2020
DOI: 10.5194/mr-1-261-2020
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Analysis of the electronic structure of the primary electron donor of photosystem I of <i>Spirodela</i> <i>oligorrhiza</i> by photochemically induced dynamic nuclear polarization (photo-CIDNP) solid-state nuclear magnetic resonance (NMR)

Abstract: Abstract. The electron donor in photosystem I (PSI), the chlorophyll dimer P700, is studied by photochemically induced dynamic nuclear polarization (photo-CIDNP) magic angle spinning (MAS) nuclear magnetic resonance (NMR) on selectively 13C and uniformly 15N labeled PSI core preparations (PSI-100) obtained from the aquatic plant duckweed (Spirodela oligorrhiza). Light-induced signals originate from the isotope-labeled nuclei of the cofactors involved in the spin-correlated radical pair forming upon light excit… Show more

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Cited by 7 publications
(4 citation statements)
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References 86 publications
(101 reference statements)
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“…A comparable observation of emissive light-induced 15 N signals has been reported from the carotenoid-less mutant R. sphaeroides R26, which is known to show a strong DR effect [ 52 ]. Such 1D spectra allow the assignment of the 15 N chemical shift and are based on our preliminary studies on Chl a in PSI [ 53 , 54 ] and PSII [ 73 ], as well as BChl a in R. sphaeroides WT [ 74 ] and R26 [ 52 ]. The 15 N chemical shift assignments are shown in Table 1 .…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…A comparable observation of emissive light-induced 15 N signals has been reported from the carotenoid-less mutant R. sphaeroides R26, which is known to show a strong DR effect [ 52 ]. Such 1D spectra allow the assignment of the 15 N chemical shift and are based on our preliminary studies on Chl a in PSI [ 53 , 54 ] and PSII [ 73 ], as well as BChl a in R. sphaeroides WT [ 74 ] and R26 [ 52 ]. The 15 N chemical shift assignments are shown in Table 1 .…”
Section: Resultsmentioning
confidence: 99%
“…This appears to be a ubiquitous property of ET in all natural photosynthetic RCs [ 48 ]. The effect has been observed in various RC types, such as the type-II RCs of purple bacteria of Rhodobacter ( R. ) sphaeroides wild type (WT) [ 49 , 50 ] and its carotenoid-less mutant R26 [ 51 , 52 ], as well as in PSI and II from plants [ 53 , 54 ] and diatoms [ 55 ]. Also, homodimeric type-I RCs of Hb.…”
Section: Introductionmentioning
confidence: 99%
“…Here, the question of the extremely high redox potential of photosystem II was addressed suggesting a chlorophyll-histidine complex acting as donor [ 90 ]. In photosystem I, the activities of the two branches of cofactors were explored [ 91 ]. Photo-CIDNP MAS NMR allowed signals from 13 C labels introduced into reaction centers to be detected directly from entire plants (Fig.…”
Section: Studies On Photosynthetic Reaction Centers Of Plants Algae and Diatomsmentioning
confidence: 99%
“…A sign change of the solid-state photo-CIDNP effect in the diatom Phaeodactylum tricornutum was also observed at higher magnetic fields and has not yet been rationalized [ 93 ]. Second, sign changes related to selective 13 C isotope incorporation shifting the ratio between enhanced absorptive and emissive intensities in favor of the former have been observed in reaction centers of heliobacteria [ 86 , 99 ] and photosystem I [ 91 ]. Hence, it appears that magnetic 13 C isotopes are not “innocent” but involved into the spin-chemical machinery producing the hyperpolarization.…”
Section: Theory Relying On Level Crossings and Level Anti-crossingsmentioning
confidence: 99%