The effect of chain length and lipid phase transitions on the selective permeability properties of liposomes

Blok, M.C.; Neut-Kok, E.C.M. Van Der; Deenen, L.L.M. Van; Gier, J. De

doi:10.1016/0005-2736(75)90003-6

Cited by 279 publications

(90 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 4A shows the release of K ÷ from multilayered liposomes of DMPC and DMPE as a function of temperature. As shown before the liposomes of DMPC loose all their enclosed K + when incubated at temperatures in the vicinity of the gel to liquid-crystalline phase transition [1]. In contrast, such an increase in permeability could not be noticed in liposomes of DMPE.…”

Section: Raman Studiessupporting

confidence: 50%

“…For pure lecithin bilayers it has been demonstrated that ionic permeability [1][2][3], susceptibility for pancreatic phospholipase A2 degradation [4] and rate of transbilayer movement of lipid molecules [5] are markedly increased in the temperature range at which the hydrocarbon phase transition occurs. Two possible explanations have been given for the enhanced permeability in the vicinity of the gel to liquidcrystalline phase transition temperature.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative study on the properties of saturated phosphatidylethanolamine and phosphatidylcholine bilayers: Barrier characteristics and susceptibility to phospholipase A2 degradation

Noordam

Killian

Elferink

et al. 1982

Chemistry and Physics of Lipids

Self Cite

View full text Add to dashboard Cite

Comparative studies on bilayer systems of saturated phosphatidylcholines and phosphatidylethanolamines revealed a maximum in ionic permeability in phosphatidylcholine bilayers at the temperature of the gel to liquid-crystalline phase transition but such an increase in permeability was not detectable in bilayers of phosphatidylethanolamine. Furthermore, it was found that at the phase transition temperature the phosphatidyicholine bilayers are subject to rapid hydrolysis by pancreatic phospholipase A2 whereas phosphatidylethanolamine bilayers are not. These differences are discussed in view of detailed information on the molecular organization in the gel and liquid crystalline phases of the two phospholipid classes.

show abstract

Section: Raman Studiessupporting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Comparative study on the properties of saturated phosphatidylethanolamine and phosphatidylcholine bilayers: Barrier characteristics and susceptibility to phospholipase A2 degradation

Noordam

Killian

Elferink

et al. 1982

Chemistry and Physics of Lipids

Self Cite

View full text Add to dashboard Cite

show abstract

“…Solute leakage from liposomes rapidly accelerates when the components which constitute the liposomes cause phase separation. 15,18) However, CF leakage from liposomes in the gel state decreased with increasing mole fractions of DSPE-PEG, and was suppressed even for all PEG molecular weights. Therefore, in the gel state, the effect of phase separation on CF leakage can be decreased by decreasing the mobility of lipid molecules which constitute the liposomal bilayer membrane.…”

Section: Resultsmentioning

confidence: 97%

Effects of Poly(ethylene glycol) (PEG) Concentration on the Permeability of PEG-Grafted Liposomes

Hashizaki

Taguchi

Itoh

et al. 2005

CHEMICAL & PHARMACEUTICAL BULLETIN

View full text Add to dashboard Cite

Liposomal drug delivery systems (DDS) have been widely researched for use in drug toxicity reduction and/or drug targeting to desired target tissues.1) However, liposomes are rapidly removed from the circulation following their intravenous administration primarily by Kupffer cells in the liver and fixed macrophages in the spleen. Thus, it is important to develop modified liposomes that are able to avoid uptake by the reticuloendothelial system (RES) and extend their circulation half-life in vivo. Many studies have reported that the conjugation of amphipathic polyethylene glycol (PEG) with liposomes (PEG-liposomes) significantly increases the blood circulation liposome half-life compared with those without PEG. [2][3][4][5] In our previous studies, 6,7) we reported on the effect of PEG-lipid PEG chain length on the membrane characteristics of liposomes. The addition of PEG-lipid to liposomes caused lateral phase separation in the gel and liquid-crystalline states, and the fluidity in the interfacial region of liposomal bilayer membranes was markedly increased by the addition of PEG-lipids. In addition, the permeability of liposomal bilayer membranes decreased compared with those without PEG. From these results, we concluded that the solute permeability of PEG-liposomes is affected by not only the properties of the liposomal bilayer membranes such as phase separation and membrane fluidity, but also the PEG chain length of the liposomal surface.In this study, we investigated the effects of PEG-lipid concentration on CF leakage from PEG-liposomes to elucidate the permeation mechanism of PEG-liposomes in more detail. ExperimentalMaterials L-a -Dipalmitoylphosphatidylcholine (DPPC, 99.6% pure) was obtained from NOF Co., Ltd., and Distearoyl-N-monomethoxy poly-(ethylene glycol)-succinyl-phosphatidylethanolamines (DSPE-PEG) were acquired from NOF Co., Ltd. (Tokyo, Japan). The weight-average molecular weights of poly(ethylene glycol) were 1000, 2000, 3000 and 5000. 5-(6)-Carboxyfluorescein (CF, 99% pure) was purchased from Molecular Probes, Inc. (OR, U.S.A.) and was used without further purification. 1,6-Diphenyl-1,3,5-hexatriene (DPH, 98% pure) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH, 95% pure) were purchased from Sigma Chemical Co. (MO, U.S.A.) and were also used without further purification. Dulbecco's Phosphate-buffered saline (PBS) powder composed of NaCl, KCl, Na 2 HPO 4 and KH 2 PO 4 was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). PBS powder was dissolved in water for injection (Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan), and an isotonic solution of pH 7.4 was used. All other chemicals were commercial products of reagent grade.Preparation of PEG-Liposomes PEG-liposomes were prepared using DPPC and DSPE-PEG, which were first dissolved in chloroform in a test tube. The solvent was then removed by blowing nitrogen gas into the test tube, and the residual solvent was further dried overnight at room temperature in a desiccator under a vacuum. PBS was added to the ...

show abstract

“…Under physiological conditions, the immobile phospholipid fraction was immobilized to the cytoskeleton or to the extracellular matrix. The lateral diffusion properties of the lipid bilayer depended on the lipid content (Blok et al, 1975;Cullis, 1976): Both the amount of cholesterol and the head group composition of the phospholipids, as well as the acyl composition determine the fluidity of the membrane. Below the so called phase transition temperature, the fluid lipids will enter a frozen state in which they do not show lateral diffusion (Feigenson, 1976).…”

Section: Detection Of Lateral Diffusionmentioning

confidence: 99%

Sperm head membrane reorganisation during capacitation

Gadella¹,

Tsai²,

Boerke³

et al. 2008

Int. J. Dev. Biol.

171

150

View full text Add to dashboard Cite

The sperm cell has a characteristic polarized morphology and its surface is also highly differentiated into different membrane domains. Junctional protein ring structures seal the surface of the mid-piece from the head and the tail respectively and probably prevent random diffusion of membrane molecules over the protein rings. Despite the absence of such lateral diffusion-preventing structures, the sperm head surface is also highly heterogeneous. Furthermore, lipid and membrane protein ordering is subjected to changes when sperm become capacitated. The forces that maintain the lateral polarity of membrane molecules over the sperm surface, as well as those that cause their dynamic redistribution, are only poorly understood. Nevertheless, it is known that each of the sperm head surface regions has specific roles to allow sperm to fertilize the oocyte: a specific region is devoted to zona pellucida binding, a larger area of the sperm head surface is involved in the acrosome reaction (intracellular fusion), while yet another region is involved in egg plasma membrane binding and fertilization fusion (intercellular membrane fusion). All three events occur in the area of the sperm head where the plasma membrane covers the acrosome. Recently, lipid ordered microdomains (lipid rafts) were discovered in membranes of many biological specimens including sperm. In this review, we cover the latest insights about sperm lipid raft research and discuss how sperm lipid raft dynamics may relate to sperm-zona binding and the zona-induced acrosome reaction.

show abstract

The effect of chain length and lipid phase transitions on the selective permeability properties of liposomes

Abstract: SUMMARYThis paper describes experiments showing the importance of the fatty acid chain length on the barrier properties of liposomal bilayers, prepared from saturated lecithins, under conditions of lateral phase separation.

Cited by 279 publications

References 25 publications

Comparative study on the properties of saturated phosphatidylethanolamine and phosphatidylcholine bilayers: Barrier characteristics and susceptibility to phospholipase A2 degradation

Comparative study on the properties of saturated phosphatidylethanolamine and phosphatidylcholine bilayers: Barrier characteristics and susceptibility to phospholipase A2 degradation

Effects of Poly(ethylene glycol) (PEG) Concentration on the Permeability of PEG-Grafted Liposomes

Sperm head membrane reorganisation during capacitation

Contact Info

Product

Resources

About