Analysis of the diversity of aerobic, thermophilic endospore-forming bacteria in two Algerian hot springs using cultural and non-cultural methods

Gomri, Mohamed Amine; Khaldi, Tedj El Moulouk; Kharroub, Karima

doi:10.1007/s13213-018-1401-8

Cited by 11 publications

(8 citation statements)

References 94 publications

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“…Isolates are belonging to Bacillales according to their Phenotypic characters. Morphological and microscopic characteristics for the bacterial isolates were similar to the characteristics of the genus Bacillus as was described by De Souza and Martins (2001); Mohammad et al (2017); Gomri et al (2018). Strains of Bacillus were the most studied bacteria, Maugeri et al (2001) isolated 87 aerobic,thermophilic and spore-forming bacteria from Eolian Islands (Italy).…”

Section: Discussionsupporting

confidence: 67%

Potential enzyme activity of thermophilic bacteria from hot spring in Egypt

Taha

El-Shatoury

Tolba

et al. 2020

African Journal of Biological Sciences

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This study aims to isolate and identify thermophilic hydrolytic bacteria from hot spring in South Sinai, Egypt for several industrial applications. In this work, 29 bacterial isolates from hot spring in Egypt were isolated and screened for the production of three thermozymes (amylase, cellulase and protease) at different high temperatures. Fifteen isolates were amylase producer, twenty-one were cellulase producer and twelve isolates were protease producer at different high temperatures. Ten bacterial isolates (34.5%) produced the three extracellular enzymes. All bacterial isolates were identified phenotypically as Bacillus spp. This study concludes that hot springs in Egypt is a source for the isolation of thermophilic bacteria producing thermostable enzymes.

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Section: Discussionsupporting

confidence: 67%

Potential enzyme activity of thermophilic bacteria from hot spring in Egypt

Taha

El-Shatoury

Tolba

et al. 2020

African Journal of Biological Sciences

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“…Moreover, these results also indicate that Algerian hot springs located in the Eastern part of the country harbor a wide variety of thermophilic species, some already known or potentially novel waiting further identification. Interestingly, we notice the isolation and identification of strains belonging to six genera, Aneurinibacillus, Laceyella, Meiothermus, Thermus, Thermobifida, and Saccharomonospora, which have never been reported previously from any hot springs in Algeria [27,28].…”

Section: S21mentioning

confidence: 67%

“…Viksø-Nielsen et al [90] reported a new α-amylase that hydrolyzes raw starch granules. Ecological studies on Algerian hot springs led to the isolation and identification of a large number of thermophilic bacteria capable of producing a mixture of hydrolytic enzymes that may be valuable for biotechnological purposes [28]. Extremophile microorganisms can thrive under extreme conditions of high temperature, salinity, pH, and pressure.…”

Section: G33 24g17mentioning

confidence: 99%

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Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

et al. 2020

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This study aims to determine the diversity of culturable thermophilic bacteria isolated from eight terrestrial hot springs in Northeastern of Algeria using the conventional methods, SDS-PAGE fingerprinting of whole-cell proteins and 16S rRNA gene sequencing. In addition, their hydrolytic enzyme activities were also investigated. A total of 293 strains were isolated from the hot springs' water and sediment using different culture media. Overall, five distinct bacterial groups were characterized by whole-cell protein pattern analysis. Based on the 16S rRNA gene sequencing of 100 selected strains, the isolates were assigned to the following three major phyla: Firmicutes (93%), Deinococcus-Thermus (5%), and Actinobacteria (2%), which included 27 distinct species belonging to 12 different phylotypes, Aeribacillus, Aneurinibacillus, Anoxybacillus, Bacillus, Brevibacillus, Geobacillus, Laceyella, Meiothermus, Saccharomonospora, Thermoactinomyces, Thermobifida, and Thermus. The screening for nine extracellular enzymes showed that 65.87% of the isolates presented at least five types of enzyme activities, and 6.48% of strains combined all tested enzymes (amylase, cellulase, pectinase, esculinase, protease, gelatinase, lipase, lecithinase, and nuclease). It was found that Bacillus, Anoxybacillus, Aeribacillus, and Aneurinibacillus were the genera showing the highest activities. Likewise, the study showed an abundant and diverse thermophilic community with novel taxa presenting a promising source of thermozymes with important biotechnological applications. This study showed that a combined identification method using SDS-PAGE profiles of whole-cell proteins and subsequent 16S rRNA gene sequence analysis could successfully differentiate thermophilic bacteria from Algerian hot springs.

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“…[1] . Heavy metals have been defined and described as "naturally occurring metals having atomic number greater than 20 and an elemental density greater than 5 g cm −3 [2] . Heavy metals are prominent contaminants because they are toxic, non-biodegradable in the environment, and easily accumulated in living organisms [3] .…”

Section: Introductionmentioning

confidence: 99%

The Efficiency of some locally Isolated Fungi on Removing Pb, Cd, Cr and Ni and their Mixture from Wastewater

Montaser

Easa²,

Mansour³

et al. 2022

Egyptian Journal of Pure and Applied Science

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Resources reuse has become an important feature of wastewater management. Wastewater mainly from paint, leather, metal, and tanning industries contain huge amounts of heavy metals. As conventional methods to remove metals from aqueous solutions are not effective enough mainly at low metal concentration and too expensive, alternative methods are required. Microorganisms including fungi have been reported to remove heavy metals from wastewater through bioaccumulation and biosorption at low cost and in eco-friendly way. Aspergillus terreus, A. niger, A. flavus and Talaromyces purpurgenus were isolated from different wastewater samples in Egypt. These isolates exhibited high efficiency in removal of Pb, Cd, Cr and Ni from wastewater, respectively and they can tolerate up to 400 ppm concentration of Pb, Cd, Cr, and Ni. Aspergillus niger showed high efficiency in removing mixture of these heavy metals, this was confirmed by scanning electron microscope coupled with energy dispersive spectroscopy that showed a high amount of metals inside fungal mycelium.

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Analysis of the diversity of aerobic, thermophilic endospore-forming bacteria in two Algerian hot springs using cultural and non-cultural methods

Cited by 11 publications

References 94 publications

Potential enzyme activity of thermophilic bacteria from hot spring in Egypt

Potential enzyme activity of thermophilic bacteria from hot spring in Egypt

Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

The Efficiency of some locally Isolated Fungi on Removing Pb, Cd, Cr and Ni and their Mixture from Wastewater

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