Analysis of the HSV-1 strain 17 DNA polymerase gene reveals the expression of four different classes of pol transcripts

Bludau, H.; Freese, Ulrich Karl

doi:10.1016/0042-6822(91)90980-p

Cited by 11 publications

(4 citation statements)

References 59 publications

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“…MiR-H8 is encoded within the second LAT exon, antisense to the first intron of ICP0, consistent with its detection in latently infected ganglia, whereas miR-H14 is encoded within the third exon of ICP0 mRNA, antisense to miR-H2. Interestingly, miR-H11, -H12, and -H13 are encoded within viral origins of replication (oriL and oriS) and could be derived from previously reported transcripts that span the origins (7,28,68). We note that Cui et al predicted that miR-H13 (#6) would be conserved in HSV-2 (15).…”

Section: Resultsmentioning

confidence: 99%

Numerous Conserved and Divergent MicroRNAs Expressed by Herpes Simplex Viruses 1 and 2

Jurak¹,

Kramer²,

Mellor³

et al. 2010

J Virol

144

193

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Certain viruses use microRNAs (miRNAs) to regulate the expression of their own genes, host genes, or both. Previous studies have identified a limited number of miRNAs expressed by herpes simplex viruses 1 and 2 (HSV-1 and -2), some of which are conserved between these two viruses. To more comprehensively analyze the miRNAs expressed by HSV-1 or HSV-2 during productive and latent infection, we applied a massively parallel sequencing approach. We were able to identify 16 and 17 miRNAs expressed by HSV-1 and HSV-2, respectively, including all previously known species, and a number of previously unidentified virus-encoded miRNAs. The genomic positions of most miRNAs encoded by these two viruses are within or proximal to the latencyassociated transcript region. Nine miRNAs are conserved in position and/or sequence, particularly in the seed region, between these two viruses. Interestingly, we did not detect an HSV-2 miRNA homolog of HSV-1 miR-H1, which is highly expressed during productive infection, but we did detect abundant expression of miR-H6, whose seed region is conserved with HSV-1 miR-H1 and might represent a functional analog. We also identified a highly conserved miRNA family arising from the viral origins of replication. In addition, we detected several pairs of complementary miRNAs and we found miRNA-offset RNAs (moRs) arising from the precursors of HSV-1 and HSV-2 miR-H6 and HSV-2 miR-H4. Our results reveal elements of miRNA conservation and divergence that should aid in identifying miRNA functions.

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Section: Resultsmentioning

confidence: 99%

Numerous Conserved and Divergent MicroRNAs Expressed by Herpes Simplex Viruses 1 and 2

Jurak¹,

Kramer²,

Mellor³

et al. 2010

J Virol

144

193

View full text Add to dashboard Cite

show abstract

“…The amounts of primer extension products were quantified by using either a Betagen ,-scope, a Molecular Dynamics PhosphorImager, or a Microtek scanner. In the case ofpol primer extension products, we measured only the closely spaced major products, not the minor (<10%) species (2,26). The ratios of pol mRNA primer extension products to the tk or ICP8 mRNA primer extension products from untreated KOS-infected cells versus drug-treated or mutant virus-infected cells were calculated.…”

Section: Methodsmentioning

confidence: 99%

Unusual regulation of expression of the herpes simplex virus DNA polymerase gene

Wobbe

Digard²,

Staknis

et al. 1993

J Virol

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“…HSV-1 has one and HSV-2 two copies of ori S in each R S , which adds further to the complexity of these miRNAs. A few transcripts that span ori L or ori S have been reported previously [101-103], but these have not yet been investigated in the context of miRNAs. HSV-1 miR-H18 , which has been found in a small number of sequence reads from latently infected ganglia, is encoded upstream of miR-H13 [30].…”

Section: Herpes Simplex Virus 1 and 2 Mirnasmentioning

confidence: 99%

Mammalian alphaherpesvirus miRNAs

Jurak

Griffiths

Coen

2011

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

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Mammalian alphaherpesviruses are major causes of human and veterinary disease. During productive infection, these viruses exhibit complex and robust patterns of gene expression. These viruses also form latent infections in neurons of sensory ganglia in which productive cycle gene expression is highly repressed. Both modes of infection provide advantageous opportunities for regulation by microRNAs. Thus far, published data regarding microRNAs are available for six mammalian alphaherpesviruses. No microRNAs have yet been detected from varicella zoster virus. The five other viruses -- herpes simplex viruses-1 and -2, herpes B virus, bovine herpesvirus-1, and pseudorabies virus -- representing both genera of mammalian alphaherpesviruses have been shown to express microRNAs. In this article, we discuss these microRNAs in terms of where they are encoded in the viral genome relative to other viral transcripts; whether they are expressed during productive or latent infection; their potential targets; what little is known about their actual targets and functions during viral infection; and what little is known about the interactions of these viruses with the host microRNA machinery.

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Analysis of the HSV-1 strain 17 DNA polymerase gene reveals the expression of four different classes of pol transcripts

Cited by 11 publications

References 59 publications

Numerous Conserved and Divergent MicroRNAs Expressed by Herpes Simplex Viruses 1 and 2

Numerous Conserved and Divergent MicroRNAs Expressed by Herpes Simplex Viruses 1 and 2

Unusual regulation of expression of the herpes simplex virus DNA polymerase gene

Mammalian alphaherpesvirus miRNAs

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