Analysis of the Metaphase Chromosome Karyotypes in Imaginal Discs of Aedes communis, Ae. punctor, Ae. intrudens, and Ae. rossicus (Diptera: Culicidae) Mosquitoes

A karyotypic analysis of three mosquito species Aedes excrucians, Ae. behningi and Ae. punctor (Diptera: Culicidae). Differences in the lengths of chromosomes, the distribution of C- and DAPI blocks of heterochromatin, and the localization of rDNA genes on chromosomes were revealed. Aedes excrucians has the largest chromosome length among the three species represented. Ae. punctor differs in the localization of rDNA on the second chromosome, while in Aedes excrucians and Ae. behningi, rDNA genes are located on chromosome 1. All three species have different C-banding and species-specific localization of heterochromatin DAPI blocks. Consequently, chromosome analysis can serve as an additional mechanism for species identification of mosquitoes of the genus Aedes.

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“…behningi and Ae. punctor has shown that the all three pairs of chromosomes in the examined species are metacentric [6,7].…”

Section: видовая специфика распределения гетерохроматинового блока и mentioning

confidence: 87%

SPECIES SPECIFICITY OF HETEROCHROMATIN BLOCK DISTRIBUTION AND rDNA LOCALIZATION IN MITOTIC CHROMOSOMES OF MOSQUITOES SPECIES AEDES EXCRUCIANS, AE. BEHNINGI AND AE. PUNCTOR

Alekseeva¹,

Andreeva²,

Wasserlauf³

et al. 2020

Conceptual and Applied Aspects of Invertebrate Scientific Research and Biological Education

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“…The previous studies on genome size evaluated the phylogenetic relations in the taxonomy field [6,7,8] ; estimated the completeness of genomes [9] ; and helped in assessing genetic linkage maps [10] . Genome size was usually considered constant within species, and could vary widely between closely related species [27] .…”

Section: Main Bodymentioning

confidence: 99%

The karyotype of the forensically important flesh flies (Diptera: Sarcophagidae), Mini Review

El-Bassiony¹

2022

Preprint

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Maggots of flesh flies were known as one of the main players in the depletion of earthily decomposed remains. They colonized the carrion few minutes after death, and were known as the initial colonizers in several research papers. In consequence, they could be used in the minimum post-mortem intervals (PMImini) estimation. Also, some sarcophagid species proved capable of detecting several toxins/ drugs in carcasses in the entomotoxicological research. The difficulty to identify sarcophagid females and larvae hindered their role as entomological evidence. Cytogenetics would aid in the identification of the immatures and their sex; the differentiation between adult species; and/ or the categorization of the taxa. Sarcophagidae received little attention concerning their karyotype measurements, especially in Egypt where there is only one unpublished thesis (El-Bassiony 2001). This mini review listed the previous studies and findings on sarcophagid’s karyotypes, especially the solitary data from Egypt.

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“…Karyotype analysis can serve as an additional tool in the species level identification of the species, which have morphological similarity and require additional identification methods. Using morphological, molecular, and karyotypic data, more precise species identification can be performed (Alekseeva et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Mitotic metaphase karyotype of the mosquito Anopheles arabiensis Patton (Diptera: Culicidae) from Kassala State, eastern Sudan

Mahmoud Hamza,

Hussein Elboshra

2023

Caryologia

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The mosquito Anopheles arabiensis Patton is the most important malaria vector in Sudan. The study was conducted for the first time to describe numerically the karyotype of An. arabiensis from Kassala State, eastern Sudan. Adults An. arabiensis were caught from human dwellings during the rainy season of 2022. We examined for the first time the utility of brain ganglia tissues of adult mosquitoes for mitotic chromosomal preparations using Giemsa stain - spreading technique. High-quality chromosomal preparations were examined and photographed. Chromosome measurements were carried out using computer software and analyzed statistically using SPSS® software. The diploid mitotic chromosome complement of An. arabiensis consists of three pairs of chromosomes, two pairs of metacentric autosomes (chromosome II and chromosome III) and one acrocentric dot-shaped pair, sex chromosome, which is homomorphic in females (XX) and heteromorphic in males (XY). Chromosome II was described as the longest (2.61±0.07) of the complement and constitute 44.39% of the total length (5.88 μm) of the haploid chromosomes set, while chromosome I (X=1.39 ±0.04; Y=1.04±0.04) as the shortest chromosome. Chromosome X appears in the males significantly larger than chromosome Y (P = 0.00). Chromosome III has an intermediate length (1.88±0.06) compared with the other chromosomes. Comparison of the average lengths of the three chromosome pairs by ANOVA test revealed highly statistical significant differences between them (P < 0.00). The study establishes a strong cytogentic data, which can contribute to accurate identification of the mosquito An. arabiensis and to planning human malaria vector control programs in Kassala State, eastern Sudan.

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Analysis of the Metaphase Chromosome Karyotypes in Imaginal Discs of Aedes communis, Ae. punctor, Ae. intrudens, and Ae. rossicus (Diptera: Culicidae) Mosquitoes

Cited by 3 publications

References 27 publications

SPECIES SPECIFICITY OF HETEROCHROMATIN BLOCK DISTRIBUTION AND rDNA LOCALIZATION IN MITOTIC CHROMOSOMES OF MOSQUITOES SPECIES AEDES EXCRUCIANS, AE. BEHNINGI AND AE. PUNCTOR

SPECIES SPECIFICITY OF HETEROCHROMATIN BLOCK DISTRIBUTION AND rDNA LOCALIZATION IN MITOTIC CHROMOSOMES OF MOSQUITOES SPECIES AEDES EXCRUCIANS, AE. BEHNINGI AND AE. PUNCTOR

The karyotype of the forensically important flesh flies (Diptera: Sarcophagidae), Mini Review

Mitotic metaphase karyotype of the mosquito Anopheles arabiensis Patton (Diptera: Culicidae) from Kassala State, eastern Sudan

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