Development and Validation of a Gas Chromatographic Method for Identification and Quantification of Terpene Trilactones in Ginkgo biloba L. Extract and Pharmaceutical Preparations~!2009-10-28~!2010-01-15~!2010-06-09~!

Sayadi, Lotfi; Missaoui, Ines; Jamoussi, Bassem; Abderraba, Abdelmanef

doi:10.2174/1875038901003010018

Cited by 3 publications

References 31 publications

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Plant Sesquiterpenes and other Terpenoids

Kouloura

Tchoumtchoua

Halabalaki

et al. 2014

Encyclopedia of Analytical Chemistry

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Terpenoids constitute one of the most numerous and structurally varied chemical group of natural products (>40,000) broadly abundant in the plant kingdom. Among them, sesquiterpenoids and other related low mass terpenoids encompass a largely studied and widely distributed subgroup. Apart from their essential physiological role in plant organisms, these secondary metabolites demonstrate important physicochemical and biological properties, enabling their exploitation as medicinal, cosmetic, or agrochemical agents. Mainly owing to their unique structural features, they comprise a highly promising area among natural products and, especially, in the discovery of novel pharmacological active compounds. During this effort, several analytical techniques, approaches, and methodologies have been applied, and numerous new have been lately introduced. In this chapter, the main techniques that are incorporated in the analysis of sesquiterpenoids and small terpenoids are presented with emphasis in recent advances and approaches. Specifically, new extraction and separation techniques, methodologies for the analysis and characterization, and detection and identification of sesquiterpenoids and related compounds are discussed using examples from the plant kingdom.

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Plant Sesquiterpenes and other Terpenoids

Kouloura

Tchoumtchoua

Halabalaki

et al. 2014

Encyclopedia of Analytical Chemistry

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Development and validation of an ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry method for the simultaneous determination of selected flavonoids in Ginkgo biloba

Pandey

Chandra

Arya

et al. 2014

J of Separation Science

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A rapid and sensitive ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry method was developed and validated for the simultaneous determination of 13 flavonoids in leaf, stem, and fruit extracts of male and female trees of Ginkgo biloba to investigate gender- and age-related variations of flavonoids content. Chromatographic separation was accomplished on an Acquity UPLC BEH C18 column (50 mm × 2.1 mm id, 1.7 μm) in 5 min. Quantitation was performed using negative electrospray ionization mass spectrometry in multiple reaction monitoring mode. The calibration curves of all analytes showed a good linear relationship (r(2) ≥ 0.9977) over the concentration range of 1-1000 ng/mL. The precision evaluated by an intra- and interday study showed RSD ≤ 1.98% and good accuracy with overall recovery in the range from 97.90 to 101.09% (RSD ≤ 1.67%) for all analytes. The method sensitivity expressed as the limit of quantitation was typically 0.25-3.57 ng/mL. The results showed that the total content of 13 flavonoids was higher in the leaf extract of an old male Ginkgo tree compared to young female Ginkgo trees.

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Analytical Investigation of Active Compound Contents of Panax ginseng C.A. Meyer and Ginkgo biloba L. Supplements Fortified with Apitherapy Products

Akyıldız

Raday²,

Erdem³

et al. 2021

International Journal of Secondary Metabolite

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Extracts of therapeutic plants of nature, such as Ginkgo biloba L. and Panax ginseng C.A. Meyer (P.ginseng) are highly requested. Recently admixtures of these extracts with apitherapy products are also available. In this study, as research materials, P.ginseng, and Ginkgo biloba L. supplements in the form of tablets, paste, and liquid extracts prepared as a mixture with bee products were compared in terms of their ginsenosides, phenolics, terpene lactones, and antioxidant capacities. Within this study, it was aimed to clarify the active ingredients P.ginseng extracts, Ginkgo biloba L. extracts, and their mixtures with bee products by developing advanced and novel analytical methods. Additionally, it was purposed to unveil the contribution of bee products to phytotherapy supplements of P.ginseng and Ginkgo biloba L. on the basis of secondary metabolites. Ultrasound-assisted hydroalcoholic extraction was applied at sample preparation and supernatants were analyzed at UPLC-MS/MS and UV-Visible spectrophotometer. The antioxidant activities were determined using the 2,2diphenyl-1-picrylhydrazyl (DPPH) method. As a result, total ginsenoside contents of the products per serving were ranged between 9.7 mg to 150.2 mg. Terpene lactones of the ginkgo products were ranged between 0.1 mg to 9.5 mg per serving. The antioxidant activities of all products were determined between 13% and 92%. Quercetin, rutin hydrate, CAPE, kaempferol, galangin, chrysin, gallic acid, pinocembrin, and isorhamnetin were found as the predominant phenolics. Due to their higher antioxidant capacities, superior phenolic concentrations, and diversities, it was enlightened that apitherapy products can augment the phytotherapy efficacies of P.ginseng and Ginkgo biloba L. supplements.

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Development and Validation of a Gas Chromatographic Method for Identification and Quantification of Terpene Trilactones in Ginkgo biloba L. Extract and Pharmaceutical Preparations~!2009-10-28~!2010-01-15~!2010-06-09~!

Cited by 3 publications

References 31 publications

Plant Sesquiterpenes and other Terpenoids

Plant Sesquiterpenes and other Terpenoids

Development and validation of an ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry method for the simultaneous determination of selected flavonoids in Ginkgo biloba

Analytical Investigation of Active Compound Contents of Panax ginseng C.A. Meyer and Ginkgo biloba L. Supplements Fortified with Apitherapy Products

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