Analysis of the Escherichia Coli ATP-Synthase Complex by DNA and Protein Sequencing

Walker, John E.; Eberlé, Alex N.; Hanisch, Peter; Saraste, Matti; Runswick, Michael J.

doi:10.1007/978-1-4612-5832-2_28

Cited by 54 publications

(68 citation statements)

References 36 publications

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“…HPrK\P of M. pneumoniae shares two conserved regions with other members of this class of bifunctional protein kinases\phosphatases : the Walker A box nucleotide-binding site (Walker et al, 1982 ;Saraste et al, 1990 ; residues G154-E162) and the HPrK\P specific signature sequence (Reizer et al, 1998 ; residues E202-V211). We studied the functional importance of these regions by site-directed mutagenesis.…”

Section: Functional Analysis Of Conserved Regions Of Hprk/pmentioning

confidence: 99%

A novel mode of control of Mycoplasma pneumoniae HPr kinase/phosphatase activity reflects its parasitic lifestyle

et al. 2002

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Among the few regulatory proteins encoded by Mycoplasma pneumoniae is HPr kinase/phosphatase (HPrK/P), the key regulator of carbon metabolism in low-GC Gram-positive bacteria. The corresponding gene, hprK, and the gene encoding the target protein HPr, ptsH, were overexpressed. In vitro analysis of the purified proteins confirmed ATP-dependent phosphorylation of HPr by HPrK/P. In contrast to HPrK/P of Bacillus subtilis, which is by default a phosphatase and needs high ATP concentrations for kinase activity, the M. pneumoniae enzyme exhibits kinase activity at very low ATP concentrations and depends on P i for phosphatase activity. This inverted control of enzymic activity may result from the adaptation to very different ecological niches. While the standard activities of HPrK/P from M. pneumoniae and other Grampositive bacteria differ, they are both modulated by the concentration of ATP, P i and glycolytic intermediates. Site-directed mutagenesis of a potential ATPbinding site and of the HPrK/P signature sequence resulted in four different activity classes : (i) inactive proteins, (ii) enzymes with reduced kinase and phosphatase activities, (iii) enzymes that had lost phosphatase, but not kinase activity, and (iv) enzymes that exhibited increased phosphatase activity.

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Section: Functional Analysis Of Conserved Regions Of Hprk/pmentioning

confidence: 99%

A novel mode of control of Mycoplasma pneumoniae HPr kinase/phosphatase activity reflects its parasitic lifestyle

et al. 2002

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“…The ~-subunit of the E.coli enzyme is also required for interaction of bacterial F 1 with the membrane [12]. It binds to the N-terminal region of a-subunits [13] and probably also makes important contacts with the amphiphilic membrane protein b [14]. The amounts of ~ in preparations of bacterial Ft depend upon the method employed for F 1 release; in some preparations it appears to A B C D Fig.I.…”

Section: Introductionmentioning

confidence: 99%

“…Bacterial e-subunit is also important for binding Fl to F0 [17,18]. It appears to interact with the V-subunit [18] and probably with the subunit b [14]. An equivalent role has not been demonstrated for mitochondrial e. Sequence analysis helps to define the relationships between the bacterial and mitochondrial subunits.…”

Section: Introductionmentioning

confidence: 99%

Subunit equivalence in Escherichia coli and bovine heart mitochondrial F₁F₀ ATPases

1982

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“…A more detailed analysis showed that Rep proteins encoded by MMDaV contain the motifs reported in other geminivirus Reps (Fig. S3), which include: (i) the rolling-cycle replication (RCR) motif I (FLTFP), required for specific dsDNA binding; (ii) the RCR motif II (HFH), a metal-binding site that may be involved in protein conformation and DNA cleavage; (iii) the RCR motif III (YIQKE), a catalytic site for DNA cleavage (Nash et al, 2011); (iv) the Walker A (GPT-RSGKT) and the Walker B (LYNVIDDI) domains that are crucial components of the nucleotide-binding site (Walker et al, 1982) (Fig. S3).…”

Section: Y Ma and Others 2426mentioning

confidence: 99%

Identification and molecular characterization of a novel monopartite geminivirus associated with mulberry mosaic dwarf disease

Navarro

Zhang

et al. 2015

Journal of General Virology

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High-throughput sequencing of small RNAs allowed the identification of a novel DNA virus in a Chinese mulberry tree affected by a disease showing mosaic and dwarfing symptoms. Rollingcircle amplification and PCR with specific primers, followed by sequencing of eleven independent full-length clones, showed that this virus has a monopartite circular DNA genome (,2.95 kb) containing ORFs in both polarity strands, as reported previously for geminiviruses. A field survey showed the close association of the virus with diseased mulberries, so we tentatively named the virus mulberry mosaic dwarf-associated virus (MMDaV). The MMDaV genome codes for five and two putative proteins in the virion-sense and in the complementarysense strands, respectively. Although three MMDaV virion-sense putative proteins did not share sequence homology with any protein in the databases, functional domains [coiled-coil and transmembrane (TM) domains] were identified in two of them. In addition, the protein containing a TM domain was encoded by an ORF located in a similar genomic position in MMDaV and in several other geminiviruses. As reported for members of the genera Mastrevirus and Becurtovirus, MMDaV replication-associated proteins are expressed through the alternative splicing of an intron, which was shown to be functional in vivo. A similar intron was found in the genome of citrus chlorotic dwarf-associated virus (CCDaV), a divergent geminivirus found recently in citrus. On the basis of pairwise comparisons and phylogenetic analyses, CCDaV and MMDaV appear to be closely related to each other, thus supporting their inclusion in a putative novel genus in the family Geminiviridae.

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Analysis of the Escherichia Coli ATP-Synthase Complex by DNA and Protein Sequencing

Cited by 54 publications

References 36 publications

A novel mode of control of Mycoplasma pneumoniae HPr kinase/phosphatase activity reflects its parasitic lifestyle

A novel mode of control of Mycoplasma pneumoniae HPr kinase/phosphatase activity reflects its parasitic lifestyle

Subunit equivalence in Escherichia coli and bovine heart mitochondrial F₁F₀ ATPases

Identification and molecular characterization of a novel monopartite geminivirus associated with mulberry mosaic dwarf disease

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Analysis of the Escherichia Coli ATP-Synthase Complex by DNA and Protein Sequencing

Cited by 54 publications

References 36 publications

A novel mode of control of Mycoplasma pneumoniae HPr kinase/phosphatase activity reflects its parasitic lifestyle

A novel mode of control of Mycoplasma pneumoniae HPr kinase/phosphatase activity reflects its parasitic lifestyle

Subunit equivalence in Escherichia coli and bovine heart mitochondrial F1F0 ATPases

Identification and molecular characterization of a novel monopartite geminivirus associated with mulberry mosaic dwarf disease

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Subunit equivalence in Escherichia coli and bovine heart mitochondrial F₁F₀ ATPases