Analysis of terpenes from ginkgo biloba L. by high-performance liquid chromatography

Radiolabeling of ginkgolide B with 18F

“…The purification of 7-18 FGB was performed on a C18 semi-preparative HPLC column (Figure 3). 7-18 FGB, which eluted at approximately 17 min (Peak c), was well separated from unreacted 18 F and radioactive by-products, which eluted near the void volume (Peak a and b). The small peak at the retention time of 2.3 min (Peak b) might be derived from 7-18 FGB with its lactone ring(s) open.…”

“…Raising the acetonitrile composition of the mobile phase from 20 to 30% resulted in lowering the radiochemical purity of the final product to approximately 70%. HPLC analyses suggested that this was caused by unreacted 18 F tailing into the 7-18 FGB elution, which peaked at UV characteristics of ginkgolides such as the lack of absorption maxima above 200 nm and low molar absorptivities make quantitative HPLC analyses of these compounds with UV detection difficult: at l max 219 (ethanol), their molar absorptivities range from 200 to 500 l/mol cm. 16 Usable solvents are also limited.…”

“…Based on this notion, we radiolabeled ginkgolide B (GB) with 18 F. We selected ginkgolide B (Figure 1) over other ginkgolides because of availability of a relatively large volume of literature focusing on CNS effects of ginkgolide B.…”

Chemistry and Biology of Terpene Trilactones fromGinkgo Biloba

“…The first reported separation and quantification of TTLs, however, involved conventional HPLC/UV detection. [88] RI detection was successfully applied later. [89,90] Although UV detection is often preferred [91] as it demonstrates better sensitivity than RI detection, the selectivity is superior when using RI.…”

Development and Validation of a Gas Chromatographic Method for Identification and Quantification of Terpene Trilactones in Ginkgo biloba L. Extract and Pharmaceutical Preparations~!2009-10-28~!2010-01-15~!2010-06-09~!

“…However, they have poor chromophores and can only be monitored at low and non selective wavelengths (190-220 nm). This makes UV detection difficult, interfered with trace impurities especially when analyzing crude Ginkgo extracts and extensive cleanup procedures that are usually required as prior to analyze leaf extracts and dosage forms [23][24][25][26]. In spite of this detection difficulty, an Egyptian group validated and published a RP-HPLC method using UV detection at 220 nm [27].…”