Analysis of the Contribution of CTL Epitopes in the Immunobiology of Morbillivirus Infection

Beauverger, Philippe; Cardoso, Alicia I.; Daviet, Laurent; Buckland, Robin; Wild, T. F.

doi:10.1006/viro.1996.0230

Cited by 13 publications

(10 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CTL response primed by buccal immunization with NP is mediated by L d -restricted CD8 ϩ T cells, which recognize the dominant L d epitope NP 281-289 , similarly to CTL induced by s.c. injection of NP or by tail scarification with a recombinant VV encoding NP (VV-NP) (27). This suggests that the same MHC class I-processing pathway of NP operates in APC of buccal mucosa and skin.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the CTL response induced by buccal immunization with NP is able to protect mice against a lethal intracerebral challenge with CDV, which shares with MV the unique NP 281-289 L d epitope. Previous studies have shown that vaccination with VV-NP or with a VV recombinant encoding the NP 281-289 inserted into the gene encoding for CD36 generated NP-specific CTL and completely protected mice against CDV challenge; alternatively, a VV-NP recombinant that has a mutation in the consensus sequence of NP 281-289 did not induce CTL and was unable to protect mice against CDV challenge (27). We observed that all mice immunized by buccal injection of NP survived a lethal CDV challenge.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated that NP-specific CTL induced by parenteral immunization with recombinant VV encoding a class I epitope of MV-NP can protect mice against lethal challenge with a neuroadapted strain of CDV, a Morbillivirus whose NP shares the same dominant L d epitope (27). We took advantage of this model to examine the protective role of NP-specific CTL generated by buccal immunization with recombinant NP.…”

Section: Buccal Immunization With Recombinant Np Protects From Lethalmentioning

confidence: 99%

See 2 more Smart Citations

Dendritic Cells Recruitment and In Vivo Priming of CD8+ CTL Induced by a Single Topical or Transepithelial Immunization Via the Buccal Mucosa with Measles Virus Nucleoprotein

Etchart¹,

Desmoulins²,

Chemin³

et al. 2001

The Journal of Immunology

View full text Add to dashboard Cite

The buccal mucosa, a prototype of pluristratified mucosal epithelia, contains a network of directly accessible class II+ epithelial dendritic cells (DC), similar to skin Langerhans cells. We showed that a single buccal immunization with measles virus nucleoprotein (NP), by either topical application onto or intradermal injection in the buccal mucosa, induced in vivo priming of protective class I-restricted specific CD8+ CTL. Both routes of immunization with NP induced a rapid recruitment of DC into the mucosa, which peaked at 2 h and decreased by 24 h. Treatment of mice with Flt3 ligand resulted in an increased number of DC in the buccal mucosa and enhanced the frequency of IFN-γ-producing NP-specific effectors and the NP-specific CTL response generated after buccal immunization with NP. Finally, NP-pulsed bone marrow-derived DC induced NP-specific IFN-γ-producing cells upon adoptive transfer to naive mice. These data demonstrate that a viral protein delivered to DC of the buccal mucosa induces in vivo priming of protective anti-viral CD8+ CTL.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Buccal Immunization With Recombinant Np Protects From Lethalmentioning

confidence: 99%

See 1 more Smart Citation

Dendritic Cells Recruitment and In Vivo Priming of CD8+ CTL Induced by a Single Topical or Transepithelial Immunization Via the Buccal Mucosa with Measles Virus Nucleoprotein

Etchart¹,

Desmoulins²,

Chemin³

et al. 2001

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…CDV infection had been well controlled by vaccination. However, epidemics of CDV infection have been appeared in the last decade even in vaccinated dogs [6,7].Cytotoxic T lymphocytes (CTL) play an important role in the immune system for the clearance and protection against morbilliviruses [3,22]. CTL epitopes and responses to measles virus (MV) infection have been well studied in a mouse model.…”

mentioning

confidence: 99%

“…Cytotoxic T lymphocytes (CTL) play an important role in the immune system for the clearance and protection against morbilliviruses [3,22]. CTL epitopes and responses to measles virus (MV) infection have been well studied in a mouse model.…”

mentioning

confidence: 99%

Cytotoxic T-Lymphocyte Activity Specific for Hemagglutinin (H) Protein of Canine Distemper Virus in Dogs.

Hirama

Togashi

Wakasa

et al. 2003

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Cytotoxic T-lymphocyte (CTL) responses to hemagglutinin (H) protein of canine distemper virus (CDV) were evaluated in dogs using the replication-deficient adenovirus protein expression system. Skin fibroblasts were isolated from two dogs and were infected with recombinant adenovirus bearing the CDV-H gene (Ade-CDVH). CTL assay was performed using fibroblasts expressing CDV-H protein as target cells and peripheral blood lymphocytes (PBL) collected from the same dogs one week after immunization of CDV as effector cells. Specific cytotoxic activity was observed against autologous but not heterologous fibroblasts expressing Canine distemper virus (CDV) belongs to the genus Morbillivirus family Paramyxoviridae and induces fatal diseases including encephalitis with demyelination, diarrhea and respiratory disorders in dogs. CDV infection had been well controlled by vaccination. However, epidemics of CDV infection have been appeared in the last decade even in vaccinated dogs [6,7].Cytotoxic T lymphocytes (CTL) play an important role in the immune system for the clearance and protection against morbilliviruses [3,22]. CTL epitopes and responses to measles virus (MV) infection have been well studied in a mouse model. The major target antigen for CTL in mice was identified as the nucleocapsid (N) protein by several groups, which can induce cross reactive CTL between MV and CDV [1-3, 15, 19]. In addition, it was also reported that H protein of MV [1] and CDV [21] could be one of the CTL epitopes in mice. However, Jaye et al. reported that the fusion (F) and hemagglutinin (H) proteins were important targets for measles CTL responses in humans, a natural host of MV, receiving measles polypetides [11]. On the other hand, CTL responses in infection with another morbillivirus, rinderpest virus (RPV), were also analyzed in mice and cattle, the latter of which is a natural host of RPV. A CTL response against RPV N protein was detected and a CTL epitope within the N protein was identified in the mouse model [13]. However, the role of N protein in protection and induction of CTL responses against RPV infection in cattle was limited [16]. The CTL response against H protein in cattle is more effective and its effect lasts for at least two years [17,20]. These discrepant results suggested that the CTL response in the mouse model does not always reflect that in the natural host, and analyses concerning the development of CTL activity using the virus and the natural host are required.Restriction of the major histocompatibility complex (MHC) in CTL assay makes it difficult to develop an assay system in outbred animals. Recently, an easy method to produce recombinant adenoviruses based on a replicationdeficient adenovirus vector was established, and this method has been shown to be useful for delivery of vectors for gene therapy and protein expression [12,14]. The adenovirus vector possesses useful characteristics such as high level of protein expression, broad host cell range, applicability to non-replicating cells including neu...

show abstract

Identification of a Cytotoxic T-Cell Epitope on the Recombinant Nucleocapsid Proteins of Rinderpest and Peste des petits ruminants Viruses Presented as Assembled Nucleocapsids

2001

View full text Add to dashboard Cite

The nucleocapsid protein (N) of morbilliviruses is not only a major structural protein but also the most abundant protein made in infected cells. We overexpressed the N proteins of Rinderpest virus and Peste des petits ruminants virus in E. coli, which assemble into nucleocapsids in the absence of viral RNA that resemble nucleocapsids made in the virus-infected cells. Employing these assembled structures resembling subviral particles, we studied the induction of both the antibody response and the cytotoxic T-lymphocyte (CTL) response in a murine model (BALB/c). A single dose of the purified recombinant nucleocapsids of both viruses in the absence of an adjuvant induces a strong CTL response. The CTLs generated are antigen specific and cross-reactive with respect to each virus and, furthermore, this CTL response is MHC class I restricted. Based on the prediction for H-2(d)-restricted T-cell motifs we tested the lysis of transfected P815 (H-2(d)) cells expressing a nine amino acid potential CTL epitope, by splenic T cells in vitro restimulated with bacterially expressed RPV or PPRV N proteins. We extended our study to the bovine system both to analyze the immunogenicity of these recombinant proteins in the natural hosts and to show that PBMC from cattle vaccinated with Rinderpest vaccine proliferate in vitro, in response to restimulation with soluble nucleocapsid proteins. Furthermore, the murine CTL epitope functions in the bovine system as a cytotoxic T-cell epitope. This sequence, which is conserved in the N proteins of morbilliviruses, conforms well to the predicted algorithm for some of the most common BoLA CTL antigenic peptides.

show abstract

Analysis of the Contribution of CTL Epitopes in the Immunobiology of Morbillivirus Infection

Cited by 13 publications

References 5 publications

Dendritic Cells Recruitment and In Vivo Priming of CD8+ CTL Induced by a Single Topical or Transepithelial Immunization Via the Buccal Mucosa with Measles Virus Nucleoprotein

Dendritic Cells Recruitment and In Vivo Priming of CD8+ CTL Induced by a Single Topical or Transepithelial Immunization Via the Buccal Mucosa with Measles Virus Nucleoprotein

Cytotoxic T-Lymphocyte Activity Specific for Hemagglutinin (H) Protein of Canine Distemper Virus in Dogs.

Identification of a Cytotoxic T-Cell Epitope on the Recombinant Nucleocapsid Proteins of Rinderpest and Peste des petits ruminants Viruses Presented as Assembled Nucleocapsids

Contact Info

Product

Resources

About