Analysis of the crystallization process in lecithin liposomes: A freeze-etch study

Ververgaert, P.H.J.Th.; Verkleij, Arie J.; Elbers, P. F.; Deenen, L.L.M. Van

doi:10.1016/0005-2736(73)90313-1

Cited by 120 publications

(40 citation statements)

References 14 publications

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“…Fig. 3 gives the results of experiments with liposomes prepared from various saturated leeithins and from some mixtures exhibiting only a single phase transition because of cocrystallization [18][19][20]. Since the light scattering of liposome suspensions changes abruptly on passing the T c [21,22], this criterion could be used to determine the temperature region where the phase transition occurs.…”

Section: Resultsmentioning

confidence: 99%

The effect of chain length and lipid phase transitions on the selective permeability properties of liposomes

Blok

Neut-Kok

Deenen

et al. 1975

Biochimica et Biophysica Acta (BBA) - Biomembranes

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279

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Section: Resultsmentioning

confidence: 99%

The effect of chain length and lipid phase transitions on the selective permeability properties of liposomes

Blok

Neut-Kok

Deenen

et al. 1975

Biochimica et Biophysica Acta (BBA) - Biomembranes

Self Cite

279

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show abstract

“…The 4-maleimido-derivative of Tempo [2,2,6,6-tetramethylpiperidine-l-oxyl, made according to the general procedure described by Griffith and McConnell (18) (19)(20)(21)(22)(23)(24)(25), and these patterns can sometimes be used to distinguish fluid from rigid lipid regions (24)(25)(26). are on the order of 40 A in diameter (uncorrected for shadow thickness).…”

Section: Methodsmentioning

confidence: 99%

Glycophorin in Lipid Bilayers

Grant¹,

McConnell²

1974

Proc. Natl. Acad. Sci. U.S.A.

147

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Glycophorin, the major glycoprotein of human erythrocytes, has been isolated and reincorporated into lipid vesicles. Freeze-fracture electron microscopy shows the reincorporated glycophorin to occur as small particles in vesicle fracture faces while the etch faces are smooth. The glycoprotein has a tendency to cluster into groups of several particles. Evidence is presented that, although lipids in immediate contact with glycophcrin are likely somewhat immobilized, the entire lipid-piotein complex has a tendency to occupy fluid regions of the bilayer. Reincorporated glycophorin assumes its proposed conformation in the intact erythrocyte in so far as it penetrates the hydrophobic membrane interior while its N-terminal end with attached carbohydrate residues is exposed to the aqueous compartment and is available as a specific recognition site.

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“…After I h equilibration at the desired temperature, the samples were quenched and fractured in a Denton Freeze etch machine at 196 °C as described before [3]. The replicas were examined with a Siemens Elmiskop IA.…”

Section: Methodsmentioning

confidence: 99%

“…When the mixture was quenched from 23 °C (i.e. between the two thermotropic peaks [3]) one observes discrete regions with a band pattern, predominantly consisting of dipalmitoylphosphatidylcholine, suspended in the liquid crystalline l-palmitoyl-2-oleoylphosphatidylcholine. When the mixture was quenched from 10 °C, where both species are in the gel state, the individual band pattern of both species is observed.…”

Section: The Influence O[" Cholesterol On the Crystallization Behat mentioning

confidence: 99%

“…It was also shown that, because of this characteristic crystallization behaviour of synthetic phosphatidylcholines, one can visualize a phase separation in phosphatidylcholine mixtures [3]. In an attempt to visualize the distribution of cholesterol in phosphatidylcholine bilayers the crystallization behaviour of (i) a single phosphatidylcholine species, (ii) phosphatidylcholine mixtures that show cocrystallization, and (iii) mixtures that show phase separation, was investigated in the presence of low concentrations of cholesterol.…”

Section: Introductionmentioning

confidence: 99%

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The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturing

Verkleij

Ververgaert

Kruyff

et al. 1974

Biochimica et Biophysica Acta (BBA) - Biomembranes

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SUMMARYThe crystallization behaviour of bilayers of synthetic phosphatidylcholines in the presence of cholesterol was investigated by freeze fracturing.I. Below the lipid-phase transition, cholesterol is randomly distributed over the lateral plane of a bilayer of the single species dimyristoylphosphatidylcholine, as far as can be detected within the lateral resolution of freeze fracturing.2. in an equimolar mixture of dilauroyl-and dimyristoylphosphatidylcholine, which shows cocrystallization, cholesterol is distributed randomly in the bilayers below the phase-transition temperature.3. In an equimolar mixture of dipalmitoyl and l-palmitoyl-2-oleoylphosphatidylcholine, which shows monotectic behaviour, cholesterol interacts preferentially with the liquid crystalline species 1-palmitoyl-2-oleoylphosphatidylcholine when the other component dipalmitoylphosphatidylcholine passes from the liquid crystalline to the gel state upon cooling the mixture.

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Analysis of the crystallization process in lecithin liposomes: A freeze-etch study

Cited by 120 publications

References 14 publications

The effect of chain length and lipid phase transitions on the selective permeability properties of liposomes

The effect of chain length and lipid phase transitions on the selective permeability properties of liposomes

Glycophorin in Lipid Bilayers

The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturing

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