACS is still in progress. This study aimed to investigate whether the regulation of circRNA-miRNA networks is involved in ACS pathogenesis. Methods. We used microarray analysis to detect significantly expressed circRNAs and miRNAs in the peripheral blood of patients in the control group (CG) and ACS groups, including an unstable angina pectoris (UAP) group and an acute myocardial infarction (AMI) group. A circRNA-miRNA interaction network analysis was carried out with open-source bioinformatics. The gene ontology (GO), pathway, and disease enrichment analyses for differentially expressed circRNAs were further analysed with hierarchical clustering. Results. A total of 266 circRNAs (121 upregulated and 145 downregulated, P<0.05, fold change FC ≥2) and 3 miRNAs (1 upregulated and 2 downregulated, P<0.05, FC ≥ 1.2) were differentially expressed in the ACS groups compared with those in the CG. In addition, among these expressed circRNAs and miRNAs, a single circRNA could bind to more than 1–100 miRNAs, and vice versa. Next, an AMI-UAP network, an AMI-CG network, a UAP-CG network, and an AMI-CG-UAP network were constructed. The top 30 enriched GO terms among the three groups were emphasized as differentially expressed. Disease enrichment analysis showed that these differentially expressed circRNAs are involved in the pathogenesis of cardiovascular diseases. KEGG pathway analysis was performed to identify pathways associated with circRNAs targeting mRNAs. Conclusion. CircRNAs are closely related to the pathological process of ACS via a mechanism that may be related to the up- or down-regulation of circRNAs and miRNAs and circRNA-miRNA coexpression. The metabolic pathways, signalling pathways, and diseases affected by these circRNAs can be predicted by enrichment analysis.

Correlation between Mitochondrial Dysfunction, Cardiovascular Diseases, and Traditional Chinese Medicine

“…TCM has the advantages of multitarget and multipathways [ 42 , 93 ]. A large number of clinical experiences and studies have shown that TCM plays an important role in the prevention, treatment, and prognosis of CVD [ 93 ].…”

“…TCM has the advantages of multitarget and multipathways [ 42 , 93 ]. A large number of clinical experiences and studies have shown that TCM plays an important role in the prevention, treatment, and prognosis of CVD [ 93 ]. Previous studies by our team have shown that circRNAs are closely related to the pathological process of acute coronary syndrome via a mechanism that may be related to the up- or downregulation of circRNAs and miRNAs and the coexpression of circRNA-miRNA [ 94 ].…”

“…Previous studies by our team have shown that circRNAs are closely related to the pathological process of acute coronary syndrome via a mechanism that may be related to the up- or downregulation of circRNAs and miRNAs and the coexpression of circRNA-miRNA [ 94 ]. In addition, through literature review, we consider that the circRNA-miRNA-mRNA network may be a new regulatory mechanism for TCM to effectively treat CVD [ 93 ]. Besides, TCM has been shown to improve the morphology and structure of mitochondria and participate in a series of metabolic processes, including regulation of energy metabolism, inhibition of apoptosis, and reduction of oxygen free radical production in mitochondria.…”

Circular RNAs and Cardiovascular Regeneration

“…With the development of deep sequencing technology, we can now understand the types and differential expression of circRNAs and their associated miRNAs in cardiovascular tissues (101)(102)(103). Many circRNAs have been reported to be associated with CVDs and their expression pattern are different between healthy and diseased human hearts (15,19,104,105).…”

Protein tyrosine phosphatase receptor‑type O expression as a prognostic marker in patients with acute coronary syndrome undergoing percutaneous coronary intervention: A prospective study

“…With the aging of the population and the quickening pace of life, the incidence of acute coronary syndrome (ACS) has continued to increase ( 1 ). ACS is a common cardiovascular disease with a high morbidity and mortality rates ( 2 ).…”