Analysis of the expression of BAX, BCL2, BIRC6, CASP3, CASP9 apoptosis genes during the first episode of schizophrenia

Szymona, Kinga; Dudzińska, Ewa; Karakuła-Juchnowicz, Hanna; Gil-Kulik, Paulina; Chomik, Piotr; Świstowska, Małgorzata; Gałaszkiewicz, Joanna; Kocki, Janusz

doi:10.12740/pp/onlinefirst/99971

Cited by 14 publications

(8 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two genes, apoptotic chromatin condensation inducer 1 (ACIN1) and programmed cell death 10 (PDCD10), are involved in apoptosis. Our finding that hypomethylation of cg23712970 in ACIN1 and PDCD10 may result in increased expression of these genes is partially consistent with the recent finding that expression levels of the BCL2 and CASP3 apoptosis genes were significantly higher in the peripheral blood lymphocytes of schizophrenic patients [ 44 ]. Analysis of mRNA expression in the post-mortem brain tissue of patients with SZ also revealed altered mRNA expression in the IFITM2, TIAL1, PDCD6, and MCL genes, which are involved in programmed cell death [ 45 ].…”

Section: Discussionsupporting

confidence: 91%

Methylome-wide Association Study of Patients with Recent-onset Psychosis

Piao

Cui

Rami

et al. 2022

Clin Psychopharmacol Neurosci

View full text Add to dashboard Cite

Objective: Dysregulation of gene expression through epigenetic mechanisms may have a vital role in the pathogenesis of schizophrenia (SZ). In this study, we investigated the association of altered methylation patterns with SZ symptoms and early trauma in patients and healthy controls. Methods: The present study was conducted to identify methylation changes in CpG sites in peripheral blood associated with recent-onset (RO) psychosis using methylome-wide analysis. Lifestyle factors, such as smoking, alcohol, exercise, and diet, were controlled. Results: We identified 2,912 differentially methylated CpG sites in patients with RO psychosis compared to controls. Most of the genes associated with the top 20 differentially methylated sites had not been reported in previous methylation studies and were involved in apoptosis, autophagy, axonal growth, neuroinflammation, protein folding, etc. The top 15 significantly enriched Kyoto Encyclopedia of Genes and Genomes pathways included the oxytocin signaling pathway, long-term depression pathway, axon guidance, endometrial cancer, long-term potentiation, mitogen-activated protein kinase signaling pathway, and glutamatergic pathway, among others. In the patient group, significant associations of novel methylated genes with early trauma and psychopathology were observed. Conclusion: Our results suggest an association of differential DNA methylation with the pathophysiology of psychosis and early trauma. Blood DNA methylation signatures show promise as biomarkers of future psychosis.

show abstract

Section: Discussionsupporting

confidence: 91%

Methylome-wide Association Study of Patients with Recent-onset Psychosis

Piao

Cui

Rami

et al. 2022

Clin Psychopharmacol Neurosci

View full text Add to dashboard Cite

show abstract

“…Additionally, we observed alterations in the expression of genes involved in apoptotic pathways [ 57 ]. The consistent findings in the literature [ 58 ], supported by our group, suggesting that dysregulation of apoptotic mechanisms underlies the molecular basis of SZ, led us to propose anti-apoptotic molecules as a challenging prodromal therapeutic option to halt onset and/or SZ progression.…”

Section: Introductionsupporting

confidence: 76%

Systematic Review of the Therapeutic Role of Apoptotic Inhibitors in Neurodegeneration and Their Potential Use in Schizophrenia

et al. 2022

View full text Add to dashboard Cite

Schizophrenia (SZ) is a deleterious brain disorder affecting cognition, emotion and reality perception. The most widely accepted neurochemical-hypothesis is the imbalance of neurotransmitter-systems. Depleted GABAergic-inhibitory function might produce a regionally-located dopaminergic and glutamatergic-storm in the brain. The dopaminergic-release may underlie the positive psychotic-symptoms while the glutamatergic-release could prompt the primary negative symptoms/cognitive deficits. This may occur due to excessive synaptic-pruning during the neurodevelopmental stages of adolescence/early adulthood. Thus, although SZ is not a neurodegenerative disease, it has been suggested that exaggerated dendritic-apoptosis could explain the limited neuroprogression around its onset. This apoptotic nature of SZ highlights the potential therapeutic action of anti-apoptotic drugs, especially at prodromal stages. If dysregulation of apoptotic mechanisms underlies the molecular basis of SZ, then anti-apoptotic molecules could be a prodromal therapeutic option to halt or prevent SZ. In fact, risk alleles related in apoptotic genes have been recently associated to SZ and shared molecular apoptotic changes are common in the main neurodegenerative disorders and SZ. PRISMA-guidelines were considered. Anti-apoptotic drugs are commonly applied in classic neurodegenerative disorders with promising results. Despite both the apoptotic-hallmarks of SZ and the widespread use of anti-apoptotic targets in neurodegeneration, there is a strikingly scarce number of studies investigating anti-apoptotic approaches in SZ. We analyzed the anti-apoptotic approaches conducted in neurodegeneration and the potential applications of such anti-apoptotic therapies as a promising novel therapeutic strategy, especially during early stages.

show abstract

“…In doing so, dexamethasone inhibits solid tumour and haematologic malignant cell growth, while also suppressing mitogen‐induced proliferation of a wide range of other cell types (Park et al., 2013). Caspase‐3 promotes apoptosis and caspase‐9 plays a signalling and initiation role (Hakem et al., 1998), while Bcl‐2 modulates mitochondrial integrity, functioning as an anti‐apoptotic (Szymona et al., 2019). The present study found that DEXA administration increased caspase‐3 and caspase‐9 expression and decreased immunoreactivity to Bcl‐2 in rats’ testicular tissue.…”

Section: Discussionmentioning

confidence: 99%

Ameliorative impacts ofAllium cepa Linnaeusaqueous extract against testicular damage induced by dexamethasone

et al. 2021

View full text Add to dashboard Cite

The present study aimed to explore the impact of onion (Allium cepa Linnaeus) extract on testicular damage induced by dexamethasone. Forty male Wistar rats were divided into four groups (control, dexamethasone, onion extract and dexamethasone group treated with onion extract). Testosterone levels, antioxidant parameters and the expression of caspase‐3 and IL‐1β, IL‐12, IL‐10 genes, as well as histopathological examination and immunohistochemical studies of Bcl2 and caspase‐9 expression, were examined. Dexamethasone was found to decrease GSH, total antioxidant activity and testosterone levels, meanwhile treatment with onion extract normalised these levels. MDA was increased in dexamethasone group but appeared normal in the treated group. Dexamethasone was shown to downregulate IL‐10 and IL‐2 gene expression. Conversely, IL‐1β and caspase‐3 gene expression were upregulated by dexamethasone and normalised in the treated group. Histopathological analysis found that dexamethasone caused atrophy to the seminiferous tubules and degeneration to spermatocytes, and immunohistochemical analysis showed overexpression of caspase‐9 and inhibited the expression of Bcl‐2 in dexamethasone group. These effects were normalised in the onion extract treated group. In conclusion, onion extract have a preventative effect against dexamethasone‐induced testicular damage in rats; therefore, its use in complementary therapy is recommended.

show abstract

Analysis of the expression of BAX, BCL2, BIRC6, CASP3, CASP9 apoptosis genes during the first episode of schizophrenia

Cited by 14 publications

References 29 publications

Methylome-wide Association Study of Patients with Recent-onset Psychosis

Methylome-wide Association Study of Patients with Recent-onset Psychosis

Systematic Review of the Therapeutic Role of Apoptotic Inhibitors in Neurodegeneration and Their Potential Use in Schizophrenia

Ameliorative impacts ofAllium cepa Linnaeusaqueous extract against testicular damage induced by dexamethasone

Contact Info

Product

Resources

About