Photo-CIDNP in Solid State

Matysik, Jörg; Ding, Yong; Kim, Yunmi; Kurle, Patrick; Yurkovskaya, Alexandra V.; Ivanov, Konstantin L.; Alia, A.

doi:10.1007/s00723-021-01322-5

Cited by 14 publications

(19 citation statements)

References 110 publications

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“…In this section, we discuss the occurrence of CIDNP effects in the solid state, in combination with magic angle spinning NMR (photo-CIDNP MAS NMR), which has been recently reviewed. 553 While DNP (sections 3.2, 3.3, and 3.4) relies on the presence of radicals, and ONP (section 3.9) is based on triplet states, CIDNP always requires the presence of radical pairs (RPs) possessing correlated electron spins. The concept of a spincorrelated RP was introduced earlier (sections 3.5 and 3.6).…”

Section: Photochemically Induced Dynamic Nuclear Polarization In the ...mentioning

confidence: 99%

“…CIDNP in the fluid phase was addressed in section , with one notable example being CIDNP in a cyclic photochemical reaction of a flavin dye and an aromatic amino acid in aqueous solution. In this section, we discuss the occurrence of CIDNP effects in the solid state, in combination with magic angle spinning NMR (photo-CIDNP MAS NMR), which has been recently reviewed …”

Section: Hyperpolarization Techniquesmentioning

confidence: 99%

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Spin Hyperpolarization in Modern Magnetic Resonance

et al. 2023

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Magnetic resonance techniques are successfully utilized in a broad range of scientific disciplines and in various practical applications, with medical magnetic resonance imaging being the most widely known example. Currently, both fundamental and applied magnetic resonance are enjoying a major boost owing to the rapidly developing field of spin hyperpolarization. Hyperpolarization techniques are able to enhance signal intensities in magnetic resonance by several orders of magnitude, and thus to largely overcome its major disadvantage of relatively low sensitivity. This provides new impetus for existing applications of magnetic resonance and opens the gates to exciting new possibilities. In this review, we provide a unified picture of the many methods and techniques that fall under the umbrella term "hyperpolarization" but are currently seldom perceived as integral parts of the same field. Specifically, before delving into the individual techniques, we provide a detailed analysis of the underlying principles of spin hyperpolarization. We attempt to uncover and classify the origins of hyperpolarization, to establish its sources and the specific mechanisms that enable the flow of polarization from a source to the target spins. We then give a more detailed analysis of individual hyperpolarization techniques: the mechanisms by which they work, fundamental and technical requirements, characteristic applications, unresolved issues, and possible future directions. We are seeing a continuous growth of activity in the field of spin hyperpolarization, and we expect the field to flourish as new and improved hyperpolarization techniques are implemented. Some key areas for development are in prolonging polarization lifetimes, making hyperpolarization techniques more generally applicable to chemical/biological systems, reducing the technical and equipment requirements, and creating more efficient excitation and detection schemes. We hope this review will facilitate the sharing of knowledge between subfields within the broad topic of hyperpolarization, to help overcome existing challenges in magnetic resonance and enable novel applications.

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Section: Photochemically Induced Dynamic Nuclear Polarization In the ...mentioning

confidence: 99%

Section: Hyperpolarization Techniquesmentioning

confidence: 99%

Spin Hyperpolarization in Modern Magnetic Resonance

et al. 2023

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“…The reader is directed to other reviews for its application on proteins involved in the photosystem, for example, flavoproteins. 24,25 In contrast to previous reviews, in section 3, we aim to give a comprehensive overview of applications in all areas where MAS-DNP provided new biological insight. The years of literature covered range from work on lysozyme in 1997 26 up to the current day, with particular focus from 2017 onward.…”

Section: Scope Of This Reviewmentioning

confidence: 99%

“…The photochemically induced dynamic nuclear polarization (photo-CIDNP) technique is excluded from this review as it requires rather different mechanisms and experimental considerations. The reader is directed to other reviews for its application on proteins involved in the photosystem, for example, flavoproteins. , …”

Section: Introductionmentioning

confidence: 99%

Biomolecular and Biological Applications of Solid-State NMR with Dynamic Nuclear Polarization Enhancement

2022

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Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the investigation of biological systems within their native context, such as lipid membranes, viral capsid assemblies, and cells. However, such ambitious investigations often suffer from low sensitivity due to the presence of significant amounts of other molecular species, which reduces the effective concentration of the biomolecule or interaction of interest. Certain investigations requiring the detection of very low concentration species remain unfeasible even with increasing experimental time for signal averaging. By applying dynamic nuclear polarization (DNP) to overcome the sensitivity challenge, the experimental time required can be reduced by orders of magnitude, broadening the feasible scope of applications for biological solid-state NMR. In this review, we outline strategies commonly adopted for biological applications of DNP, indicate ongoing challenges, and present a comprehensive overview of biological investigations where MAS-DNP has led to unique insights.

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“…1 H-detected 13 C photo-CIDNP can serve as a sensitivity enhancement tool for liquid solution NMR [6,29]. Solid state 1 H, 13 C, and 15 N photo-CIDNP effect can be used to analyze the amino acid tryptophan [30], and served in a new approach using magic angle spinning NMR to analyze photochemical processes in bacterial reaction centers [31][32][33]. Matysik et al [33] provided a detailed overview of how biological solid-state NMR photo-CIDNP enables the study of the dynamics of nuclear spins in photosynthetic reaction centers of bacteria, algae, and higher plants.…”

Section: Abbreviationsmentioning

confidence: 99%

LED-Based Photo-CIDNP Hyperpolarization Enables 19F MR Imaging and 19F NMR Spectroscopy of 3-Fluoro-DL-tyrosine at 0.6 T

et al. 2022

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Although 19F has high potential to serve as a background-free molecular marker in bioimaging, the molar amount of marker substance is often too small to enable 19F MR imaging or 19F NMR spectroscopy with a sufficiently high signal-to-noise ratio (SNR). Hyperpolarization methods such as parahydrogen-based hyperpolarization or dynamic nuclear polarization (DNP) can significantly improve the SNR, but require expensive and complex sample preparation and the removal of toxic catalysts and solvents. Therefore, we used the biologically compatible model of the fluorinated amino acid 3-Fluoro-DL-tyrosine with riboflavin 5ʹ-monophosphate (FMN) as a chromophore dissolved in D2O with 3.4% H2Odest., allowing to transform light energy into hyperpolarization of the 19F nucleus via photo-chemically induced dynamic nuclear polarization (photo-CIDNP). We used a low-cost high-power blue LED to illuminate the sample replacing traditionally used laser excitation, which is both potentially harmful and costly. For the first time, we present results of hyperpolarized 19F MRI and 19F NMR performed with a low-cost 0.6 T benchtop MRI system. The device allowed simultaneous dual-channel 1H/19F NMR. 19F imaging was performed with a (0.94 mm)2 in-plane resolution. This enabled the spatial resolution of different degrees of hyperpolarization within the sample. We estimated the photo-CIDNP-based 19F signal enhancement at 0.6 T to be approximately 465. FMN did not bleach out even after multiple excitations, so that the signal-to-noise ratio could be further improved by averaging hyperpolarized signals. The results show that the easy-to-use experimental setup has a high potential to serve as an efficient preclinical tool for hyperpolarization studies in bioimaging.

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Photo-CIDNP in Solid State

Cited by 14 publications

References 110 publications

Spin Hyperpolarization in Modern Magnetic Resonance

Spin Hyperpolarization in Modern Magnetic Resonance

Biomolecular and Biological Applications of Solid-State NMR with Dynamic Nuclear Polarization Enhancement

LED-Based Photo-CIDNP Hyperpolarization Enables 19F MR Imaging and 19F NMR Spectroscopy of 3-Fluoro-DL-tyrosine at 0.6 T

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