Analysis of the effects of Rosa roxburghii Tratt fruit polyphenols on immune function in mice through gut microbiota and metabolomics: An in vivo preclinical trial study

Liu, Yujie; Zhang, Shuo; Tang, Li; Zhang, Ming; Wang, Pengjiao; Sun, Xiaodong; Shang, Leyuan; Wang, Qi; Zhao, Yanyan; Meng, Dong; Luo, Pei; Xiuli, Gao

doi:10.1016/j.jff.2023.105464

Cited by 8 publications

(5 citation statements)

References 43 publications

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“…After 30 min, the mice were euthanized via cervical dislocation and 2 mL of saline was intraperitoneally injected; then, 1 mL of peritoneal fluid was aspirated after 1 min and smeared on two glass slides followed by incubation at 37°C for 30 min. After complete drying, the smears were stained with Wright's dye for 5 min, dried and sealed with neutral resin, and the number of macrophages were counted under an oil immersion lens of a compound microscope (Liu et al, 2023). The percentage of phagocytosis was analyzed using the following formula:

\begin{matrix} left Phagocytosis percentage ()(%) \\ left goodbreak= \frac{Number of macrophages that had engulfed CRBC}{Number of macrophages} goodbreak\times 100 % \end{matrix}

…”

Section: Methodsmentioning

confidence: 99%

Supplementing maternal diet with milk oligosaccharides and probiotics helps develop the immune system and intestinal flora of offsprings

Xie,

Cui,

Zhu

et al. 2023

Food Science & Nutrition

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Intestinal flora is very important for improving the development of the immune system in newborns. Maternal diet during pregnancy and lactation is one of the key factors affecting the growth and development of offspring. The objective of the present study was to examine whether supplementation of maternal diet with milk oligosaccharides and Bifidobacterium could influence the development of the intestinal flora and immune system of neonatal mice. In total, 30 pregnant Institute of Cancer Research (ICR) mice were randomly divided into six groups: a control group (basal diet) and five intervention groups (basal diet supplemented with different doses of 2′‐fucosyllactose [2′‐FL] and Bifidobacterium Bb12) during the pregnancy period. All female mice were monitored for physical health during gavage. After delivery, the number of mice in each litter, any deformity, and the development of the offspring were recorded. The spleen, blood, and fecal samples of six groups of 10–12 day‐old offspring were collected. The results demonstrated that maternal milk oligosaccharides and probiotics conferred protective effects against lipopolysaccharide (LPS)‐induced immunosuppression in mice offspring by significantly enhancing the immune organ indexes, splenocyte proliferation, immunoglobulin (immunoglobulin G, A, M) production as well as improving the macrophage phagocytosis (p < .05). The abundance of Lactobacilli and Bifidobacteria in the feces of offspring mice in the intervention groups was significantly higher than that of the offspring mice in the control group (p < .05). These findings suggest that the combination of 2′‐FL and Bifidobacterium Bb12 displayed synergistic interactions between the two components that could promote the development of the immune system of the offsprings and improve their microbiota through maternal ingestion.

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\begin{matrix} left Phagocytosis percentage ()(%) \\ left goodbreak= \frac{Number of macrophages that had engulfed CRBC}{Number of macrophages} goodbreak\times 100 % \end{matrix}

…”

Section: Methodsmentioning

confidence: 99%

Supplementing maternal diet with milk oligosaccharides and probiotics helps develop the immune system and intestinal flora of offsprings

Xie,

Cui,

Zhu

et al. 2023

Food Science & Nutrition

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“…Green tea polyphenols enhance host defense against exogenous infections by promoting immune cell proliferation (Shakoor et al., 2021). Another study found that Rosa roxburghii Tratt fruit polyphenols significantly promoted the proliferation of mouse lymphocytes, suggesting improved cellular immune function in normal mice (Liu et al., 2023). Consequently, these results indicated that LJTE reversed the adaptive immunity inhibition in the immunosuppressed mice model by promoting T lymphocyte proliferation.…”

Section: Resultsmentioning

confidence: 99%

Network pharmacology combined with an experimental validation study to reveal the effect and mechanism of Lonicera japonica Thunb. extracts against immunomodulation

Wang,

Pan,

Lin

et al. 2024

Journal of Food Science

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Lonicera japonica Thunb. (LJT) is known for its valuable medicinal properties that highlight its potential application in the pharmaceutical and health food industry. We predict that LJT polyphenols by network pharmacology may be involved in immunomodulation, and the study of LJT polyphenols regulating immunity is still insufficient; therefore, we experimentally found that LJT enhances immunity by promoting the proliferation and phagocytic activity of RAW246.7 cells. A model of an immunosuppressed mouse was constructed using cyclophosphamide‐induced, and LJT was extracted for the intervention. We found that LJT restored immune homeostasis in immune deficiency mice by inhibiting the abnormal apoptosis in lymphocytes, enhancing natural killer cell cytotoxicity, promoting T lymphocyte proliferation, and increasing the CD4+ and CD8+ T lymphocytes in quantity. Moreover, LJT treatment modulates immunity by significantly downregulating lipopolysaccharide‐induced inflammation and oxidative stress levels. We verified the immunomodulatory function of LJT through both cell and animal experiments. The combination of potential‐protein interactions and molecular docking later revealed that LJT polyphenols were associated with immunomodulatory effects on MAPK1; together, LJT intervention significantly modulates the immune, with the activation of MAPK1 as the underlying mechanism of action, which provided evidence for the utilization of LJT as a nutraceutical in immune function.

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“…All analyses were carried out in triplicate. The results were expressed as vitamin gain/loss using the untreated juice as a reference, as presented in Equation (1).…”

Section: Determination Of Vitaminsmentioning

confidence: 99%

“…Functional foods provide health benefits beyond basic nutrition and can be designed to improve one or many functions of the body. These products can help prevent chronic diseases, such as heart disease, cancer, and diabetes; they can improve gut health, reduce the risk of digestive problems, and improve cognitive functions, reducing the risk of age-related cognitive decline; and they have several other health-related benefits [1,2].…”

Section: Introductionmentioning

confidence: 99%

Application of High-Intensity Ultrasound on Acerola (Malpighia emarginata) Juice Supplemented with Fructooligosaccharides and Its Effects on Vitamins, Phenolics, Carotenoids, and Antioxidant Capacity

et al. 2023

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Acerola is considered a superfruit, rich in vitamin C, phenolics, and carotenoids, and having a high antioxidant capacity. However, it is poor in oligosaccharides. Ultrasound technology can improve the bioavailability of several bioactive compounds, improving the nutritional content of several fruit juices. This work evaluated the use of ultrasound processing on acerola juice supplemented with fructooligosaccharides (FOS; 1% w/w) and its effects on the availability of vitamins, carotenoids, and phenolic content. The antioxidant capacity of the juice was correlated with its bioactive contents. The study evaluated the effects of important sonication parameters, such as ultrasonic power density, processing time, and processing temperature. The application of ultrasound was efficient in increasing the availability of some vitamins. As a result, ultrasound application increased the availability of vitamins B1, B3, B5, C, carotenoids, and phenolic compounds. This improvement increased the antioxidant activity of the FOS-enriched acerola juice.

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Analysis of the effects of Rosa roxburghii Tratt fruit polyphenols on immune function in mice through gut microbiota and metabolomics: An in vivo preclinical trial study

Cited by 8 publications

References 43 publications

Supplementing maternal diet with milk oligosaccharides and probiotics helps develop the immune system and intestinal flora of offsprings

Supplementing maternal diet with milk oligosaccharides and probiotics helps develop the immune system and intestinal flora of offsprings

Network pharmacology combined with an experimental validation study to reveal the effect and mechanism of Lonicera japonica Thunb. extracts against immunomodulation

Application of High-Intensity Ultrasound on Acerola (Malpighia emarginata) Juice Supplemented with Fructooligosaccharides and Its Effects on Vitamins, Phenolics, Carotenoids, and Antioxidant Capacity

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