Analysis of the anti-inflammatory and chemopreventive potential and description of the antimutagenic mode of action of the<i>Annona crassiflora</i>methanolic extract

Rocha, Roberta Schroder; Kassuya, Cândida Aparecida Leite; Formagio, Anelise Samara Nazari; Mauro, Mariana de Oliveira; Andrade-Silva, Magaiver; Monreal, Antônio Carlos Duenhas; Cunha-Laura, Andréa Luiza; Vieira, Maria do Carmo; Oliveira, Rodrigo Juliano

doi:10.3109/13880209.2015.1014567

Cited by 30 publications

(19 citation statements)

References 59 publications

(61 reference statements)

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“…In the pre-treatment protocol (desmutagenic effect), a protective effect was detected at all evaluated concentrations in A. cepa. It is likely that the phytochemicals of AEBPb interacted with MMS, demonstrating the efficiency of AEBPb in adsorbing and/or chemically inactivating MMS as observed by Rocha et al (2016) in cells of A. cepa. However, in mice, the protective effect (pre-treatment) was observed only at higher doses (20 and 40 mg/kg), indicating that the metabolism and bioavailability of AEBPb occurred more efficiently only after 72 h. This result evidences that the phytochemicals of AEBPb also acted on the inhibition of mutagens arising from the metabolism of cyclophosphamide, inhibiting its uptake or blocking its interaction with the DNA (Araldi et al, 2015).…”

Section: Discussionmentioning

confidence: 94%

“…The importance of antioxidative compounds, such as phenolic compounds, could be related to the inhibition of free radical formation (Rocha et al, 2016). In excess, these radicals may cause damage to the DNA, proteins, mitochondria and membranes, promoting alterations in cell structure and functions, and are therefore involved in pathologies, including cancer (Mendes, Costa, & Mateus, 2015).…”

mentioning

confidence: 99%

“…Antimutagenic agents may be classified as either desmutagenic or bio-antimutagenic. The first acts directly on the compounds that induce mutations in the DNA and bio-antimutagenic agents act mainly in the repair of DNA lesions (Rocha, et al, 2016).…”

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confidence: 99%

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Antimutagenic activity and identification of antioxidant compounds in the plant Poincianella bracteosa (Fabaceae)

Couto

Araújo

Lopes

et al. 2019

RBT

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Poincianella bracteosa (Tul.) L.P. Queiroz. (Fabaceae), known as catingueira, is traditionally used in medicine to treat catarrhal infections, diarrhea, hepatitis and anemia. However, there are no studies on toxicogenetics effects of P. bracteosa. The present study aimed to investigate the phytochemical profile as well as the mutagenic and antimutagenic potential of P. bracteosa aqueous barks extract in Allium cepa and Mus musculus. Barks from P. bracteosa were collected in Teresina (PI, Brazil) and male Swiss mice (Mus musculus) were provided by the vivarium from the Faculty of Medical Sciences (FACIME) (PI, Brazil). All procedures using animals were approved by the Ethics Committee on the Use of Animals (Protocol No. 07557/15) with five groups (five animals per group). The extract from barks was diluted in distilled water to yield the four concentrations (2, 4, 8 and 16 mg/mL) used in the A. cepa bioassay and the three doses (10, 20 and 40 mg/Kg) administered to the mice. The phytochemical profile was performed by the colorimetric test to identify the main secondary metabolites in the barks extract. After treatment, the roots of A. cepa were washed in distilled water and hydrolyzed (60°C) for 10 min. After hydrolysis, the roots were washed in distilled water and stained with Schiff's reagent (2 h) in the dark. Five thousand meristematic cells (ten slides) were analyzed to determine the mitotic index, the mean number of chromosome alterations and the percentage of damage reduction. For mice, after 24, 48 and 72 h, the blood of tail of each animal was collected for the preparation of two slides per animal. Slides were dried (24 h), fixed in methanol (5 min.), stained with Giemsa (15 min.) and washed with distilled water. For each animal assay, 2,000 normochromatic erythrocytes per mice were evaluated to establish the number of micronuclei and the protective effect. The data were analyzed by Kruskal-Wallis test (p < 0.05). Phytochemical study of the extract detected reducing sugars and tannins. Probably, the phytochemicals in the extract did not interfere with the cell cycle (A. cepa) nor caused damage to the DNA (A. cepa and mice), and exhibited protective effect in both studied species. The observed data indicate the importance of P. bracteosa bark extract for the inhibition of damage and chemoprevention. However, more studies should be carried out to ensure its protective effect on the genetic material.

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Section: Discussionmentioning

confidence: 94%

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confidence: 99%

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Antimutagenic activity and identification of antioxidant compounds in the plant Poincianella bracteosa (Fabaceae)

Couto

Araújo

Lopes

et al. 2019

RBT

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“…The main protocols in studies of antimutagenic modes of action are pre-treatment and simultaneous treatment, which indicate both desmutagenesis and bioantimutagenesis activity; simultaneous treatment with preincubation, which indicates desmutagenesis activity; and post-treatment, which indicates bioantimutagenesis activity (Oliveira et al, 2006(Oliveira et al, , 2007. These protocols are frequently used in pre-clinical experiments (Fedel-Miyasato et al, 2014a,b;Cantero et al, 2015;Navarro et al, 2015;Oliveira et al, 2015;Magosso et al, 2016;Martello et al, 2016;Rocha et al, 2016;Schneider et al, 2016), and have been further adapted for the A. cepa assay (Fedel-Miyasato et al, 2014a;Felicidade et al, 2014a,b;Mauro et al, 2014;Nantes et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…In all of the experimental protocols, seeds were grown for 24 h in distilled water followed by 96 h under treatment. For the antimutagenesis evaluation, simultaneous, pre-treatment, and post-treatment protocols were employed (Rocha et al, 2016), as presented in Figure 1. After treatment, the roots were collected and fixed in Carnoy's solution for at least 6 h. They were then subjected to acid hydrolysis (1 N) and Schiff-stained for 2 h in the dark.…”

Section: Experimental Designmentioning

confidence: 99%

Moquiniastrum polymorphum subsp floccosum extract: screening for mutagenic and antimutagenic activity

Oliveira¹,

David²,

Pesarini³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Moquiniastrum polymorphum subsp floccosum (Cabrera) 2 R.J. Oliveira et al. Genetics and Molecular Research 15 (4): gmr15048976 G. Sancho is used in traditional Brazilian medicine to treat inflammation and infection, which is supported by scientific data. However, only one study has been conducted on the mutagenic activity of the extract, which has important safety implications. This study evaluated the mutagenic/antimutagenic activity of M. polymorphum ethanolic extract (MPEE) in Allium cepa meristematic cells. Commercial A. cepa seeds were cultured for 120 h. Treatments were performed for 48 h with MPEE (10 mg/mL), methyl methanesulfonate (MMS; 0.01 mg/mL), or in combination (MPEE + MMS). All of the experiments were performed in triplicate. A total of 15,000 cells per treatment were analyzed for chromosomal aberrations and the mitotic index. The results showed that MPEE was not mutagenic. In combination with MMS, MPEE decreased the number of damaged cells and the mitotic index. Interestingly, the most pronounced effect was observed post-treatment when the mitotic index also decreased, suggesting that MPEE may affect the cell cycle. MPEE exhibited antimutagenic activity, and may induce cell cycle arrest in A. cepa.

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Annonaceae: Tropical Medicinal Plants with Potential Anticancer Acetogenins and Alkaloids

Joseph,

Amala Dev

2023

Bioprospecting of Tropical Medicinal Plants

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Analysis of the anti-inflammatory and chemopreventive potential and description of the antimutagenic mode of action of theAnnona crassifloramethanolic extract

Abstract: These results suggest that this medicinal plant has chemopreventive and anti-inflammatory therapeutic potential.

Cited by 30 publications

References 59 publications

Antimutagenic activity and identification of antioxidant compounds in the plant Poincianella bracteosa (Fabaceae)

Antimutagenic activity and identification of antioxidant compounds in the plant Poincianella bracteosa (Fabaceae)

Moquiniastrum polymorphum subsp floccosum extract: screening for mutagenic and antimutagenic activity

Annonaceae: Tropical Medicinal Plants with Potential Anticancer Acetogenins and Alkaloids

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