In this paper, a Doherty power amplifier based on an inverse Class-E amplifier with a series output filter are presented. The proposed inverse Class-E amplifier has been designed and simulated using lumped elements and transmission lines (TL), respectively. The high-performance simulation results of TL inverse Class-E PA with an output power of 46.2 dBm, peak drain efficiency of 71%, and a power gain of 27 dB at an input power 18 dBm have been obtained. Finally, the Doherty power amplifier (DPA) based on the inverse Class-E power amplifier with some asymmetry in two paths has been designed and implemented. Implementation of both carrier and peaking amplifiers with an inverse Class-E load network can provide an increase in the efficiency and improvement of harmonic suppression levels. Maximum drain efficiencies of 69.3% and 54% at maximum output power level (50 dBm) and 7-dB back-off power level (43 dBm) have been measured, respectively.