Analysis of the humoral immune response to Oestrus ovis in ovine

Suárez, J. L.; Scala, Antonio; Romero, J. A.; Paz-Silvạ, A.; Pedreira, J.; Arias, M.; Díaz, Pablo; Morrondo, P.; Díez‐Baños, P.; Sánchez-Andrade, R.

doi:10.1016/j.vetpar.2005.06.009

Cited by 23 publications

(25 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is known that L1 are more discrete, smaller and less nutrient-demanding than L2 or L3 larvae and, as a consequence, they stimulate the immune system less . Nevertheless, specific IgG levels were found to sharply increase and positively interact with developing L2 and/or L3 larvae in sheep and goats (Dorchies & Yilma, 1996;Suárez et al, 2005;Angulo-Valadez et al, 2009a;Romero et al, 2010). Therefore, the IgG response in naturally infected sheep or goats depends on the chronobiology of O. ovis larvae located in a specific area, as well as on particular local conditions (Scala et al, 2002).…”

Section: Systemic Antibody Responsesmentioning

confidence: 99%

“…Systemic involvement of IgA has been found in very low levels in O. ovis-infected sheep, as well as in non-infected animals, showing no relationship with oestrosis infection (Jacquiet et al, 2005;Suárez et al, 2005). It would be interesting to determine if IgA plasma cells in blood are related to oestrosis as IgA participation may be important at the mucosal surface level.…”

Section: Systemic Antibody Responsesmentioning

confidence: 99%

“…Since these reports, research has focused on elucidating how ovine and caprine immune systems attack the larvae and how the larvae modulate these immune reactions in order to survive and develop inside the host. Immune responses against O. ovis have been studied over the last two decades (Dorchies & Yilma, 1996;Duranton et al, 1999;Suárez et al, 2005;Angulo-Valadez et al, 2009a, 2009b and knowledge of the relationships between O. ovis and sheep and goats has progressed considerably. However, the approaches and methods used by oestrosis researchers have been diverse and published data need further analysis and consensus.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sheep and goat immune responses to nose bot infestation: a review

Angulo

Ascencio

Jacquiet

et al. 2010

Medical and Veterinary Entomology

View full text Add to dashboard Cite

Abstract. Oestrus ovis L. (Diptera: Oestridae) is a cosmopolitan agent of myiasis in sheep and goats. The parasitic phase begins after adult females deposit first-stage larvae (L1) into the nostrils of hosts; these larvae develop into L2 and L3 in the nasal and sinus horn cavities. Sneezing and nasal discharges are the major clinical signs in infected animals. The pathogenesis of O. ovis infection is caused by: (a) the trauma resulting from the mechanical action of spines and hooks during larval movement on mucosal membranes, and, more importantly, (b) an allergenic reaction provoked by molecules excreted/secreted by larvae, of which salivary antigens are those mainly recognized by the host's immune system. The recruitment of immune reactive cells increases gradually from the nasal to sinus cavities in infected hosts. Mast cells, eosinophils, macrophages and lymphocytes are always more numerous in infected than non-infected animals. Humoral (antibody) systemic response of immunoglobulin G (IgG) usually reaches seroconversion 2-4 weeks post-first infection and the highest levels are observed during the development of L2 and L3 larvae. Local antibody responses include specific IgG, which has been found to negatively correlate with larval survival and development. Hypersensitivity reaction, immunomodulation, immunization trials and mixed infections of O. ovis and helminths are discussed.

show abstract

Section: Systemic Antibody Responsesmentioning

confidence: 99%

Section: Systemic Antibody Responsesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sheep and goat immune responses to nose bot infestation: a review

Angulo

Ascencio

Jacquiet

et al. 2010

Medical and Veterinary Entomology

View full text Add to dashboard Cite

show abstract

“…Le miasi congiuntivali (od oculari) sono ben documentate in campo veterinario, sia nel Vecchio come nel Nuovo Mondo, vuoi da un punto di vista epidemiologico, vuoi da un punto di vista diagnostico (1,22). In Europa, sempre in campo animale, importanti sono gli attualissimi studi in ambito immunologico e preventivo-profilattico riguardanti ovviamente la popolazione ovina (21,23).…”

Section: Discussioneunclassified

Miasi congiuntivale da Oestrus ovis: descrizione di un nuovo caso e problematiche in tema di miasi in ambito umano

D’Annibale¹,

Crotti²

2007

Microbiol Med

View full text Add to dashboard Cite

INTRODUZIONELe miasi (dal termine greco muia: mosca) sono parassitosi causate da larve di mosche, nella fattispecie da ditteri ciclorrafi appartenenti a diverse famiglie, generi e specie (19). Da un punto di vista epidemiologico le miasi vengono classificate in miasi accidentali, miasi facoltative e miasi obbligatorie (12) Da un punto di vista clinico (e di localizzazione d'organo e/o tessuto) possono venire classificate in miasi cutanee (o superficiali), miasi foruncolose (o sottocutanee), miasi intestinali, miasi cavitarie (tra cui le forme congiuntivali) e miasi profonde o con migrazione interna (12,19). Tutte queste miasi rientrano nelle forme facoltative o profonde (vedi oltre). Le miasi accidentali sono quelle provocate da larve ingerite con alimenti avariati o lavati con acque contaminate. La loro ingestione, peraltro, può essere involontaria (miasi accidentali vere e proprie) o volontaria. Nel primo caso sono coinvolte Musca domestica e Sarcophaga cartaria (25); nel secondo caso essenzialmente Piophila casei, il "verme del formaggio (12,19,20). Clinicamente possono essere responsabili di disturbi gastro-intestinali, anche violenti, ma a prognosi sempre benigna (12,20). Le miasi facoltative sono causate da ditteri che normalmente si nutrono su corpi di animali morti o su materiale organico in decomposizione (nell'uomo su lesioni e ferite purulente o necrotiche) e sul quale facoltativamente possono essere depositate uova o lanciate direttamente le larve (talora anche in cavità naturali, quali, ad esempio, il condotto uditivo esterno o la vagina). Sono coinvolte le seguenti mosche: Lucilia spp. (la "mosca verde"), Phormia spp. (la "mosca nera", europea e nordamericana), Calliphora spp. (la "mosca azzurra"), Chrysomyia spp. (solo in Africa, Asia e Australia), Cochlyomyia spp. (americana), Auchmeromyia spp. (in Africa sub-sahariana), Sarcophaga spp., e Wohlfahrtia spp., quest'ultima presente solo in Europa (9,11,12,19,20). Le prime sei sono ovipare, mentre le ultime due sono larvipare (19). Sono tutte responsabili di miasi cutanee o superficiali, più raramente con coinvolgimento del tessuto sottocutaneo (9, 12). Le miasi obbligatorie comprendono specie parassite obbligatorie che si localizzano allo stadio larvale in tessuti vivi ove compiono una parte del ciclo vitale; le più comuni vie di penetrazione sono le ferite, gli orifizi corporei esterni, la cute anche integra (12,20). Tra le miasi obbligatorie annoveriamo le miasi foruncolose (o, come detto, sottocutanee), le miasi cavitarie, le miasi intestinali vere e proprie, le miasi profonde (12,20). Nelle miasi foruncolose le larve, penetrate attraverso la cute, si sviluppano nello spessore del derma e del sottocutaneo provocando la formazione di un granuloma reattivo (19). Vi appartengono Cordylobia spp. (mosca africana) e Dermatobia spp. (mosca americana). SUMMARYThe Authors related a third case of ophtalmomyasis occurred to a young adult female, during holidays in Sardinia, near a beed of reeds bordering on the beach in the north of the island.Various larv...

show abstract

“…The composition of excretory/secretory antigens is less complex than that of somatic products, so these products have been used for the diagnosis of several parasitic diseases (8,22,24,26). The study presented here analyzes the potential use of protein fractions collected after the incubation of a mixture of cyathostomin third-stage larvae and then run under an automated system of gel exclusion chromatography.…”

mentioning

confidence: 99%

Isolation of Potentially Useful Antigens from Cyathostomin Third-Stage Larvae by Using a Fast Protein Liquid Chromatography One-Step Method

Silva

Francisco

Rodríguez

et al. 2011

Clin. Vaccine Immunol.

Self Cite

View full text Add to dashboard Cite

Three major protein complexes (51, 29, and 15 kDa, named P1 to P3, respectively) were resolved by gel filtration of the excretory/secretory antigens collected from a mixture of horse cyathostomin third-stage larvae (L3s). The potential application for the detection of infected horses was assessed with an enzyme-linked immunosorbent assay (ELISA) by the comparison of the serological and copromicroscopical results. The value of the area under the receiver operating characteristic (ROC) curve was higher than 0.9 when the three peaks were used. Elevated values (>90%) for the sensitivity, specificity, and the positive-likelihood ratio were also observed for all the antigen complexes. A significant increment in the IgG antibody levels 4 weeks prior to the observation of eggs in the feces of weanlings naturally infected was recorded. Our results indicate that the evaluation of chemotherapy is possible by using immunoenzymatic probes and fast protein liquid chromatography (FPLC)-purified antigens. Data collected in the present investigation indicate that FPLC isolation offers a very helpful one-step method for collecting antigens with diagnostic potential to be employed in immunoenzymatic probes.

show abstract

Analysis of the humoral immune response to Oestrus ovis in ovine

Cited by 23 publications

References 17 publications

Sheep and goat immune responses to nose bot infestation: a review

Sheep and goat immune responses to nose bot infestation: a review

Miasi congiuntivale da Oestrus ovis: descrizione di un nuovo caso e problematiche in tema di miasi in ambito umano

Isolation of Potentially Useful Antigens from Cyathostomin Third-Stage Larvae by Using a Fast Protein Liquid Chromatography One-Step Method

Contact Info

Product

Resources

About